Volume 232, 2021

The impact of antibacterial peptides on bacterial lipid membranes depends on stage of growth

Abstract

The impact of maculatin 1.1 (Mac1) on the mechanical properties of supported lipid membranes derived from exponential growth phase (EGP) and stationary growth phase (SGP) E. coli lipid extracts was analysed by surface plasmon resonance and atomic force microscopy. Each membrane was analysed by quantitative nanomechanical mapping to derive measurements of the modulus, adhesion and deformation in addition to bilayer height. Image analysis revealed the presence of two domains in the EGP membrane differing in height by 0.4 nm. Three distinct domains were observed in the SGP membrane corresponding to 4.2, 4.7 and 5.4 nm in height. Using surface plasmon resonance, Mac1 was observed to bind strongly to both membranes and then disrupt the membranes as evidenced by a sharp drop in baseline. Atomic force microscopy (AFM) topographic analysis revealed the formation of domains of different height and confirmed that membrane destruction was much faster for the SGP derived bilayer. Moreover, Mac1 selectively disrupted the domain with the lowest thickness, which may correspond to a liquid ordered domain. Overall, the results provide insight into the role of lipid domains in the response of bacteria to antimicrobial peptides.

Graphical abstract: The impact of antibacterial peptides on bacterial lipid membranes depends on stage of growth

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
04 5月 2020
Accepted
24 6月 2020
First published
25 6月 2020

Faraday Discuss., 2021,232, 399-418

The impact of antibacterial peptides on bacterial lipid membranes depends on stage of growth

T. Lee, V. Hofferek, M. Sani, F. Separovic, G. E. Reid and M. Aguilar, Faraday Discuss., 2021, 232, 399 DOI: 10.1039/D0FD00052C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements