Issue 14, 2019

A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

Abstract

Developing efficient methods for the real-time detection of Zn2+ levels in biological systems is highly relevant to improving our understanding of the role of Zn2+ in the progression of Parkinson's disease (PD). In this work, a novel Schiff base based Zn2+ fluorescent probe (ZP) was designed, synthesized and systematically investigated. A significant turn-on effect on ZP upon the addition of Zn2+ was observed, accompanied by a blue-shift of the fluorescence spectra. ZP is sensitive to Zn2+ and has excellent selectivity against various biologically relevant cations, anions and amino acids. The sensing mechanism of ZP was studied by 1H NMR, MS, single crystal X-ray diffraction and theoretical calculations. The results showed that the response of ZP to Zn2+ was based on the chelation–hydrolysis-enhancement process. Upon bonding, Zn2+ hydrolyzes the Schiff base to an aldehyde precursor, the resulting aldehyde further coordinates to Zn2+ to form a more stable heterobimetallic complex leading to the emission enhancement and blue-shift. ZP was applied to imaging exogenous/endogenous Zn2+ in live HeLa cells. Furthermore, we successfully measured the Zn2+ levels using in vitro PD models, which provided a visualization method to better understand the relationship between Zn2+ levels and PD development.

Graphical abstract: A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

Supplementary files

Article information

Article type
Paper
Submitted
24 12月 2018
Accepted
23 1月 2019
First published
24 1月 2019

J. Mater. Chem. B, 2019,7, 2252-2260

A fluorogenic probe based on chelation–hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models

G. Zhang, Y. Zhao, B. Peng, Z. Li, C. Xu, Y. Liu, C. Zhang, N. H. Voelcker, L. Li and W. Huang, J. Mater. Chem. B, 2019, 7, 2252 DOI: 10.1039/C8TB03343A

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