Themed collection Materials Chemistry of Fluorescence Bioimaging

Steric protection of a long-wavelength dye, by covalent steric appendage or non-covalent encapsulation, produces enhanced photostability, increased fluorescence brightness, decreased self-aggregation, and superior pharmacokinetic profile.

This review first outlines the advantages of fluorescence imaging in the NIR-II region, followed by a comprehensive overview of recent advancements in NIR-II fluorophores and their versatile applications in biomedical imaging.

AI-driven precision subcellular navigation with fluorescent probes.

This review summarizes various probes for imaging LDs in the fatty liver process using different excitation imaging modes and emission wavelengths, as well as analyzing the achievements, challenges and future development trends in this field.

This review summarizes the recent advancements in biomedical applications of dual-/multi-organelle-targeted aggregation-induced emission (AIE) probes, focusing on their in situ induction of oxidative stress.

A guanidiniocarbonyl-pyrrole functionalized cucurbit[7]uril derivative as a cytomembrane disruptor has been successfully constructed for synergistic antibacterial therapy.

Light-activated fluorescence represents a potent tool for investigating subcellular structures and dynamics, offering enhanced control over the temporal and spatial aspects of the fluorescence signal.

γ-Glutamyl transpeptidase (GGT) regulates glutathione (GSH), essential for cell functions and linked to cancer.

The new 5-element porphyrin block copolymer sensor array allows simultaneous cell imaging and tumor type identification, offering promise for clinical applications.

We present the synthesis of two TFMDSA nanoparticles, demonstrating outstanding luminescence efficiency and stability. These nanoparticles were subsequently employed in cellular imaging applications, yielding satisfactory imaging outcomes.

We propose a DNAzyme walker constructed from aptamer-modified DNA nanowires, which can efficiently penetrate target cells without the assistance of non-DNA substances. This strategy enables sensitive and effective imaging of miRNA in tumor cells.

Based on the cyano positional isomerism strategy, a cationic AIEgen was developed, which can specifically stain mitochondria, and ablate cancer cells through potent type I photodynamic therapy both in vitro and in multicellular tumor spheroid models.

A mitochondria targeting, de novo designed, AIE-based Cou-AIE-TPP⁺ probe for monitoring Aβ aggregation kinetics, imaging Aβ aggregates, and detecting mitochondrial morphology change and dysfunction during Aβ-induced neurotoxicity.

An NIR emissive probe plays a pivotal role for in vitro and in vivo dead cell sorting.

A novel mitochondrial targeting mitochondrial membrane potential-independent near-infrared fluorescent probe, ACR-DMA, was developed which can be firmly immobilized in mitochondria for tracking of mitochondrial viscosity changes in vitro and in vivo.

A H₂O₂-activatable Bodipy with simultaneous type I and type II photosensitization was designed for tumor imaging and therapy.

A dual-functional photosensitizer was developed, exhibiting potent phototoxicity and sensitivity to polarity changes.

The isomerization effect on coumarin-based AIEgens was systematically analyzed and further applied to H₂S detection.

Peptide-conjugated cis/trans isomers were designed. cis-RTP interacts more stably with cell membranes than trans-RTP, and it has shown more excellent properties in inhibiting cell migration and killing cells.

Rare examples of ICT-based fluorescent probes incorporating phosgene specific recognition units at the 2-position of the BODIPY core.

Indocyanine green nanoparticles are formed through co-assembly with an amino acid derivative, achieving both its fluorescence and photothermal properties on nanoplatforms for fluorescence imaging-guided photothermal therapy against tumors.

BSe-B is a novel photosensitizer for photodynamic therapy using two-photon excitation, selectively targets cancer cell while sparing healthy tissue. It generates type-I reactive oxygen species and shows promise in diagnosis and treatment of cancer.

We present novel bis-acridine orange (BAO) dyes that outperform EvaGreen in qPCR over a wider concentration range and show great potential as candidates for bioimaging. The enhanced qPCR performance of the BAO dyes is supported by MD simulations.

Cyclodextrins, saccharides, and ethanol were examined to assess the effects of hydroxy groups on the ESPT reaction. Differences in the fluorescent behaviors indicate varying interactions between the hydroxy compounds and complexes.

Potassium cations (K⁺) play key roles in organisms. Fluorescent ratiometric microsensors for K⁺ were synthesized using silica microparticles. Their spectral and physicochemical properties were fully investigated for K⁺ detection and tracking.

We report the development of a hydroxyl radical responsive nanoprobe based on clay nanoparticles for ratiometric fluorescence determination and imaging of macrophage polarization induced by biomolecules and ultrasound stimulations.

Fluorescent probes target serotonin transporter and discern H₂O₂ in serotonergic neurons in living brains of mice.

A NO-induced urea-bond cleavage reaction is developed and utilized to generally construct fluorescent probes for imaging NO in living systems.

Current clinical indicators for assessing liver/kidney injury are functional rather than injury indicators, which may cause some delays in the diagnosis of drug-induced liver injury (DILI) and kidney injury (DIKI).

Organelle-resolved imaging of formaldehyde reveals the different responses of various organelles to the same cell stressor.

Proximity labeling expansion microscopy (PL-ExM) visualizes superresolution structures of interactome on widely accessible light microscopes, enabling the assessment of the precision and efficiency of proximity labeling techniques.

We successfully developed naphthalene-based fluorescent probe 1 for detection of ultra-low concentrations of Hg²⁺via the formation of excimer, which resulted in emitted fluorescence.

Transient absorption spectroscopy has revealed that AIEgens exhibit a more rapid and facile excited-state molecular motion in the solution state compared to the aggregate state, implying the predominance of nonradiative decay in the solution state.

New fluorescent carbon nanoparticles, obtained using artichoke extract, have been used for the ultrasensitive (sub-nanomolar) and selective detection of dopamine in artificial saliva samples.

A small molecule organic fluorophore has been developed to monitor the micropolarity inside endoplasmic reticulum in homeostatic and non-homeostatic conditions and visualize ER to Golgi transport.

A turn-on estrogen receptor-positive (ER+) NIR fluorescent probe NB-TAM was prepared and successfully applied to clearly delineate the ER+ breast tumor incisal margin and identify very tiny metastatic tumor foci at high resolution.

A novel fluorescent probe, AGO, systematically detects advanced glycation end products involved in aging.

Regulation effects of three aniline-based squaraines were investigated. The first expression of distinct tissue labelling and high-resolution 3D reconstruction in the onion and plant cells induced by the subtle structure changes was further evaluated.

The ability to detect and visualize cellular events and associated biological analytes is essential for the understanding of their physiological and pathological functions.