Effective bioprinting resolution in tissue model fabrication

Amir K. Miri; Iman Mirzaee; Shabir Hassan; Shirin Mesbah Oskui; Daniel Nieto; Ali Khademhosseini; Yu Shrike Zhang

doi:10.1039/C8LC01037D

Effective bioprinting resolution in tissue model fabrication†

Amir K. Miri,^abc Iman Mirzaee,^d Shabir Hassan,‡^ab Shirin Mesbah Oskui,

‡^abi Daniel Nieto,^ab Ali Khademhosseini

*^efgh and Yu Shrike Zhang

*^ab

Author affiliations

* Corresponding authors

^a Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA
E-mail: yszhang@research.bwh.harvard.edu

^b Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

^c Department of Mechanical Engineering, Rowan University, Glassboro, NJ 08028, USA

^d Department of Mechanical Engineering, University of Massachusetts, Lowell, MA 01854, USA

^e Center for Minimally Invasive Therapeutics (C-MIT), University of California-Los Angeles, Los Angeles, CA 90095, USA
E-mail: khademh@ucla.edu

^f Department of Radiology, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, CA 90095, USA

^g Department of Bioengineering, Department of Chemical and Biomolecular Engineering, Henry Samueli School of Engineering and Applied Sciences, University of California-Los Angeles, Los Angeles, CA 90095, USA

^h California NanoSystems Institute (CNSI), University of California-Los Angeles, Los Angeles, CA 90095, USA

ⁱ Bioengineering Program, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA

Abstract

Recent advancements in bioprinting techniques have enabled convenient fabrication of micro-tissues in organ-on-a-chip platforms. In a sense, the success of bioprinted micro-tissues depends on how close their architectures are to the anatomical features of their native counterparts. The bioprinting resolution largely relates to the technical specifications of the bioprinter platforms and the physicochemical properties of the bioinks. In this article, we compare inkjet, extrusion, and light-assisted bioprinting technologies for fabrication of micro-tissues towards construction of biomimetic organ-on-a-chip platforms. Our theoretical analyses reveal that for a given printhead diameter, surface contact angle dominates inkjet bioprinting resolution, while nozzle moving speed and the nonlinearity of viscosity for bioinks regulate extrusion bioprinting resolution. The resolution of light-assisted bioprinting is strongly affected by the photocrosslinking behavior and light characteristics. Our tutorial guideline for optimizing bioprinting resolution would potentially help model the complex microenvironment of biological tissues in organ-on-a-chip platforms.

This article is part of the themed collection: Organ-, body- and disease-on-a-chip systems

Lab on a Chip

Effective bioprinting resolution in tissue model fabrication†

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Effective bioprinting resolution in tissue model fabrication

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