Mercury modulates selenium activity via altering its accumulation and speciation in garlic (Allium sativum)

Abstract

Combined pollution of selenium (Se) and mercury (Hg) has been known in Wanshan district (Guizhou Province, China). A better understanding of how Se and Hg interact in plants and the phytotoxicity thereof will provide clues about how to avoid or mitigate adverse effects of Se/Hg on local agriculture. In this study, the biological activity of Se has been investigated in garlic with or without Hg exposure. Se alone can promote garlic growth at low levels (<0.1 mg L⁻¹), whereas it inhibits garlic growth at high levels (>1 mg L⁻¹). The promotive effect of Se in garlic can be enhanced by low Hg exposure (<0.1 mg L⁻¹). When both Se and Hg are at high levels, there is a general antagonistic effect between these two elements in terms of phytotoxicity. Inductively coupled plasma mass spectrometry (ICP-MS) data suggest that Se is mainly concentrated in garlic roots, compared to the leaves and the bulbs. Se uptake by garlic in low Se medium (<0.1 mg L⁻¹) can be significantly enhanced as Hg exposure levels increase (P < 0.05), while it can be inhibited by Hg when Se exposure levels exceed 1 mg L⁻¹. The synchrotron radiation X-ray fluorescence (SRXRF) mapping further shows that Se is mainly concentrated in the stele of the roots, bulbs and the veins of the leaves, and Se accumulation in garlic can be reduced by Hg. The X-ray absorption near edge structure (XANES) study indicates that Se is mainly formed in C–Se–C form in garlic. Hg can decrease the content of inorganic Se mainly in SeO₃²⁻ form in garlic while increasing the content of organic Se mainly in C–Se–C form (MeSeCys and its derivatives). Hg-mediated changes in Se species along with reduced Se accumulation in garlic may account for the protective effect of Hg against Se phytotoxicity.