Issue 98, 2016
From the journal:

Chemical Communications

Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes†

A. S. Abdelfattah,a   V. Rancic,b   B. Rawal,b   K. Ballanyib  and  R. E. Campbell*a  

* Corresponding authors

a Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada
E-mail: robert.e.campbell@ualberta.ca

b Department of Physiology, University of Alberta, Edmonton, Alberta, Canada

Abstract

To expand the toolbox of fluorescent protein-based voltage indicators, we explored two distinct protein design strategies. Using these design strategies, we created three new voltage indicators: a red intensiometric voltage indicator (tdFlicR Δ110AR), a green/red ratiometric voltage indicator (tdFlicR-VK-ASAP), and a green to red photoconvertible voltage indicator (FlicGR1).

Graphical abstract: Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C6CC06810C
Article type
Communication
Submitted
19 8月 2016
Accepted
14 11月 2016
First published
14 11月 2016
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2016,52, 14153-14156

Permissions

Request permissions

Ratiometric and photoconvertible fluorescent protein-based voltage indicator prototypes

A. S. Abdelfattah, V. Rancic, B. Rawal, K. Ballanyi and R. E. Campbell, Chem. Commun., 2016, 52, 14153 DOI: 10.1039/C6CC06810C

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements