Issue 7, 2024

Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling

Abstract

Microbial rhodopsin (MRs) ion channels and pumps have become invaluable optogenetic tools for neuroscience as well as biomedical applications. Recently, MR-optogenetics expanded towards subcellular organelles opening principally new opportunities in optogenetic control of intracellular metabolism and signaling via precise manipulations of organelle ion gradients using light. This new optogenetic field expands the opportunities for basic and medical studies of cancer, cardiovascular, and metabolic disorders, providing more detailed and accurate control of cell physiology. This review summarizes recent advances in studies of the cellular metabolic processes and signaling mediated by optogenetic tools targeting mitochondria, endoplasmic reticulum (ER), lysosomes, and synaptic vesicles. Finally, we discuss perspectives of such an optogenetic approach in both fundamental and applied research.

Graphical abstract: Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling

Supplementary files

Article information

Article type
Review Article
Submitted
27 8月 2023
First published
23 2月 2024

Chem. Soc. Rev., 2024,53, 3327-3349

Intracellular microbial rhodopsin-based optogenetics to control metabolism and cell signaling

A. D. Vlasova, S. M. Bukhalovich, D. F. Bagaeva, A. P. Polyakova, N. S. Ilyinsky, S. V. Nesterov, F. M. Tsybrov, A. O. Bogorodskiy, E. V. Zinovev, A. E. Mikhailov, A. V. Vlasov, A. I. Kuklin, V. I. Borshchevskiy, E. Bamberg, V. N. Uversky and V. I. Gordeliy, Chem. Soc. Rev., 2024, 53, 3327 DOI: 10.1039/D3CS00699A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements