Issue 2, 2019

Interfacial engineering of carbon dots with benzenediboronic acid for fluorescent biosensing

Abstract

Glucose assay is highly important in clinical diagnostics of diabetes. Herein, we engineered the surface of carbon dots by complexation with functional ligand and constructed fluorescent biosensors for the detection of hydrogen peroxide and glucose. In this study, benzenediboronic acid is conjugated to the surface of citric acid-derived carbon dots through formation of boronate complexes with the nanoparticles. The oxidation of benzenediboronic acid with hydrogen peroxide effectively quenches fluorescence of carbon dots through electron transfer process. The sensing performance of the system according to different engineered surfaces of carbon dots was studied by using carbon dots derived from various precursors and different benzenediboronic acid analogues. As a simple mix-and-detect strategy, this system is facilely applied for glucose sensing as hydrogen peroxide is the product catalyzed by glucose oxidase. The benzenediboronic acid-conjugated carbon dots derived from citric acid act as excellent optical probes for sensitive analysis of glucose with detection limit of 0.4 μM. This sensing system shows great selectivity toward interferent species such as analogues of glucose, and can be used to determine glucose in human serum. Engineering the surface of carbon dots by complexation with ligand of interest provides a feasible way to facilitate the development of biological applications.

Graphical abstract: Interfacial engineering of carbon dots with benzenediboronic acid for fluorescent biosensing

Supplementary files

Article information

Article type
Paper
Submitted
23 8月 2018
Accepted
01 11月 2018
First published
06 11月 2018
This article is Open Access
Creative Commons BY-NC license

Nanoscale Adv., 2019,1, 765-771

Interfacial engineering of carbon dots with benzenediboronic acid for fluorescent biosensing

M. Pan, Z. Xu, Q. Jiang, J. Feng, J. Sun, F. Wang and X. Liu, Nanoscale Adv., 2019, 1, 765 DOI: 10.1039/C8NA00166A

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