Immunoaffinity assisted LC-ICP-MS—a versatile tool in biomedical research

Abstract

A novel concept of combining immunochemistry, i.e., the specific recognition of antigens by antibodies, with elemental speciation analysis has potential use for targeted protein and peptide quantification in complex biological matrices. Accuracy, sensitivity and high selectivity is a major demand in this challenging task, both will be achieved when separating elemental labelled antigen-antibody complexes in solution via liquid chromatography in combination with inductively coupled plasma-mass spectroscopy (ICP-MS) detection. The on-line approach is rapid and automatable.