Issue 6, 2016

TMEM45B promotes proliferation, invasion and migration and inhibits apoptosis in pancreatic cancer cells

Abstract

In the present study, we focused on the expression and biological functions of TMEM45B in pancreatic cancer tissues and cell lines. Real-time PCR and Western blotting were used to examine the expression levels of TMEM45B in pancreatic cancer tissues and cell lines. The functions of TMEM45B were evaluated using CCK-8, flow cytometry and transwell analysis. Our results showed that TMEM45B exhibited high expression in pancreatic cancer tissues and cell lines compared with the normal pancreatic tissues and cells. Using gene set enrichment analysis (GSEA), we found that TMEM45B may regulate multiple genes involved in the cell cycle and metastasis pathways. Downregulation of TMEM45B by RNA interference significantly reduced proliferation, invasion and migration of SW1990 and PANC-1 cells, accompanied by the induction of cell cycle arrest and apoptosis, whereas overexpression of TMEM45B promoted proliferation, invasion and migration of CFPAC-1 cells as well as apoptosis inhibition. Taken together, our study provides evidence that TMEM45B is an oncogene involved in the tumorigenesis of pancreatic cancer and may represent a new molecular target for pancreatic cancer treatment.

Graphical abstract: TMEM45B promotes proliferation, invasion and migration and inhibits apoptosis in pancreatic cancer cells

Article information

Article type
Paper
Submitted
18 三月 2016
Accepted
11 四月 2016
First published
25 四月 2016

Mol. BioSyst., 2016,12, 1860-1870

TMEM45B promotes proliferation, invasion and migration and inhibits apoptosis in pancreatic cancer cells

L. Zhao, B. Shen, X. Deng, H. Chen, Z. Zhu and C. Peng, Mol. BioSyst., 2016, 12, 1860 DOI: 10.1039/C6MB00203J

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