Issue 33, 2019

An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells

Abstract

An ultra-sensitive and ratiometric fluorescent probe for hypochlorous acid (HOCl) detection based on the mechanism of aggregation induced emission (AIE) and through-bond energy transfer (TBET) has been reported herein. By exploiting the advantages of AIE and TBET, which eliminates emission leakage from dark donors, the probe exhibits ultra-high sensitivity towards HOCl by an enhancement of over 7000-fold in the fluorescence ratio (I589 nm/I477 nm), which is one of the highest recorded so far. The reaction mechanism has been discussed in detail, and the effects of interferents and the reaction kinetics have also been investigated. Lastly, the successful result of exogenous/endogenous HOCl imaging detection in different cell lines indicates the potential use of the probe in living systems.

Graphical abstract: An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells

Supplementary files

Article information

Article type
Paper
Submitted
25 六月 2019
Accepted
20 七月 2019
First published
20 七月 2019

J. Mater. Chem. B, 2019,7, 5125-5131

An ultra-sensitive ratiometric fluorescent probe for hypochlorous acid detection by the synergistic effect of AIE and TBET and its application of detecting exogenous/endogenous HOCl in living cells

Q. Duan, G. Zheng, Z. Li, K. Cheng, J. Zhang, L. Yang, Y. Jiang, H. Zhang, J. He and H. Sun, J. Mater. Chem. B, 2019, 7, 5125 DOI: 10.1039/C9TB01279F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements