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A microfluidic-based gut-on-a-chip model containing the gut microbiota of patients with depression reveals physiological characteristics similar to depression
Wenxin Wang, Yiyuan Liu, Zhikai Yao, Dengbo Chen, Yue Tang, Jingwei Cui, Jiangjiang Zhang, Hong Liu and Zikai Hao
Lab Chip, 2024, 24, 2537
DOI: 10.1039/D3LC01052J

Compartmentalized perfusion for temporal control of the chemical microenvironment of iPSC-derived cardiac cells
Kaisa Tornberg, Wolfram Grötsch, Niina Ritari, Saara Haikka, Lassi Sukki, Katriina Aalto-Setälä, Mari Pekkanen-Mattila and Pasi Kallio
Lab Chip, 2025, 25, 2961
DOI: 10.1039/D5LC00072F

Predicting nanocarrier permeation across the human intestine in vitro: model matters
Nathalie Jung, Jonas Schreiner, Florentin Baur, Sarah Vogel-Kindgen and Maike Windbergs
Biomater. Sci., 2024, 12, 5775
DOI: 10.1039/D4BM01092B

Artificial keloid skin models: understanding the pathophysiological mechanisms and application in therapeutic studies
Soo hyun Kwon, Jongmin Lee, Jin Yoo and Youngmee Jung
Biomater. Sci., 2024, 12, 3321
DOI: 10.1039/D4BM00005F

A microfluidic organotypic device for culture of mammalian intestinesex vivo
Alec Richardson, Luke A. Schwerdtfeger, Diana Eaton, Ian Mclean, Charles S. Henry and Stuart A. Tobet
Anal. Methods, 2020, 12, 297
DOI: 10.1039/C9AY02038A

Long-term flow through human intestinal organoids with the gut organoid flow chip (GOFlowChip)
Barkan Sidar, Brittany R. Jenkins, Sha Huang, Jason R. Spence, Seth T. Walk and James N. Wilking
Lab Chip, 2019, 19, 3552
DOI: 10.1039/C9LC00653B

Imaging therapeutic peptide transport across intestinal barriers
Jannik Bruun Larsen, Nayere Taebnia, Alireza Dolatshahi-Pirouz, Anne Zebitz Eriksen, Claudia Hjørringgaard, Kasper Kristensen, Nanna Wichmann Larsen, Niels Bent Larsen, Rodolphe Marie, Ann-Kathrin Mündler, Ladan Parhamifar, Andrew James Urquhart, Arjen Weller, Kim I. Mortensen, Henrik Flyvbjerg and Thomas Lars Andresen
RSC Chem. Biol., 2021, 2, 1115
DOI: 10.1039/D1CB00024A

Organoids/organs-on-a-chip: new frontiers of intestinal pathophysiological models
Lei Wu, Yongjian Ai, Ruoxiao Xie, Jialiang Xiong, Yu Wang and Qionglin Liang
Lab Chip, 2023, 23, 1192
DOI: 10.1039/D2LC00804A

Microfluidic approaches for epithelial cell layer culture and characterisation
Roland Thuenauer, Enrique Rodriguez-Boulan and Winfried Römer
Analyst, 2014, 139, 3206
DOI: 10.1039/C4AN00056K

Advances in modeling periodontal host–microbe interactions: insights from organotypic and organ-on-chip systems
Hardik Makkar and Gopu Sriram
Lab Chip, 2025, 25, 1342
DOI: 10.1039/D4LC00871E

Developingin vitroassays to transform gastrointestinal safety assessment: potential for microphysiological systems
Matthew F. Peters, Allison L. Choy, Carmen Pin, Derek J. Leishman, Annie Moisan, Lorna Ewart, Peggy J. Guzzie-Peck, Radhakrishna Sura, Douglas A. Keller, Clay W Scott and Kyle L. Kolaja
Lab Chip, 2020, 20, 1177
DOI: 10.1039/C9LC01107B

A biologically inspired lung-on-a-chip device for the study of protein-induced lung inflammation
Tushar H. Punde, Wen-Hao Wu, Pei-Chun Lien, Ya-Ling Chang, Ping-Hsueh Kuo, Margaret Dah-Tsyr Chang, Kang-Yun Lee, Chien-Da Huang, Han-Pin Kuo, Yao-Fei Chan, Po-Chen Shih and Cheng-Hsien Liu
Integrative Biology, 2015, 7, 162
DOI: 10.1039/c4ib00239c

From mouth to gut: microfluidic in vitro simulation of human gastro-intestinal digestion and intestinal permeability
Miguel Xavier, Patrícia M. Rodrigues, Mafalda D. Neto, Maria I. Guedes, Victor Calero, Lorenzo Pastrana and Catarina Gonçalves
Analyst, 2023, 148, 3193
DOI: 10.1039/D2AN02088B

Human mesofluidic intestinal model for studying transport of drug carriers and bacteria through a live mucosal barrier
Chia-Ming Wang, Hardeep S. Oberoi, Devalina Law, Yuan Li, Timothy Kassis, Linda G. Griffith, David T. Breault and Rebecca L. Carrier
Lab Chip, 2025, 25, 2990
DOI: 10.1039/D4LC00774C

Abstracting the principles of development using imaging and modeling
Fengzhu Xiong and Sean G. Megason
Integrative Biology, 2015, 7, 633
DOI: 10.1039/c5ib00025d

Microfluidic lumen-based systems for advancing tubular organ modeling
María Virumbrales-Muñoz, José M. Ayuso, Max M. Gong, Mouhita Humayun, Megan K. Livingston, Karina M. Lugo-Cintrón, Patrick McMinn, Yasmín R. Álvarez-García and David J. Beebe
Chem. Soc. Rev., 2020, 49, 6402
DOI: 10.1039/D0CS00705F

Engineering human islet organoids from iPSCs using an organ-on-chip platform
Tingting Tao, Yaqing Wang, Wenwen Chen, Zhongyu Li, Wentao Su, Yaqiong Guo, Pengwei Deng and Jianhua Qin
Lab Chip, 2019, 19, 948
DOI: 10.1039/C8LC01298A

Uptake of silica particulate drug carriers in an intestine-on-a-chip: towards a better in vitro model of nanoparticulate carrier and mucus interactions
Kyall Pocock, Ludivine C. Delon, Aparajita Khatri, Clive Prestidge, Rachel Gibson, Chris Barbe and Benjamin Thierry
Biomater. Sci., 2019, 7, 2410
DOI: 10.1039/C9BM00058E

Enhanced small intestinal organoid-derived epithelial cell adhesion and growth in organ-on-a-chip devices
Federica Quacquarelli, Sergio Davila, Jasin Taelman, Jordi Guiu and Maria Antfolk
RSC Adv., 2025, 15, 3693
DOI: 10.1039/D4RA08290G

Implementation of a dynamic intestinal gut-on-a-chip barrier model for transport studies of lipophilic dioxin congeners
Kornphimol Kulthong, Loes Duivenvoorde, Barbara Z. Mizera, Deborah Rijkers, Guillaume ten Dam, Gerlof Oegema, Tomasz Puzyn, Hans Bouwmeester and Meike van der Zande
RSC Adv., 2018, 8, 32440
DOI: 10.1039/C8RA05430D

Three-dimensional intestinal villi epithelium enhances protection of human intestinal cells from bacterial infection by inducing mucin expression
Si Hyun Kim, Meiying Chi, Banya Yi, So Hyun Kim, Seunghan Oh, Younghoon Kim, Sungsu Park and Jong Hwan Sung
Integrative Biology, 2014, 6, 1122
DOI: 10.1039/c4ib00157e

Non-invasive sensing of transepithelial barrier function and tissue differentiation in organs-on-chips using impedance spectroscopy
Marinke W. van der Helm, Olivier Y. F. Henry, Amir Bein, Tiama Hamkins-Indik, Michael J. Cronce, William D. Leineweber, Mathieu Odijk, Andries D. van der Meer, Jan C. T. Eijkel, Donald E. Ingber, Albert van den Berg and Loes I. Segerink
Lab Chip, 2019, 19, 452
DOI: 10.1039/C8LC00129D

Smart material platforms for miniaturized devices: implications in disease models and diagnostics
Ritika Verma, Rishi Rajat Adhikary and Rinti Banerjee
Lab Chip, 2016, 16, 1978
DOI: 10.1039/C6LC00173D

Use of porous membranes in tissue barrier and co-culture models
Henry H. Chung, Marcela Mireles, Bradley J. Kwarta and Thomas R. Gaborski
Lab Chip, 2018, 18, 1671
DOI: 10.1039/C7LC01248A

Biomimetic basement membranes: advances in materials, preparation techniques, and applications in in vitro biological models
Aoxiang Jin, Chunxiang Lu, Chuang Gao, Hao Qiao, Yi Zhang, Huazhen Liu, Wenbin Sun, Qiqi Dai and Yuanyuan Liu
Biomater. Sci., 2025, 13, 2179
DOI: 10.1039/D4BM01682C

Low-cost microphysiological systems: feasibility study of a tape-based barrier-on-chip for small intestine modeling
Thomas E. Winkler, Michael Feil, Eva F. G. J. Stronkman, Isabelle Matthiesen and Anna Herland
Lab Chip, 2020, 20, 1212
DOI: 10.1039/D0LC00009D

Membrane-based microfluidic systems for medical and biological applications
Silvia Tea Calzuola, Gwenyth Newman, Thomas Feaugas, Cécile M. Perrault, Jean-Baptiste Blondé, Emmanuel Roy, Constance Porrini, Goran M. Stojanovic and Jasmina Vidic
Lab Chip, 2024, 24, 3579
DOI: 10.1039/D4LC00251B

Laminar ventricular myocardium on a microelectrode array-based chip
Ville J. Kujala, Francesco Silvio Pasqualini, Josue A. Goss, Janna C. Nawroth and Kevin Kit Parker
J. Mater. Chem. B, 2016, 4, 3534
DOI: 10.1039/C6TB00324A

Establishment of a gut-on-a-chip device with controllable oxygen gradients to study the contribution of Bifidobacterium bifidum to inflammatory bowel disease
Jun Liu, Ronghao Lu, Xiaolin Zheng, Wensheng Hou, Xiaoying Wu, Hezhao Zhao, Guixue Wang and Tian Tian
Biomater. Sci., 2023, 11, 2504
DOI: 10.1039/D2BM01490D

Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights
Wanlin Hu, Yushen Wang, Junlei Han, Wenhong Zhang, Jun Chen, Xinyu Li and Li Wang
Biomater. Sci., 2025, 13, 1624
DOI: 10.1039/D4BM01273A

A vascularized crypt-patterned colon model for high-throughput drug screening and disease modelling
Alexander Sotra, Kimia Asadi Jozani and Boyang Zhang
Lab Chip, 2023, 23, 3370
DOI: 10.1039/D3LC00211J

In vitro generation of colonic epithelium from primary cells guided by microstructures
Yuli Wang, Asad A. Ahmad, Christopher E. Sims, Scott T. Magness and Nancy L. Allbritton
Lab Chip, 2014, 14, 1622
DOI: 10.1039/C3LC51353J

Measuring direct current trans-epithelial electrical resistance in organ-on-a-chip microsystems
Mathieu Odijk, Andries D. van der Meer, Daniel Levner, Hyun Jung Kim, Marinke W. van der Helm, Loes I. Segerink, Jean-Phillipe Frimat, Geraldine A. Hamilton, Donald E. Ingber and Albert van den Berg
Lab Chip, 2015, 15, 745
DOI: 10.1039/C4LC01219D

Multiplex recreation of human intestinal morphogenesis on a multi-well insert platform by basolateral convective flow
Hyeon Beom Chong, Jaeseung Youn, Woojung Shin, Hyun Jung Kim and Dong Sung Kim
Lab Chip, 2021, 21, 3316
DOI: 10.1039/D1LC00404B

A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents
Ilka Maschmeyer, Alexandra K. Lorenz, Katharina Schimek, Tobias Hasenberg, Anja P. Ramme, Juliane Hübner, Marcus Lindner, Christopher Drewell, Sophie Bauer, Alexander Thomas, Naomia Sisoli Sambo, Frank Sonntag, Roland Lauster and Uwe Marx
Lab Chip, 2015, 15, 2688
DOI: 10.1039/C5LC00392J

A simple three-dimensional gut model constructed in a restricted ductal microspace induces intestinal epithelial cell integrity and facilitates absorption assays
Tadaaki Nakajima, Katsunori Sasaki, Akihiro Yamamori, Kengo Sakurai, Kaori Miyata, Tomoyuki Watanabe and Yukiko T. Matsunaga
Biomater. Sci., 2020, 8, 5615
DOI: 10.1039/D0BM00763C

Measuring barrier function in organ-on-chips with cleanroom-free integration of multiplexable electrodes
Elsbeth G. B. M. Bossink, Mariia Zakharova, Douwe S. de Bruijn, Mathieu Odijk and Loes I. Segerink
Lab Chip, 2021, 21, 2040
DOI: 10.1039/D0LC01289K

Advanced polymeric systems for colon drug delivery: from experimental models to market applications
R. Crispino, E. Lagreca, A. Procopio, R. D’Auria, B. Corrado, S. La Manna, V. Onesto and C. Di Natale
Soft Matter, 2025, 21, 792
DOI: 10.1039/D4SM01222D

Microphysiological systems for ADME-related applications: current status and recommendations for system development and characterization
Stephen Fowler, Wen Li Kelly Chen, David B. Duignan, Anshul Gupta, Niresh Hariparsad, Jane R. Kenny, W. George Lai, Jennifer Liras, Jonathan A. Phillips and Jinping Gan
Lab Chip, 2020, 20, 446
DOI: 10.1039/C9LC00857H

Dietary protocatechuic acid redistributes tight junction proteins by targeting Rho-associated protein kinase to improve intestinal barrier function
Bo Ram So, San Kim, Se Hyeon Jang, Min Jeong Kim, Jeong Jae Lee, Soo Rin Kim and Sung Keun Jung
Food Funct., 2023, 14, 4777
DOI: 10.1039/D3FO00605K

Steady-state monitoring of oxygen in a high-throughput organ-on-chip platform enables rapid and non-invasive assessment of drug-induced nephrotoxicity
Samuel H. Kann, Erin M. Shaughnessey, Xin Zhang, Joseph L. Charest and Else M. Vedula
Analyst, 2023, 148, 3204
DOI: 10.1039/D3AN00380A

Lab-on-chip technologies for exploring the gut–immune axis in metabolic disease
Alexandra E. Wheeler, Verena Stoeger and Róisín M. Owens
Lab Chip, 2024, 24, 1266
DOI: 10.1039/D3LC00877K

Tissue chips – innovative tools for drug development and disease modeling
L. A. Low and D. A. Tagle
Lab Chip, 2017, 17, 3026
DOI: 10.1039/C7LC00462A

Gut-on-a-chip: Current progress and future opportunities
Nureddin Ashammakhi, Rohollah Nasiri, Natan Roberto de Barros, Peyton Tebon, Jai Thakor, Marcus Goudie, Amir Shamloo, Martin G. Martin and Ali Khademhosseini
Biomaterials, 2020, 255, 120196
DOI: 10.1016/j.biomaterials.2020.120196

Organs on chips: fundamentals, bioengineering and applications
Nasser K. Awad
J Artif Organs, 2025, 28, 110
DOI: 10.1007/s10047-024-01460-0

Enterocyte Culture on a Hybrid Transwell-Inserted Gut-on-a-Chip for Liquid–Liquid and Air–Liquid Interface Conditions
Tidaporn Charoensri, Kornphimol Kulthong, Pareesa Pormrungruang, Ratjika Wongwanakul, Chanikarn Kunyanee, Sasitorn Aueviriyavit and Sakon Rahong
ACS Omega, 2025, 10, 53865
DOI: 10.1021/acsomega.5c03215

Numerical models for organ-on-a-chip: A systematic review and analyses
Weiguang Su, Yang Zhao, Siegfried Yeboah, Xinyu Li and Li Wang
Biomicrofluidics, 2025, 19
DOI: 10.1063/5.0260477

Microfluidics on lensless, semiconductor optical image sensors: challenges and opportunities for democratization of biosensing at the micro‐and nano‐scale
Xinyue Hu, Reza Abbasi and Sebastian Wachsmann‐Hogiu
Nanophotonics, 2023, 12, 3977
DOI: 10.1515/nanoph-2023-0301

A Simple, Efficient Technique to Make Colon Crypt Gels for 3D Models
Maria A. Bieberich, Narendra Pandala, Ishanathan Guteng, Victor Kehinde, Ezinne Oguguo, Rebecca Carrier and Erin Lavik
3D Printing and Additive Manufacturing, 2026
DOI: 10.1177/23297662251389590

A complex human gut microbiome cultured in an anaerobic intestine-on-a-chip
Sasan Jalili-Firoozinezhad, Francesca S. Gazzaniga, Elizabeth L. Calamari, Diogo M. Camacho, Cicely W. Fadel, Amir Bein, Ben Swenor, Bret Nestor, Michael J. Cronce, Alessio Tovaglieri, Oren Levy, Katherine E. Gregory, David T. Breault, Joaquim M. S. Cabral, Dennis L. Kasper, Richard Novak and Donald E. Ingber
Nat Biomed Eng, 2019, 3, 520
DOI: 10.1038/s41551-019-0397-0

Pharmacokinetic and pharmacodynamic insights from microfluidic intestine-on-a-chip models
Seung Hwan Lee, Nakwon Choi and Jong Hwan Sung
Expert Opinion on Drug Metabolism & Toxicology, 2019, 15, 1005
DOI: 10.1080/17425255.2019.1700950

Emerging trends in the methodology of environmental toxicology: 3D cell culture and its applications
Huan Wang, Ting Xu and Daqiang Yin
Science of The Total Environment, 2023, 857, 159501
DOI: 10.1016/j.scitotenv.2022.159501

A multi-chamber microfluidic intestinal barrier model using Caco-2 cells for drug transport studies
Hsih-Yin Tan, Sofie Trier, Ulrik L. Rahbek, Martin Dufva, Jörg P. Kutter, Thomas L. Andresen and Mária A. Deli
PLoS ONE, 2018, 13, e0197101
DOI: 10.1371/journal.pone.0197101

Validation of a Caco-2 microfluidic Chip model for predicting intestinal absorption of BCS Class I-IV drugs
Stephanie Y. Zhang, Whitney S.Y. Ong, Natalia Subelzu and John P. Gleeson
International Journal of Pharmaceutics, 2024, 656, 124089
DOI: 10.1016/j.ijpharm.2024.124089

Biochip Systems for Intelligence and Integration
Junhao Wang, Bihao Sun and Zhiyuan Zhu
Systems, 2023, 11, 43
DOI: 10.3390/systems11010043

Recent Advances in Regenerative Tissue Fabrication: Tools, Materials, and Microenvironment in Hierarchical Aspects
Monica Cahyaning Ratri, Albertus Ivan Brilian, Agustina Setiawati, Huong Thanh Nguyen, Veasna Soum and Kwanwoo Shin
Advanced NanoBiomed Research, 2021, 1
DOI: 10.1002/anbr.202000088

Organ-on-Chip Approaches for Intestinal 3D In Vitro Modeling
Joana Pimenta, Ricardo Ribeiro, Raquel Almeida, Pedro F. Costa, Marta A. da Silva and Bruno Pereira
Cellular and Molecular Gastroenterology and Hepatology, 2022, 13, 351
DOI: 10.1016/j.jcmgh.2021.08.015

A microfluidic cell culture device (μFCCD) to culture epithelial cells with physiological and morphological properties that mimic those of the human intestine
Meiying Chi, Banya Yi, Seunghan Oh, Dong-June Park, Jong Hwan Sung and Sungsu Park
Biomed Microdevices, 2015, 17
DOI: 10.1007/s10544-015-9966-5

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials
Pengchao Zhang, Ning Shao and Lidong Qin
Advanced Materials, 2021, 33
DOI: 10.1002/adma.202005944

Integrated technologies for continuous monitoring of organs-on-chips: Current challenges and potential solutions
Jonathan Sabaté del Río, Jooyoung Ro, Heejeong Yoon, Tae-Eun Park and Yoon-Kyoung Cho
Biosensors and Bioelectronics, 2023, 224, 115057
DOI: 10.1016/j.bios.2022.115057

Microfluidic chip for culturing intestinal epithelial cell layers: Characterization and comparison of drug transport between dynamic and static models
Kornphimol Kulthong, Loes Duivenvoorde, Huiyi Sun, Samuel Confederat, Jiaqing Wu, Bert Spenkelink, Laura de Haan, Victor Marin, Meike van der Zande and Hans Bouwmeester
Toxicology in Vitro, 2020, 65, 104815
DOI: 10.1016/j.tiv.2020.104815

Modeling Pharmacokinetic Profiles for Assessment of Anti-Cancer Drug on a Microfluidic System
Yaqiong Guo, Pengwei Deng, Wenwen Chen and Zhongyu Li
Micromachines, 2020, 11, 551
DOI: 10.3390/mi11060551

Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip
Neda Azizipour, Rahi Avazpour, Derek H. Rosenzweig, Mohamad Sawan and Abdellah Ajji
Micromachines, 2020, 11, 599
DOI: 10.3390/mi11060599

A Synthetic Hydrogel, VitroGel® ORGANOID-3, Improves Immune Cell-Epithelial Interactions in a Tissue Chip Co-Culture Model of Human Gastric Organoids and Dendritic Cells
Michelle D. Cherne, Barkan Sidar, T. Andrew Sebrell, Humberto S. Sanchez, Kody Heaton, Francis J. Kassama, Mandi M. Roe, Andrew B. Gentry, Connie B. Chang, Seth T. Walk, Mark Jutila, James N. Wilking and Diane Bimczok
Front. Pharmacol., 2021, 12
DOI: 10.3389/fphar.2021.707891

Drug discovery for remyelination and treatment of MS
Katy L. H. Cole, Jason J. Early and David A. Lyons
Glia, 2017, 65, 1565
DOI: 10.1002/glia.23166

Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids
Magdalena Kasendra, Alessio Tovaglieri, Alexandra Sontheimer-Phelps, Sasan Jalili-Firoozinezhad, Amir Bein, Angeliki Chalkiadaki, William Scholl, Cheng Zhang, Hannah Rickner, Camilla A. Richmond, Hu Li, David T. Breault and Donald E. Ingber
Sci Rep, 2018, 8
DOI: 10.1038/s41598-018-21201-7

Robust Formation of an Epithelial Layer of Human Intestinal Organoids in a Polydimethylsiloxane-Based Gut-on-a-Chip Microdevice
Woojung Shin, Yoko M. Ambrosini, Yong Cheol Shin, Alexander Wu, Soyoun Min, Domin Koh, Sowon Park, Seung Kim, Hong Koh and Hyun Jung Kim
Front. Med. Technol., 2020, 2
DOI: 10.3389/fmedt.2020.00002

A Pumpless and Tubeless Microfluidic Device Enables Extended In Vitro Development of Cryptosporidium parvum
Samantha Gunasekera, Benjamin Thierry, Edward Cheah, Brendon King, Paul Monis, Jillian M Carr, Abha Chopra, Mark Watson, Mark O’Dea and Una Ryan
Open Forum Infectious Diseases, 2024, 11
DOI: 10.1093/ofid/ofae625

vasQchip: A Novel Microfluidic, Artificial Blood Vessel Scaffold for Vascularized 3D Tissues
Vanessa Kappings, Christoph Grün, Darja Ivannikov, Isabella Hebeiss, Saskia Kattge, Ina Wendland, Bastian E. Rapp, Matthias Hettel, Olaf Deutschmann and Ute Schepers
Adv Materials Technologies, 2018, 3
DOI: 10.1002/admt.201700246

Organ‐on‐chip to investigate host‐pathogens interactions
Thomas Feaugas and Nathalie Sauvonnet
Cellular Microbiology, 2021, 23
DOI: 10.1111/cmi.13336

Relevance of organ(s)-on-a-chip systems to the investigation of food-gut microbiota-host interactions
Enriqueta Garcia-Gutierrez and Paul D. Cotter
Critical Reviews in Microbiology, 2022, 48, 463
DOI: 10.1080/1040841X.2021.1979933

Microbiome and In Vitro Gut Models
D. M. Hushpulian, S. V. Nikulin, A. A. Zakharyants, S. S. Savin and V. I. Tishkov
Appl Biochem Microbiol, 2024, 60, 1694
DOI: 10.1134/S0003683824700261

Microfluidic Device to Manipulate 3D Human Epithelial Cell-Derived Intestinal Organoids
Miki Matsumoto, Yuya Morimoto, Toshiro Sato and Shoji Takeuchi
Micromachines, 2022, 13, 2082
DOI: 10.3390/mi13122082

In vitro-ex vivo model systems for nanosafety assessment
Peter Wick, Savvina Chortarea, Olivier T. Guenat, Matthias Roesslein, Janick D. Stucki, Stephanie Hirn, Alke Petri-Fink and Barbara Rothen-Rutishauser
European Journal of Nanomedicine, 2015, 7, 169
DOI: 10.1515/ejnm-2014-0049

Visualization of Bacterial Colonization and Cellular Layers in a Gut-on-a-Chip System Using Optical Coherence Tomography
Lu Yuan, Pim de Haan, Brandon W. Peterson, Ed D. de Jong, Elisabeth Verpoorte, Henny C. van der Mei and Henk J. Busscher
Microsc Microanal, 2020, 26, 1211
DOI: 10.1017/S143192762002454X

Measurement of oxygen consumption rates of human renal proximal tubule cells in an array of organ-on-chip devices to monitor drug-induced metabolic shifts
Samuel H. Kann, Erin M. Shaughnessey, Jonathan R. Coppeta, Hesham Azizgolshani, Brett C. Isenberg, Else M. Vedula, Xin Zhang and Joseph L. Charest
Microsyst Nanoeng, 2022, 8
DOI: 10.1038/s41378-022-00442-7

Rapid Prototyping of Multilayer Microphysiological Systems
Sanjin Hosic, Adam J. Bindas, Marissa L. Puzan, Will Lake, Jonathan R. Soucy, Fanny Zhou, Ryan A. Koppes, David T. Breault, Shashi K. Murthy and Abigail N. Koppes
ACS Biomater. Sci. Eng., 2021, 7, 2949
DOI: 10.1021/acsbiomaterials.0c00190

Human organs-on-chips for disease modelling, drug development and personalized medicine
Donald E. Ingber
Nat Rev Genet, 2022, 23, 467
DOI: 10.1038/s41576-022-00466-9

Challenges in Permeability Assessment for Oral Drug Product Development
Mirko Koziolek, Patrick Augustijns, Constantin Berger, Rodrigo Cristofoletti, David Dahlgren, Janneke Keemink, Pär Matsson, Fiona McCartney, Marco Metzger, Mario Mezler, Janis Niessen, James E. Polli, Maria Vertzoni, Werner Weitschies and Jennifer Dressman
Pharmaceutics, 2023, 15, 2397
DOI: 10.3390/pharmaceutics15102397

Dissecting Gut‐Microbial Community Interactions using a Gut Microbiome‐on‐a‐Chip
Jeeyeon Lee, Nishanth Menon and Chwee Teck Lim
Advanced Science, 2024, 11
DOI: 10.1002/advs.202302113

Confocal Laser Scanning Microscopy-Compatible Microfluidic Membrane Flow Cell as a Nondestructive Tool for Studying Biofouling Dynamics on Forward Osmosis Membranes
Manisha Mukherjee, Nishanth V. Menon, Xin Liu, Yuejun Kang and Bin Cao
Environ. Sci. Technol. Lett., 2016, 3, 303
DOI: 10.1021/acs.estlett.6b00218

A review on gut microbiota and miRNA crosstalk: implications for Alzheimer’s disease
Maruthu Pandian Ayyanar and Murali Vijayan
GeroScience, 2024, 47, 339
DOI: 10.1007/s11357-024-01432-5

Microfluidic-Based Cell-Embedded Microgels Using Nonfluorinated Oil as a Model for the Gastrointestinal Niche
Seyed Ramin Pajoumshariati, Morteza Azizi, Daniel Wesner, Paula G. Miller, Michael L. Shuler and Alireza Abbaspourrad
ACS Appl. Mater. Interfaces, 2018, 10, 9235
DOI: 10.1021/acsami.7b16916

The epigenetic landscape in intestinal stem cells and its deregulation in colorectal cancer
Axelle E M Larue and Yaser Atlasi
Stem Cells, 2024, 42, 509
DOI: 10.1093/stmcls/sxae027

Mechanostimulatory Cues Determine Intestinal Fibroblast Fate and Profibrotic Remodeling in a Physiodynamic Human Gut‐on‐a‐Chip
Soyoun Min, Nam Than, Yong Cheol Shin, Elif G. Ertugral, Chandrasekhar R. Kothapalli, Olumuyiwa Awoniyi and Hyun Jung Kim
Advanced Science, 2026
DOI: 10.1002/advs.202516040

Integration Concepts for Multi-Organ Chips: How to Maintain Flexibility?!
Julia Rogal, Christopher Probst and Peter Loskill
Future Sci. OA, 2017, 3
DOI: 10.4155/fsoa-2016-0092

Microfluidic In Vitro Platform for (Nano)Safety and (Nano)Drug Efficiency Screening
Yvonne Kohl, Margit Biehl, Sarah Spring, Michelle Hesler, Vladimir Ogourtsov, Miomir Todorovic, Joshua Owen, Elisabeth Elje, Kristina Kopecka, Oscar Hernando Moriones, Neus G. Bastús, Peter Simon, Tibor Dubaj, Elise Rundén‐Pran, Victor Puntes, Nicola William, Hagen von Briesen, Sylvia Wagner, Nikil Kapur, Espen Mariussen, Andrew Nelson, Alena Gabelova, Maria Dusinska, Thomas Velten and Thorsten Knoll
Small, 2021, 17
DOI: 10.1002/smll.202006012

Does flow culture impact upon gut-probiotic interactions: A comparison with static culture
D.R. Michael, J. Kerry-Smith, T.S. Webberley, K.R. Murphy, S.F. Plummer, L. Parry and J.R. Marchesi
Journal of Functional Foods, 2023, 104, 105519
DOI: 10.1016/j.jff.2023.105519

Microfluidic organ chip of fluid–solid dynamic curved interface
Haoran Su, Tianxiang Ma, Xiao Liu, Li Wang, Fangjun Shu, Zhuqing Liang, Dongrui Zhang, Xing Zhang, Kexin Li, Min Wang, Chen Xin, Yu Zhang, Jing Zhang, Yao Du and Yubo Fan
Applied Physics Reviews, 2024, 11
DOI: 10.1063/5.0177386

Recent lab‐on‐chip developments for novel drug discovery
Nauman Khalid, Isao Kobayashi and Mitsutoshi Nakajima
WIREs Mechanisms of Disease, 2017, 9
DOI: 10.1002/wsbm.1381

Celiac disease‐on‐chip: Modeling a multifactorial disease in vitro
Renée Moerkens, Joram Mooiweer, Sebo Withoff and Cisca Wijmenga
UEG Journal, 2019, 7, 467
DOI: 10.1177/2050640619836057

Genotoxicity of Nanomaterials: Advanced In Vitro Models and High Throughput Methods for Human Hazard Assessment—A Review
Yvonne Kohl, Elise Rundén-Pran, Espen Mariussen, Michelle Hesler, Naouale El Yamani, Eleonora Marta Longhin and Maria Dusinska
Nanomaterials, 2020, 10, 1911
DOI: 10.3390/nano10101911

Synthetic Biology-Based Approaches to Investigate Host–Pathogen Interactions
Rajdeep Banerjee
SynBio, 2025, 3, 4
DOI: 10.3390/synbio3010004

Liver and kidney cells cultures in a new perfluoropolyether biochip
Rachid Jellali, Patrick Paullier, Marie-José Fleury and Eric Leclerc
Sensors and Actuators B: Chemical, 2016, 229, 396
DOI: 10.1016/j.snb.2016.01.141

From animal models to gut-on-chip: the challenging journey to capture inter-individual variability in chronic digestive disorders
Aicha Kriaa, Vincent Mariaule, Charlotte De Rudder, Amin Jablaoui, Harry Sokol, Paul Wilmes, Emmanuelle Maguin and Moez Rhimi
Gut Microbes, 2024, 16
DOI: 10.1080/19490976.2024.2333434

Gut-on-a-chip models for dissecting the gut microbiology and physiology
Amin Valiei, Javad Aminian-Dehkordi and Mohammad R. K. Mofrad
APL Bioengineering, 2023, 7
DOI: 10.1063/5.0126541

Microfluidic intestine-on-a-chip: Current progress and further perspectives of probiotic-foodborne pathogen interactions
Xinran Xiang, Xinbin Wang, Yuting Shang and Yu Ding
Trends in Food Science & Technology, 2023, 134, 207
DOI: 10.1016/j.tifs.2023.03.006

Effect of flow and peristaltic mixing on bacterial growth in a gut-like channel
Jonas Cremer, Igor Segota, Chih-yu Yang, Markus Arnoldini, John T. Sauls, Zhongge Zhang, Edgar Gutierrez, Alex Groisman and Terence Hwa
Proc. Natl. Acad. Sci. U.S.A., 2016, 113, 11414
DOI: 10.1073/pnas.1601306113

Organ-on-a-Chip Applications in Microfluidic Platforms
Ling An, Yi Liu and Yaling Liu
Micromachines, 2025, 16, 201
DOI: 10.3390/mi16020201

Microfluidic organ-on-chip technology for blood-brain barrier research
Marinke W van der Helm, Andries D van der Meer, Jan C T Eijkel, Albert van den Berg and Loes I Segerink
Tissue Barriers, 2016, 4, e1142493
DOI: 10.1080/21688370.2016.1142493

Translating advances in organ‐on‐a‐chip technology for supporting organs
Nureddin Ashammakhi, Elmahdi Elkhammas and Anwarul Hasan
J Biomed Mater Res, 2019, 107, 2006
DOI: 10.1002/jbm.b.34292

Recent advances in in vitro skin-on-a-chip models for drug testing
Jong Hwan Sung and Jae Jung Kim
Expert Opinion on Drug Metabolism & Toxicology, 2023, 19, 249
DOI: 10.1080/17425255.2023.2227379

Microfabrication of human organs-on-chips
Dongeun Huh, Hyun Jung Kim, Jacob P Fraser, Daniel E Shea, Mohammed Khan, Anthony Bahinski, Geraldine A Hamilton and Donald E Ingber
Nat Protoc, 2013, 8, 2135
DOI: 10.1038/nprot.2013.137

Evaluating Human Intestinal Cell Lines for Studying Dietary Protein Absorption
Paulus Jochems, Johan Garssen, Antonius Van Keulen, Rosalinde Masereeuw and Prescilla Jeurink
Nutrients, 2018, 10, 322
DOI: 10.3390/nu10030322

Gastrointestinal tract, its pathophysiology and in-vitro models: A “quick” reference guide to translational studies
Kristijan Skok, Boštjan Vihar, Uroš Maver, Lidija Gradišnik, Konstantin Bräutigam, Martin Trapecar and Pavel Skok
World J Gastroenterol, 2025, 31
DOI: 10.3748/wjg.v31.i28.108297

Safety concerns over the use of intestinal permeation enhancers: A mini-review
Fiona McCartney, John P. Gleeson and David J. Brayden
Tissue Barriers, 2016, 4, e1176822
DOI: 10.1080/21688370.2016.1176822

Microphysiological Systems to Recapitulate the Gut–Kidney Axis
Laura Giordano, Silvia Maria Mihaila, Hossein Eslami Amirabadi and Rosalinde Masereeuw
Trends in Biotechnology, 2021, 39, 811
DOI: 10.1016/j.tibtech.2020.12.001

Engineering vascularized inner ear organoids: Challenges and advances in recapitulating the cochlear microenvironment
Hao Rong, Qian Zhu, Zhonghong Zhang, Xinyi Pang, Jiyang Zuo, Yusong Wang, Pan Feng, Xu Zhang, Liuying Gao, Lei Tian, Yangnan Hu, Zhisen Shen and Renjie Chai
Biomaterials, 2026, 329, 123909
DOI: 10.1016/j.biomaterials.2025.123909

Integration of Extracellular Matrices into Organ‐on‐Chip Systems
Hazal Kutluk, Effie E. Bastounis and Iordania Constantinou
Adv Healthcare Materials, 2023, 12
DOI: 10.1002/adhm.202203256

Advances in bio-inspired electrocatalysts for clean energy future
Jing-Wen DuanMu and Min-Rui Gao
Nano Res., 2024, 17, 515
DOI: 10.1007/s12274-023-5977-3

Intestinal barrier dysfunction orchestrates the onset of inflammatory host–microbiome cross-talk in a human gut inflammation-on-a-chip
Woojung Shin and Hyun Jung Kim
Proc. Natl. Acad. Sci. U.S.A., 2018, 115
DOI: 10.1073/pnas.1810819115

Analytical and biomedical applications of microfluidics in traditional Chinese medicine research
Peiliang Shen, Yuanyuan Jia, Silin Shi, Jia Sun and Xin Han
TrAC Trends in Analytical Chemistry, 2023, 158, 116851
DOI: 10.1016/j.trac.2022.116851

Strengths and limitations of in vitro and animal models to advance understanding of human diet‒microbiome interactions
Alexa R. Weingarden
Gut Microbes Reports, 2026, 3
DOI: 10.1080/29933935.2026.2636336

Potential Application of Living Microorganisms in the Detoxification of Heavy Metals
Runqiu Chen, Huaijun Tu and Tingtao Chen
Foods, 2022, 11, 1905
DOI: 10.3390/foods11131905

Peripheral nerve sheath tumors-on-a-chip: Next-generation platforms for mechanistic and therapeutic studies
William H. Delaney, Sara A. Faulkner, Wilbur A. Lam, Nicholas M. Boulis, Yongzhi Qiu and Kecheng Lei
Materials Today Advances, 2026, 30, 100770
DOI: 10.1016/j.mtadv.2026.100770

Organ-on-a-chip platforms for evaluation of environmental nanoparticle toxicity
Rick Xing Ze Lu and Milica Radisic
Bioactive Materials, 2021, 6, 2801
DOI: 10.1016/j.bioactmat.2021.01.021

Magnetically-propelled fecal surrogates for modeling the impact of solid-induced shear forces on primary colonic epithelial cells
Samuel S. Hinman, Jennifer Huling, Yuli Wang, Hao Wang, Ross C. Bretherton, Cole A. DeForest and Nancy L. Allbritton
Biomaterials, 2021, 276, 121059
DOI: 10.1016/j.biomaterials.2021.121059

Incorporating mechanical strain in organs-on-a-chip: Lung and skin
Olivier T. Guenat and François Berthiaume
Biomicrofluidics, 2018, 12
DOI: 10.1063/1.5024895

Comparison of conventional and advancedin vitromodels in the toxicity testing of nanoparticles
Eleonore Fröhlich
Artificial Cells, Nanomedicine, and Biotechnology, 2018, 46, 1091
DOI: 10.1080/21691401.2018.1479709

Microfluidics as a Novel Tool for Biological and Toxicological Assays in Drug Discovery Processes: Focus on Microchip Electrophoresis
Giuseppe Caruso, Nicolò Musso, Margherita Grasso, Angelita Costantino, Giuseppe Lazzarino, Fabio Tascedda, Massimo Gulisano, Susan M. Lunte and Filippo Caraci
Micromachines, 2020, 11, 593
DOI: 10.3390/mi11060593

Direct therapeutic effect of sulfadoxine-pyrimethamine on nutritional deficiency-induced enteric dysfunction in a human Intestine Chip
Seongmin Kim, Arash Naziripour, Pranav Prabhala, Viktor Horváth, Abidemi Junaid, David T. Breault, Girija Goyal and Donald E. Ingber
eBioMedicine, 2024, 99, 104921
DOI: 10.1016/j.ebiom.2023.104921

Advances in modelling the human microbiome–gut–brain axis in vitro
Chrysanthi-Maria Moysidou and Róisín M. Owens
Biochemical Society Transactions, 2021, 49, 187
DOI: 10.1042/BST20200338

In vitro models to evaluate ingestible devices: Present status and current trends
Connor O'Farrell, Konstantinos Stamatopoulos, Mark Simmons and Hannah Batchelor
Advanced Drug Delivery Reviews, 2021, 178, 113924
DOI: 10.1016/j.addr.2021.113924

3D In Vitro Human Organ Mimicry Devices for Drug Discovery, Development, and Assessment
Aida Rodriguez-Garcia, Jacqueline Oliva-Ramirez, Claudia Bautista-Flores and Samira Hosseini
Advances in Polymer Technology, 2020, 2020, 1
DOI: 10.1155/2020/6187048

Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies?
Donald E. Ingber
Advanced Science, 2020, 7
DOI: 10.1002/advs.202002030

Flat and microstructured polymeric membranes in organs-on-chips
Thijs Pasman, Dirk Grijpma, Dimitrios Stamatialis and Andreas Poot
J. R. Soc. Interface., 2018, 15, 20180351
DOI: 10.1098/rsif.2018.0351

Microphysiological Systems: Design, Fabrication, and Applications
Kai Wang, Kun Man, Jiafeng Liu, Yang Liu, Qi Chen, Yong Zhou and Yong Yang
ACS Biomater. Sci. Eng., 2020, 6, 3231
DOI: 10.1021/acsbiomaterials.9b01667

Gut bioengineering promotes gut repair and pharmaceutical research: a review
Jinjian Huang, Yanhan Ren, Xiuwen Wu, Zongan Li and Jianan Ren
J Tissue Eng, 2019, 10
DOI: 10.1177/2041731419839846

3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert
Woojung Shin and Hyun Jung Kim
Nat Protoc, 2022, 17, 910
DOI: 10.1038/s41596-021-00674-3

Innate immune responses play a key role in controlling infection of the intestinal epithelium by Cryptosporidium
Fabrice Laurent and Sonia Lacroix-Lamandé
International Journal for Parasitology, 2017, 47, 711
DOI: 10.1016/j.ijpara.2017.08.001

Microscale technologies for regulating human stem cell differentiation
Elisa Cimetta and Gordana Vunjak-Novakovic
Exp Biol Med (Maywood), 2014, 239, 1255
DOI: 10.1177/1535370214530369

Organs-on-chips at the frontiers of drug discovery
Eric W. Esch, Anthony Bahinski and Dongeun Huh
Nat Rev Drug Discov, 2015, 14, 248
DOI: 10.1038/nrd4539

An overview of in vitro, ex vivo and in vivo models for studying the transport of drugs across intestinal barriers
Yining Xu, Neha Shrestha, Véronique Préat and Ana Beloqui
Advanced Drug Delivery Reviews, 2021, 175, 113795
DOI: 10.1016/j.addr.2021.05.005

Oral delivery of protein-based therapeutics: Gastroprotective strategies, physiological barriers and in vitro permeability prediction
Leah Wright, Timothy J. Barnes and Clive A. Prestidge
International Journal of Pharmaceutics, 2020, 585, 119488
DOI: 10.1016/j.ijpharm.2020.119488

Bioengineering Hematopoietic Stem Cell Niche toward Regenerative Medicine
Ryohichi Sugimura
Advanced Drug Delivery Reviews, 2016, 99, 212
DOI: 10.1016/j.addr.2015.10.010

Tissue Engineering in Ophthalmology: Implications for Eyelid Reconstruction
Michelle T. Sun, Andrea J. O’Connor, John Wood, Robert Casson and Dinesh Selva
Ophthalmic Plastic & Reconstructive Surgery, 2017, 33, 157
DOI: 10.1097/IOP.0000000000000792

Multi-organ-on-a-chip for pharmacokinetics and toxicokinetic study of drugs
Jong Hwan Sung
Expert Opinion on Drug Metabolism & Toxicology, 2021, 17, 969
DOI: 10.1080/17425255.2021.1908996

Cancer Modeling‐on‐a‐Chip with Future Artificial Intelligence Integration
Kirsten Lee Fetah, Benjamin J. DiPardo, Eve‐Mary Kongadzem, James S. Tomlinson, Adam Elzagheid, Mohammed Elmusrati, Ali Khademhosseini and Nureddin Ashammakhi
Small, 2019, 15
DOI: 10.1002/smll.201901985

Contributions of the microbiome to intestinal inflammation in a gut-on-a-chip
Min Seo Jeon, Yoon Young Choi, Sung Jun Mo, Jang Ho Ha, Young Seo Lee, Hee Uk Lee, Soo Dong Park, Jae-Jung Shim, Jung-Lyoul Lee and Bong Geun Chung
Nano Convergence, 2022, 9
DOI: 10.1186/s40580-022-00299-6

Engineering and monitoring cellular barrier models
Jose Yeste, Xavi Illa, Mar Alvarez and Rosa Villa
J Biol Eng, 2018, 12
DOI: 10.1186/s13036-018-0108-5

Gut-on-a-Chip for In Vitro Drug Absorption Models
Seung Yeon Lee and Jong Hwan Sung
BioChip J, 2025, 19, 545
DOI: 10.1007/s13206-025-00205-2

Dentin on the nanoscale: Hierarchical organization, mechanical behavior and bioinspired engineering
Luiz E. Bertassoni
Dental Materials, 2017, 33, 637
DOI: 10.1016/j.dental.2017.03.008

From mechanobiology to developmentally inspired engineering
Donald E. Ingber
Phil. Trans. R. Soc. B, 2018, 373, 20170323
DOI: 10.1098/rstb.2017.0323

Engineering biomimetic intestinal topological features in 3D tissue models: retrospects and prospects
Tarun Agarwal, Valentina Onesto, Lallepak Lamboni, Aafreen Ansari, Tapas K. Maiti, Pooyan Makvandi, Massoud Vosough and Guang Yang
Bio-des. Manuf., 2021, 4, 568
DOI: 10.1007/s42242-020-00120-5

Versatile membrane-based microfluidic platform for in vitro drug diffusion testing mimicking in vivo environments
Oihane Mitxelena-Iribarren, Claudia Olaizola, Sergio Arana and Maite Mujika
Nanomedicine: Nanotechnology, Biology and Medicine, 2022, 39, 102462
DOI: 10.1016/j.nano.2021.102462

Demonstration of Interposed Modular Hydrogel Sheet for Multicellular Analysis in a Microfluidic Assembly Platform
Chae Yun Bae, Jaejung Son, Hail Kim and Je-Kyun Park
Sci Rep, 2017, 7
DOI: 10.1038/s41598-017-01363-6

3D functional and perfusable microvascular networks for organotypic microfluidic models
Simone Bersini and Matteo Moretti
J Mater Sci: Mater Med, 2015, 26
DOI: 10.1007/s10856-015-5520-5

Centrifugal Microfluidic Cell Culture Platform for Physiologically Relevant Virus Infection Studies: A Case Study with HSV-1 Infection of Periodontal Cells
Juliane Fjelrad Christfort, Morgane Ortis, Hau Van Nguyen, Robert Marsault and Alain Doglio
Biosensors, 2024, 14, 401
DOI: 10.3390/bios14080401

Microfluidics: Insights into Intestinal Microorganisms
Ping Qi, Jin Lv, Xiangdong Yan, Liuhui Bai and Lei Zhang
Microorganisms, 2023, 11, 1134
DOI: 10.3390/microorganisms11051134

Tools for probing host-bacteria interactions in the gut microenvironment: From molecular to cellular levels
Kimberly A. Wodzanowski, Samantha E. Cassel, Catherine L. Grimes and April M. Kloxin
Bioorganic & Medicinal Chemistry Letters, 2020, 30, 127116
DOI: 10.1016/j.bmcl.2020.127116

“Mucus-on-Chip”: A new tool to study the dynamic penetration of nanoparticulate drug carriers into mucus
Zhengyang Jia, Zhaobin Guo, Chih-Tsung Yang, Clive Prestidge and Benjamin Thierry
International Journal of Pharmaceutics, 2021, 598, 120391
DOI: 10.1016/j.ijpharm.2021.120391

Microbiota and cancer: In vitro and in vivo models to evaluate nanomedicines
Abdallah Ladaycia, Brigitta Loretz, Catherine Passirani, Claus-Michael Lehr and Elise Lepeltier
Advanced Drug Delivery Reviews, 2021, 170, 44
DOI: 10.1016/j.addr.2020.12.015

A 3D Toolbox to Enhance Physiological Relevance of Human Tissue Models
Nathalie Picollet-D’hahan, Monika E. Dolega, Lavinia Liguori, Christophe Marquette, Séverine Le Gac, Xavier Gidrol and Donald K. Martin
Trends in Biotechnology, 2016, 34, 757
DOI: 10.1016/j.tibtech.2016.06.012

A Theoretical and Experimental Study to Optimize Cell Differentiation in a Novel Intestinal Chip
Nicky Langerak, Haysam M. M. Ahmed, Yang Li, Igor R. Middel, Hossein Eslami Amirabadi, Jos Malda, Rosalinde Masereeuw and René van Roij
Front. Bioeng. Biotechnol., 2020, 8
DOI: 10.3389/fbioe.2020.00763

MCF-7 Human Breast Cancer Cells Form Differentiated Microtissues in Scaffold-Free Hydrogels
Marguerite M. Vantangoli, Samantha J. Madnick, Susan M. Huse, Paula Weston, Kim Boekelheide and Robert Oshima
PLoS ONE, 2015, 10, e0135426
DOI: 10.1371/journal.pone.0135426

Development of an In Vitro Airway Epithelial–Endothelial Cell Culture Model on a Flexible Porous Poly(Trimethylene Carbonate) Membrane Based on Calu-3 Airway Epithelial Cells and Lung Microvascular Endothelial Cells
Thijs Pasman, Danielle Baptista, Sander van Riet, Roman K. Truckenmüller, Pieter S. Hiemstra, Robbert J. Rottier, Naomi M. Hamelmann, Jos M. J. Paulusse, Dimitrios Stamatialis and André A. Poot
Membranes, 2021, 11, 197
DOI: 10.3390/membranes11030197

A novel membrane-on-chip guides morphogenesis for the reconstruction of the intestinal crypt-villus axis
Sara Sibilio, Raffaele Mennella, Vincenza De Gregorio, Alessia La Rocca, Francesco Urciuolo, Giorgia Imparato and Paolo A Netti
Biofabrication, 2024, 16, 045019
DOI: 10.1088/1758-5090/ad6599

Basement membrane properties and their recapitulation in organ-on-chip applications
Golestan Salimbeigi, Nihal E. Vrana, Amir M. Ghaemmaghami, Pinar Y. Huri and Garrett B. McGuinness
Materials Today Bio, 2022, 15, 100301
DOI: 10.1016/j.mtbio.2022.100301

Organ-on-a-Chip: New Tool for Personalized Medicine
Nureddin A. Ashammakhi and Adam Elzagheid
Journal of Craniofacial Surgery, 2018, 29, 823
DOI: 10.1097/SCS.0000000000004604

A new microfluidic method enabling the generation of multi-layered tissues-on-chips using skin cells as a proof of concept
L. Valencia, V. Canalejas-Tejero, M. Clemente, I. Fernaud, M. Holgado, J. L. Jorcano and D. Velasco
Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-91875-z

Resolving host–microbe interactions in the gut: the promise of in vitro models to complement in vivo research
Wilmes Paul, Calatayud Marta and Van de Wiele Tom
Current Opinion in Microbiology, 2018, 44, 28
DOI: 10.1016/j.mib.2018.07.001

Microphysiological systems in absorption, distribution, metabolism, and elimination sciences
Kirk P. Van Ness, Francine Cesar, Catherine K. Yeung, Jonathan Himmelfarb and Edward J. Kelly
Clinical Translational Sci, 2022, 15, 9
DOI: 10.1111/cts.13132

Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering
Ana Perestrelo, Ana Águas, Alberto Rainer and Giancarlo Forte
Sensors, 2015, 15, 31142
DOI: 10.3390/s151229848

SCRIB controls apical contractility during epithelial differentiation
Batiste Boëda, Vincent Michel, Raphael Etournay, Patrick England, Stéphane Rigaud, Héloïse Mary, Samy Gobaa and Sandrine Etienne-Manneville
Journal of Cell Biology, 2023, 222
DOI: 10.1083/jcb.202211113

Organ-on-chip models for infectious disease research
Raquel Alonso-Roman, Alexander S. Mosig, Marc Thilo Figge, Kai Papenfort, Christian Eggeling, Felix H. Schacher, Bernhard Hube and Mark S. Gresnigt
Nat Microbiol, 2024, 9, 891
DOI: 10.1038/s41564-024-01645-6

Organ‐on‐a‐Chip Systems for Women's Health Applications
Janna Nawroth, Julia Rogal, Martin Weiss, Sara Y. Brucker and Peter Loskill
Adv Healthcare Materials, 2018, 7
DOI: 10.1002/adhm.201700550

Gut-on-chip: Recreating human intestine in vitro
Yunqing Xiang, Hui Wen, Yue Yu, Mingqiang Li, Xiongfei Fu and Shuqiang Huang
J Tissue Eng, 2020, 11
DOI: 10.1177/2041731420965318

Reproducibility: Respect your cells!
Monya Baker
Nature, 2016, 537, 433
DOI: 10.1038/537433a

The latest advances in high content screening in microfluidic devices
Weiyu Liu, Jingyu Wang, Huibo Qi, Qisen Jiao, Lei Wu, Yu Wang and Qionglin Liang
Expert Opinion on Drug Discovery, 2023, 18, 781
DOI: 10.1080/17460441.2023.2216013

A Polymer Chemistry Point of View on Mucoadhesion and Mucopenetration
Philipp Schattling, Essi Taipaleenmäki, Yan Zhang and Brigitte Städler
Macromolecular Bioscience, 2017, 17
DOI: 10.1002/mabi.201700060

Regional Diversity of the Gastrointestinal Microbiome
Kristina Martinez-Guryn, Vanessa Leone and Eugene B. Chang
Cell Host & Microbe, 2019, 26, 314
DOI: 10.1016/j.chom.2019.08.011

Organoid-based Models to Study the Role of Host-microbiota Interactions in IBD
Martina Poletti, Kaline Arnauts, Marc Ferrante and Tamas Korcsmaros
Journal of Crohn's and Colitis, 2021, 15, 1222
DOI: 10.1093/ecco-jcc/jjaa257

Cellular Biomechanics in Drug Screening and Evaluation: Mechanopharmacology
Ramaswamy Krishnan, Jin-Ah Park, Chun Y. Seow, Peter V-S. Lee and Alastair G. Stewart
Trends in Pharmacological Sciences, 2016, 37, 87
DOI: 10.1016/j.tips.2015.10.005

In vitroinfection models to study fungal–host interactions
Antonia Last, Michelle Maurer, Alexander S. Mosig, Mark S. Gresnigt and Bernhard Hube
FEMS Microbiology Reviews, 2021, 45
DOI: 10.1093/femsre/fuab005

Gut-on-a-Chip-Based Real-Time miRNA-21 Monitoring and Anti-Inflammatory Drug Evaluation
Zhipeng Xu, Jiemeng Ding, Qi Meng, Ke Wang, Huimin Li, Fanwei Meng, Junlei Han and Li Wang
ACS Sens., 2025, 10, 8297
DOI: 10.1021/acssensors.5c00957

Optimization of 3-D organotypic primary colonic cultures for organ-on-chip applications
Asad A Ahmad, Yuli Wang, Adam D Gracz, Christopher E Sims, Scott T Magness and Nancy L Allbritton
J Biol Eng, 2014, 8
DOI: 10.1186/1754-1611-8-9

Synthetic Ecology of Microbes: Mathematical Models and Applications
Ali R. Zomorrodi and Daniel Segrè
Journal of Molecular Biology, 2016, 428, 837
DOI: 10.1016/j.jmb.2015.10.019

A 3D Bioprinted Gut Anaerobic Model for Studying Bacteria–Host Interactions
Liqin Cheng, Tingting Liu, Qiongg Liu, Liming Lian, Guosheng Tang, Luis Santiago Mille, Fabricio Romero García, Lars Engstrand, Yu Shrike Zhang and Juan Du
Research, 2023, 6
DOI: 10.34133/research.0058

Oral absorption of peptides and nanoparticles across the human intestine: Opportunities, limitations and studies in human tissues
P. Lundquist and P. Artursson
Advanced Drug Delivery Reviews, 2016, 106, 256
DOI: 10.1016/j.addr.2016.07.007

A versatile, compartmentalised gut-on-a-chip system for pharmacological and toxicological analyses
Pim de Haan, Milou J. C. Santbergen, Meike van der Zande, Hans Bouwmeester, Michel W. F. Nielen and Elisabeth Verpoorte
Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-84187-9

The third dimension: new developments in cell culture models for colorectal research
Joana F. S. Pereira, Nikhil T. Awatade, Cláudia A. Loureiro, Paulo Matos, Margarida D. Amaral and Peter Jordan
Cell. Mol. Life Sci., 2016, 73, 3971
DOI: 10.1007/s00018-016-2258-2

Microphysiological systems to study colorectal cancer: state-of-the-art
Pedro Ramos, Mariana R Carvalho, Wei Chen, Le-Ping Yan, Chang-Hua Zhang, Yu-long He, Rui L Reis and Joaquim M Oliveira
Biofabrication, 2023, 15, 032001
DOI: 10.1088/1758-5090/acc279

A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier
Astgik Petrosyan, Paolo Cravedi, Valentina Villani, Andrea Angeletti, Joaquin Manrique, Alessandra Renieri, Roger E. De Filippo, Laura Perin and Stefano Da Sacco
Nat Commun, 2019, 10
DOI: 10.1038/s41467-019-11577-z

Bioengineered human gut-on-a-chip for advancing non-clinical pharmaco-toxicology
Yong Cheol Shin, Nam Than, Soo Jin Park and Hyun Jung Kim
Expert Opinion on Drug Metabolism & Toxicology, 2024, 20, 593
DOI: 10.1080/17425255.2024.2365254

The Contributions of Human Mini-Intestines to the Study of Intestinal Physiology and Pathophysiology
Huimin Yu, Nesrin M. Hasan, Julie G. In, Mary K. Estes, Olga Kovbasnjuk, Nicholas C. Zachos and Mark Donowitz
Annu. Rev. Physiol., 2017, 79, 291
DOI: 10.1146/annurev-physiol-021115-105211

Recapitulation of the accessible interface of biopsy-derived canine intestinal organoids to study epithelial-luminal interactions
Yoko M. Ambrosini, Yejin Park, Albert E. Jergens, Woojung Shin, Soyoun Min, Todd Atherly, Dana C. Borcherding, Jinah Jang, Karin Allenspach, Jonathan P. Mochel, Hyun Jung Kim and Mária A. Deli
PLoS ONE, 2020, 15, e0231423
DOI: 10.1371/journal.pone.0231423

A Comprehensive Review of Recent Advances in Simulated Human in-Vitro Digestion System
Sandip Sanjay Gite, Moumita Karmakar, Chandrakant Genu Dalbhagat, Shubham Mahendra Nimbkar, Vivek Kambhampati, Madhuresh Dwivedi, Rama Chandra Pradhan and Sabyasachi Mishra
Food Reviews International, 2026, 1
DOI: 10.1080/87559129.2026.2647039

Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia
Kornphimol Kulthong, Guido J. E. J. Hooiveld, Loes Duivenvoorde, Ignacio Miro Estruch, Victor Marin, Meike van der Zande and Hans Bouwmeester
Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-82853-6

Perfusable and stretchable 3D culture system for skin-equivalent
Nobuhito Mori, Yuya Morimoto and Shoji Takeuchi
Biofabrication, 2019, 11, 011001
DOI: 10.1088/1758-5090/aaed12

Biomimetic Tissue–Engineered Systems for Advancing Cancer Research: NCI Strategic Workshop Report
Teresa K. Schuessler, Xin Yi Chan, Huanhuan Joyce Chen, Kyungmin Ji, Kyung Min Park, Alireza Roshan-Ghias, Pallavi Sethi, Archana Thakur, Xi Tian, Aranzazu Villasante, Ioannis K. Zervantonakis, Nicole M. Moore, Larry A. Nagahara and Nastaran Z. Kuhn
Cancer Research, 2014, 74, 5359
DOI: 10.1158/0008-5472.CAN-14-1706

Immune Organs and Immune Cells on a Chip: An Overview of Biomedical Applications
Margaretha Morsink, Niels Willemen, Jeroen Leijten, Ruchi Bansal and Su Shin
Micromachines, 2020, 11, 849
DOI: 10.3390/mi11090849

Thin and stretchable extracellular matrix (ECM) membrane reinforced by nanofiber scaffolds for developing in vitro barrier models
Jaeseung Youn, Hyeonjun Hong, Woojung Shin, Dohui Kim, Hyun Jung Kim and Dong Sung Kim
Biofabrication, 2022, 14, 025010
DOI: 10.1088/1758-5090/ac4dd7

Engineering Microphysiological Immune System Responses on Chips
Chris P. Miller, Woojung Shin, Eun Hyun Ahn, Hyun Jung Kim and Deok-Ho Kim
Trends in Biotechnology, 2020, 38, 857
DOI: 10.1016/j.tibtech.2020.01.003

Personalized Disease Models on a Chip
Nalin Tejavibulya and Samuel K. Sia
Cell Systems, 2016, 3, 416
DOI: 10.1016/j.cels.2016.11.002

Development of a Liver-Gut device for the Evaluation of Drug Bioavailability
Takahiro Iwao and Tamihide Matsunaga
DDS, 2019, 34, 249
DOI: 10.2745/dds.34.249

Advances in cell-based permeability assays to screen drugs for intestinal absorption
Donna A. Volpe
Expert Opinion on Drug Discovery, 2020, 15, 539
DOI: 10.1080/17460441.2020.1735347

Three-Dimensional Regeneration of Patient-Derived Intestinal Organoid Epithelium in a Physiodynamic Mucosal Interface-on-a-Chip
Yong Cheol Shin, Woojung Shin, Domin Koh, Alexander Wu, Yoko M. Ambrosini, Soyoun Min, S. Gail Eckhardt, R. Y. Declan Fleming, Seung Kim, Sowon Park, Hong Koh, Tae Kyung Yoo and Hyun Jung Kim
Micromachines, 2020, 11, 663
DOI: 10.3390/mi11070663

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices
David E. Lefebvre, Koen Venema, Lourdes Gombau, Luis G. Valerio, Jayadev Raju, Genevieve S. Bondy, Hans Bouwmeester, R. Paul Singh, Amy J. Clippinger, Eva-Maria Collnot, Rekha Mehta and Vicki Stone
Nanotoxicology, 2015, 9, 523
DOI: 10.3109/17435390.2014.948091

Organoids, organs-on-chips and other systems, and microbiota
Julian R. Marchesi, Stephanie May, Samantha Evans and Lee Parry
Emerging Topics in Life Sciences, 2017, 1, 385
DOI: 10.1042/ETLS20170047

A comprehensive review on organ-on-chips as powerful preclinical models to study tissue barriers
Yagmur Filiz, Alessio Esposito, Carmelo De Maria, Giovanni Vozzi and Ozlem Yesil-Celiktas
Prog. Biomed. Eng., 2024, 6, 042001
DOI: 10.1088/2516-1091/ad776c

The Convergence of Artificial Intelligence and Microfluidics in Drug Research and Development
Du Qiao, Hongxia Li, Xue Zhang, Xuhui Chen, Jiang Zhang, Jianan Zou, Danyang Zhao, Weiping Zhu, Xuhong Qian and Honglin Li
Engineering, 2025, 55, 125
DOI: 10.1016/j.eng.2025.07.025

The Past, Present and Future of Intestinal In Vitro Cell Systems for Drug Absorption Studies
Sonia Youhanna and Volker M. Lauschke
Journal of Pharmaceutical Sciences, 2021, 110, 50
DOI: 10.1016/j.xphs.2020.07.001

Emerging microfluidic gut-on-a-chip systems for drug development
Xueqi Wang, Yuzhuo Zhu, Zhaoming Cheng, Chuanjun Zhang, Yumeng Liao, Boshi Liu, Di Zhang, Zheng Li and Yuxin Fang
Acta Biomaterialia, 2024, 188, 48
DOI: 10.1016/j.actbio.2024.09.012

Duodenum Intestine-Chip for preclinical drug assessment in a human relevant model
Magdalena Kasendra, Raymond Luc, Jianyi Yin, Dimitris V Manatakis, Gauri Kulkarni, Carolina Lucchesi, Josiah Sliz, Athanasia Apostolou, Laxmi Sunuwar, Jenifer Obrigewitch, Kyung-Jin Jang, Geraldine A Hamilton, Mark Donowitz and Katia Karalis
eLife, 2020, 9
DOI: 10.7554/eLife.50135

Advances in quantification and analysis of the celiac‐related immunogenic potential of gluten
Miguel Ribeiro, Telma de Sousa, Carolina Sabença, Patrícia Poeta, Ana Sofia Bagulho and Gilberto Igrejas
Comp Rev Food Sci Food Safe, 2021, 20, 4278
DOI: 10.1111/1541-4337.12828

Mechanical Strain-Enabled Reconstitution of Dynamic Environment in Organ-on-a-Chip Platforms: A Review
Qianbin Zhao, Tim Cole, Yuxin Zhang and Shi-Yang Tang
Micromachines, 2021, 12, 765
DOI: 10.3390/mi12070765

Emulating Host-Microbiome Ecosystem of Human Gastrointestinal Tract in Vitro
Gun-Seok Park, Min Hee Park, Woojung Shin, Connie Zhao, Sameer Sheikh, So Jung Oh and Hyun Jung Kim
Stem Cell Rev and Rep, 2017, 13, 321
DOI: 10.1007/s12015-017-9739-z

Facile fabrication of microperforated membranes with re-useable SU-8 molds for organs-on-chips
Pim de Haan, Klaus Mathwig, Lu Yuan, Brandon W. Peterson and Elisabeth Verpoorte
Organs-on-a-Chip, 2023, 5, 100026
DOI: 10.1016/j.ooc.2023.100026

PDMS-Based Microfluidic Devices for Cell Culture
Stefania Torino, Brunella Corrado, Mario Iodice and Giuseppe Coppola
Inventions, 2018, 3, 65
DOI: 10.3390/inventions3030065

Geometrically Controlled WNT Activation Drives Intestinal Morphogenesis
Nathaniel C. Burmas, Arlyn M. Fabian, Amoli Vanavadiya and Quinton Smith
Adv Healthcare Materials, 2025, 14
DOI: 10.1002/adhm.202502832

Gut-on-chip devices as intestinal inflammation models and their future for studying multifactorial diseases
Susanne Taavitsainen, Kati Juuti-Uusitalo, Kalle Kurppa, Katri Lindfors, Pasi Kallio and Minna Kellomäki
Front. Lab. Chip. Technol., 2024, 2
DOI: 10.3389/frlct.2023.1337945

Microfluidics for the study of mechanotransduction
Christian M Griffith, Stephanie A Huang, Crescentia Cho, Tanmay M Khare, Matthew Rich, Gi-hun Lee, Frances S Ligler, Brian O Diekman and William J Polacheck
J. Phys. D: Appl. Phys., 2020, 53, 224004
DOI: 10.1088/1361-6463/ab78d4

