Issue 13, 2016

Novel reduced graphene oxide–glycol chitosan nanohybrid for the assembly of an amperometric enzyme biosensor for phenols

Abstract

A novel water-soluble graphene derivative was prepared from graphene oxide via a two-step modification approach. Graphene oxide was first functionalised with reactive epoxy groups by covalent modification with (3-glycidyloxypropyl)trimethoxysilane and further cross-linked with glycol chitosan. This graphene derivative was characterized using different microscopy and physicochemical methods and employed as a coating material for a glassy carbon electrode. The nanostructured surface was used as a support for the covalent immobilization of the enzyme laccase through cross-linking with glutaraldehyde. The enzyme electrode was tested for the amperometric detection of different phenolic compounds, which displayed excellent analytical behaviour toward catechol with a linear range of response from 200 nM to 15 μM, sensitivity of 93 mA M−1 cm−2, and low detection limit of 76 nM. The enzyme biosensor showed high stability when stored at 4 °C under dry conditions and was successfully employed to quantify the total phenolic compounds in commercial herbal tea samples.

Graphical abstract: Novel reduced graphene oxide–glycol chitosan nanohybrid for the assembly of an amperometric enzyme biosensor for phenols

Supplementary files

Article information

Article type
Paper
Submitted
24 12月 2015
Accepted
29 4月 2016
First published
29 4月 2016

Analyst, 2016,141, 4162-4169

Novel reduced graphene oxide–glycol chitosan nanohybrid for the assembly of an amperometric enzyme biosensor for phenols

A. Boujakhrout, S. Jimenez-Falcao, P. Martínez-Ruiz, A. Sánchez, P. Díez, J. M. Pingarrón and R. Villalonga, Analyst, 2016, 141, 4162 DOI: 10.1039/C5AN02640G

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