Issue 6, 2023

Crystallization-based downstream processing of ω-transaminase- and amine dehydrogenase-catalyzed reactions

Abstract

Biocatalytic synthesis is a powerful and frequently chosen method for the production of chiral amines. Unfortunately, these biocatalytic reactions often result in complex mixtures, bearing many components aside from the main product amine such as residual co-substrates, co-products, cofactors and buffer salts. This issue typically requires an additional effort during downstream processing towards the isolation of the desired chiral amine. For instance, transaminase- and amine dehydrogenase-catalyzed reactions, which often use high surpluses of amine or ammonia co-substrates, face complications in removing the residual amine donor or unreacted substrate and salts from the isolated amine products, thus complicating and increasing the costs of the process of product isolation and purification. This study explores the selective removal of chiral amines from model amine transaminase and amine dehydrogenase-catalyzed reactions via a salt-based specific crystallization step. The product amine is precipitated directly in one step from the reaction mixture as a product ammonium salt, which can easily be filtered from the reaction mixture, while the other reactants remain unchanged in solution for potential re-use.

Graphical abstract: Crystallization-based downstream processing of ω-transaminase- and amine dehydrogenase-catalyzed reactions

Supplementary files

Article information

Article type
Paper
Submitted
14 11月 2022
Accepted
21 3月 2023
First published
24 3月 2023
This article is Open Access
Creative Commons BY-NC license

React. Chem. Eng., 2023,8, 1427-1439

Crystallization-based downstream processing of ω-transaminase- and amine dehydrogenase-catalyzed reactions

F. Belov, A. Mildner, T. Knaus, F. G. Mutti and J. von Langermann, React. Chem. Eng., 2023, 8, 1427 DOI: 10.1039/D2RE00496H

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