Issue 9, 2016

Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies

Abstract

The transparency of the human eye lens depends on the solubility and stability of the structural proteins of the eye lens, the crystallins. Although the mechanism of cataract formation is still unclear, it is believed to involve protein misfolding and/or aggregation of proteins due to the influence of several external factors such as ultraviolet (UV) radiation, low pH, temperature and exposure to chemical agents. In this article, we report the study of UV induced photo-damage (under oxidative stress) of recombinant human γB-crystallin in vitro in the presence of the major green tea polyphenol, (−)-epigallocatechin gallate (EGCG). We have shown that EGCG has the ability to protect human γB-crystallin from oxidative stress-induced photo-damage.

Graphical abstract: Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies

Article information

Article type
Paper
Submitted
06 四月 2016
Accepted
28 六月 2016
First published
28 六月 2016

Mol. BioSyst., 2016,12, 2901-2909

Protection of human γB-crystallin from UV-induced damage by epigallocatechin gallate: spectroscopic and docking studies

S. Chaudhury, S. Bag, M. Bose, A. K. Das, A. K. Ghosh and S. Dasgupta, Mol. BioSyst., 2016, 12, 2901 DOI: 10.1039/C6MB00256K

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