Issue 11, 2015

Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

Abstract

Direct visualization of bioorthogonal alkyne or azide handles using fluorogenic azide–alkyne cycloaddition conducted on the surface of a blot membrane. The method eliminates the need for separation steps to remove excess small molecule reagents before attachment of antigen molecules or other visualization handles, and is especially useful for the analysis of peptides and small proteins. A variety of potential fluorogenic reagents are assessed, and sensitivity (<0.1 picomole) similar to current commercially available fluorescence imaging methods is possible.

Graphical abstract: Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

Supplementary files

Article information

Article type
Communication
Submitted
29 七月 2015
Accepted
20 八月 2015
First published
24 八月 2015

Mol. BioSyst., 2015,11, 2846-2849

Author version available

Convenient analysis of protein modification by chemical blotting with fluorogenic “click” reagents

J. Ohata, F. Vohidov and Z. T. Ball, Mol. BioSyst., 2015, 11, 2846 DOI: 10.1039/C5MB00510H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements