Issue 5, 2023

Induction of toxicity in human colon cells and organoids by size- and composition-dependent road dust

Abstract

Environmental pollution, including the annual resurgence of particulate matter derived from road dust, is a serious issue worldwide. Typically, the size of road dust is less than 10 μm; thus, road dust can penetrate into human organs, including the brain, through inhalation and intake by mouth. Therefore, the toxicity of road dust has been intensively studied in vitro and in vivo. However, in vitro systems, including 2D cell cultures, cannot mimic complex human organs, and there are several discrepancies between in vivo and human systems. Here, we used human colon cells and organoids to evaluate the cytotoxicity of particulate matter derived from road dust. The toxicity of road dust collected in industrialized and high traffic areas and NIST urban particulate matter reference samples were evaluated in 2D and 3D human colon cells as well as colon organoids and their characteristics were carefully examined. Data suggest that the size and elemental compositions of road dust can correlate with colon organoid toxicity, and thus, a more careful assessment of the size and elemental compositions of road dust should be conducted to predict its effect on human health.

Graphical abstract: Induction of toxicity in human colon cells and organoids by size- and composition-dependent road dust

Supplementary files

Article information

Article type
Paper
Submitted
25 11月 2022
Accepted
03 1月 2023
First published
18 1月 2023
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2023,13, 2833-2840

Induction of toxicity in human colon cells and organoids by size- and composition-dependent road dust

S. B. Park, E. Kim, K. Y. Kim and B. Koh, RSC Adv., 2023, 13, 2833 DOI: 10.1039/D2RA07500H

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