Issue 22, 2019

A single nanowire sensor for intracellular glucose detection

Abstract

Glucose metabolism plays an important role in cell energy supply, and quantitative detection of the intracellular glucose level is particularly important for understanding many physiological processes. Glucose electrochemical sensors are widely used for blood and extracellular glucose detection. However, intracellular glucose detection cannot be achieved by these sensors owing to their large size and consequent low spatial resolution. Herein, we developed a single nanowire glucose sensor for electrochemical detection of intracellular glucose by depositing Pt nanoparticles (Pt NPs) on a SiC@C nanowire and further immobilizing glucose oxidase (GOD) thereon. Glucose was converted by GOD to an electroactive product H2O2 which was further electro-catalyzed by Pt NPs. The glucose nanowire sensor is endowed with a high sensitivity, high spatial-temporal resolution and enzyme specificity due to its nanoscale size and enzymatic reaction. This allows the real-time monitoring of the intracellular glucose level, and the increase of the intracellular glucose level induced by a novel potential hypoglycemic agent, reinforcing its potential application in lowering the blood glucose level. This work provides a versatile method for the construction of enzyme-modified nanosensors to electrochemically detect intracellular non-electroactive molecules, which is of great benefit for physiological and pathological studies.

Graphical abstract: A single nanowire sensor for intracellular glucose detection

  • This article is part of the themed collection: Nanocarbons

Supplementary files

Article information

Article type
Paper
Submitted
06 3月 2019
Accepted
30 4月 2019
First published
07 5月 2019

Nanoscale, 2019,11, 10702-10708

A single nanowire sensor for intracellular glucose detection

Q. Liao, H. Jiang, X. Zhang, Q. Qiu, Y. Tang, X. Yang, Y. Liu and W. Huang, Nanoscale, 2019, 11, 10702 DOI: 10.1039/C9NR01997A

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