Issue 7, 2015

Raman microimaging of murine lungs: insight into the vitamin A content

Abstract

The composition of the lung tissue of mice was investigated using Raman confocal microscopy at 532 nm excitation wavelength and was supported with various staining techniques as well as DFT calculations. This combination of experimental and theoretical techniques allows for the study of the distribution of lung lipofibroblasts (LIFs), rich in vitamin A, as well as the chemical structure of vitamin A. The comparison of the Raman spectra derived from LIFs with the experimental and theoretical spectra of standard retinoids showed the ability of LIFs to store all-trans retinol, which is partially oxidized to all-trans retinal and retinoic acid. Moreover, we were able to visualize the distribution of other lung tissue components including the surfactant and selected enzymes (lipoxygenase/glucose oxidase).

Graphical abstract: Raman microimaging of murine lungs: insight into the vitamin A content

Supplementary files

Article information

Article type
Paper
Submitted
17 十月 2014
Accepted
11 十二月 2014
First published
11 十二月 2014

Analyst, 2015,140, 2171-2177

Author version available

Raman microimaging of murine lungs: insight into the vitamin A content

K. M. Marzec, K. Kochan, A. Fedorowicz, A. Jasztal, K. Chruszcz-Lipska, J. Cz. Dobrowolski, S. Chlopicki and M. Baranska, Analyst, 2015, 140, 2171 DOI: 10.1039/C4AN01881H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements