Affinity-based thermoresponsive fluorescence switching of proteins conjugated with a polymer-binding peptide

Toshiki Sawada; Seigo Suzuki; Takeshi Serizawa

doi:10.1039/D0SM01107J

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Affinity-based thermoresponsive fluorescence switching of proteins conjugated with a polymer-binding peptide†

Toshiki Sawada,

^ab Seigo Suzuki^a and Takeshi Serizawa

*^a

Author affiliations

* Corresponding authors

^a Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550, Japan
E-mail: serizawa@mac.titech.ac.jp

^b Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi-shi, Saitama 332-0012, Japan

Abstract

The affinity-based thermoresponsive fluorescence switching of proteins conjugated with a polymer-binding peptide is demonstrated. The specific affinity of the peptide and thermoresponsive structural transitions of the polymer are essential for reliable fluorescence switching behavior.

This article is part of the themed collection: Peptide Soft Materials

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Supplementary files

Supplementary information PDF (1598K)

Article information

DOI: https://doi.org/10.1039/D0SM01107J
Article type: Communication
Submitted: 16 六月 2020
Accepted: 28 七月 2020
First published: 30 七月 2020

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Soft Matter, 2020,16, 10096-10100

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Affinity-based thermoresponsive fluorescence switching of proteins conjugated with a polymer-binding peptide

T. Sawada, S. Suzuki and T. Serizawa, Soft Matter, 2020, 16, 10096 DOI: 10.1039/D0SM01107J

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Soft Matter

Affinity-based thermoresponsive fluorescence switching of proteins conjugated with a polymer-binding peptide†

Abstract

Supplementary files

Article information

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Affinity-based thermoresponsive fluorescence switching of proteins conjugated with a polymer-binding peptide

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