1H-NMR Profiling of Short-Chain Fatty Acid Content from a Physiologically Accurate Gut-on-a-Chip Device
Brandaise Martinez, Luke A. Schwerdtfeger, Alec Richardson, Stuart A. Tobet and Charles S. Henry
Anal. Chem., 2022, 94, 9987
DOI: 10.1021/acs.analchem.1c05146

Alternative Testing Strategies for Nanomaterials: State of the Science and Considerations for Risk Analysis
J. A. Shatkin and K. J. Ong
Risk Analysis, 2016, 36, 1564
DOI: 10.1111/risa.12642

Thinking in 3 dimensions: philosophies of the microenvironment in organoids and organs-on-chip
Silvia Caianiello, Marta Bertolaso and Guglielmo Militello
HPLS, 2023, 45
DOI: 10.1007/s40656-023-00560-z

Development of a human primary gut-on-a-chip to model inflammatory processes
Claudia Beaurivage, Auste Kanapeckaite, Cindy Loomans, Kai S. Erdmann, Jan Stallen and Richard A. J. Janssen
Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-78359-2

In vitro models replicating the human intestinal epithelium for absorption and metabolism studies: A systematic review
Arianna Fedi, Chiara Vitale, Giulia Ponschin, Seyoum Ayehunie, Marco Fato and Silvia Scaglione
Journal of Controlled Release, 2021, 335, 247
DOI: 10.1016/j.jconrel.2021.05.028

“Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration
Oksana A. Zhukova, Iuliia V. Ozerskaya, Dmitry V. Basmanov, Vsevolod Yu. Stolyarov, Vladimir G. Bogush, Vladimir V. Kolesov, Kirill A. Zykov, Gaukhar M. Yusubalieva and Vladimir P. Baklaushev
Journal of Clinical Practice, 2024, 15, 70
DOI: 10.17816/clinpract637140

Organs-on-chips: research and commercial perspectives
Aarathi Balijepalli and Vaibhav Sivaramakrishan
Drug Discovery Today, 2017, 22, 397
DOI: 10.1016/j.drudis.2016.11.009

Clinical translation of microbe‐based therapies: Current clinical landscape and preclinical outlook
Ava M. Vargason and Aaron C. Anselmo
Bioengineering & Transla Med, 2018, 3, 124
DOI: 10.1002/btm2.10093

In vitro enteroid-derived three-dimensional tissue model of human small intestinal epithelium with innate immune responses
Ying Chen, Wenda Zhou, Terrence Roh, Mary K. Estes, David L. Kaplan and Shree Ram Singh
PLoS ONE, 2017, 12, e0187880
DOI: 10.1371/journal.pone.0187880

Intestine-on-a-chip: Next level in vitro research model of the human intestine
Joanne M. Donkers, Hossein Eslami Amirabadi and Evita van de Steeg
Current Opinion in Toxicology, 2021, 25, 6
DOI: 10.1016/j.cotox.2020.11.002

Intestinalin vitrocell culture models and their potential to study the effect of food components on intestinal inflammation
Maria del Carmen Ponce de León-Rodríguez, Jean-Pierre Guyot and Caroline Laurent-Babot
Critical Reviews in Food Science and Nutrition, 2019, 59, 3648
DOI: 10.1080/10408398.2018.1506734

Development of Intestinal Scaffolds that Mimic Native Mammalian Intestinal Tissue
Mitchell R. Ladd, Cait M. Costello, Carolyn Gosztyla, Adam D. Werts, Blake Johnson, William B. Fulton, Laura Y. Martin, Elizabeth J. Redfield, Bryan Crawford, Rohan Panaparambil, Chhinder P. Sodhi, John C. March and David J. Hackam
Tissue Engineering Part A, 2019, 25, 1225
DOI: 10.1089/ten.tea.2018.0239

Extracellular Matrix Mechanical Properties and Regulation of the Intestinal Stem Cells: When Mechanics Control Fate
Lauriane Onfroy-Roy, Dimitri Hamel, Julie Foncy, Laurent Malaquin and Audrey Ferrand
Cells, 2020, 9, 2629
DOI: 10.3390/cells9122629

Effects of Porous Size and Membrane Pattern on Shear Stress Characteristic in Gut-on-a-Chip with Peristalsis Motion
Pannasit Borwornpiyawat, Ekachai Juntasaro, Sasitorn Aueviriyavit, Varangrat Juntasaro, Witsaroot Sripumkhai, Pattaraluck Pattamang, Rattanawan Meananeatra, Kornphimol Kulthong, Ratjika Wongwanakul, Numfon Khemthongcharoen, Nithi Atthi and Wutthinan Jeamsaksiri
Micromachines, 2022, 14, 22
DOI: 10.3390/mi14010022

Organ‐On‐A‐Chip Platforms: A Convergence of Advanced Materials, Cells, and Microscale Technologies
Samad Ahadian, Robert Civitarese, Dawn Bannerman, Mohammad Hossein Mohammadi, Rick Lu, Erika Wang, Locke Davenport‐Huyer, Ben Lai, Boyang Zhang, Yimu Zhao, Serena Mandla, Anastasia Korolj and Milica Radisic
Adv Healthcare Materials, 2018, 7
DOI: 10.1002/adhm.201700506

In vitro and ex vivo modeling of enteric bacterial infections
Nayere Taebnia, Ute Römling and Volker M. Lauschke
Gut Microbes, 2023, 15
DOI: 10.1080/19490976.2022.2158034

A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transepithelial Transport of Food Compounds
Arancha Hevia, Patricia Ruas-Madiedo, Miguel Angelo Faria, Valérie Petit, Bruna Alves, Paula Alvito, Elena Arranz, Shanna Bastiaan-Net, Milena Corredig, Wieneke Dijk, Didier Dupont, Linda Giblin, Brigitte Anna Graf, Alina Kondrashina, Helena Ramos, Lorena Ruiz, Marta Santos-Hernández, Laura Soriano-Romaní, Lidia Tomás-Cobos, Santiaga María Vivanco-Maroto, Isidra Recio and Beatriz Miralles
J. Agric. Food Chem., 2023, 71, 19265
DOI: 10.1021/acs.jafc.3c05479

Potential of Drug Efficacy Evaluation in Lung and Kidney Cancer Models Using Organ-on-a-Chip Technology
Seong-Hye Hwang, Sangchul Lee, Jee Yoon Park, Jessie Sungyun Jeon, Young-Jae Cho and Sejoong Kim
Micromachines, 2021, 12, 215
DOI: 10.3390/mi12020215

Organoids in gastrointestinal diseases: from experimental models to clinical translation
Claudia Günther, Beate Winner, Markus F Neurath and Thaddeus S Stappenbeck
Gut, 2022, 71, 1892
DOI: 10.1136/gutjnl-2021-326560

Electrochemical Gut-on-Chip systems as the next generation platforms for studying mucosal redox biology
Daniel Kaufman and Hadar Ben-Yoav
Current Opinion in Electrochemistry, 2024, 44, 101442
DOI: 10.1016/j.coelec.2024.101442

Multifunctional Bioreactor System for Human Intestine Tissues
Wenda Zhou, Ying Chen, Terrence Roh, Yinan Lin, Shengjie Ling, Siwei Zhao, James D. Lin, Noor Khalil, Dana M. Cairns, Eleana Manousiouthakis, Megan Tse and David L. Kaplan
ACS Biomater. Sci. Eng., 2018, 4, 231
DOI: 10.1021/acsbiomaterials.7b00794

A Comprehensive Review on Recent Advances and Considerations for the Selection of Cell-based In-vitro Techniques for the Assessment of Permeability of Drug Molecules
Rajat Garg and Anuj Garg
CDD, 2023, 20, 526
DOI: 10.2174/1567201819666220411115108

Single-cell transcriptomic mapping of intestinal epithelium that undergoes 3D morphogenesis and mechanodynamic stimulation in a gut-on-a-chip
Woojung Shin, Zhe Su, S. Stephen Yi and Hyun Jung Kim
iScience, 2022, 25, 105521
DOI: 10.1016/j.isci.2022.105521

Challenges and Opportunities in the Oral Delivery of Recombinant Biologics
Solene Masloh, Maxime Culot, Fabien Gosselet, Anne Chevrel, Leonardo Scapozza and Magali Zeisser Labouebe
Pharmaceutics, 2023, 15, 1415
DOI: 10.3390/pharmaceutics15051415

Comparison of 2D, 3D In Vitro, and Ex Vivo Platforms for Modeling the Rat Small Intestine
Shani Elias-Kirma, Reece McCoy, Douglas van Niekerk, Verena Stoeger, Sophie Oldroyd, Emma Sumner, Achilleas Savva and Róisín M. Owens
Bioengineering, 2026, 13, 349
DOI: 10.3390/bioengineering13030349

Gut-on-Chip microphysiological systems: Latest advances in the integration of sensing strategies and adoption of mature detection mechanisms
Maria Assunta Signore, Chiara De Pascali, Lucia Giampetruzzi, Pietro Aleardo Siciliano and Luca Francioso
Sensing and Bio-Sensing Research, 2021, 33, 100443
DOI: 10.1016/j.sbsr.2021.100443

Design of an Integrated Microvascularized Human Skin-on-a-Chip Tissue Equivalent Model
Christian F. E. Jones, Stefania Di Cio, John T. Connelly and Julien E. Gautrot
Front. Bioeng. Biotechnol., 2022, 10
DOI: 10.3389/fbioe.2022.915702

The Progress of Intestinal Epithelial Models from Cell Lines to Gut-On-Chip
Shafaque Rahman, Mohammed Ghiboub, Joanne M. Donkers, Evita van de Steeg, Eric A. F. van Tol, Theodorus B. M. Hakvoort and Wouter J. de Jonge
IJMS, 2021, 22, 13472
DOI: 10.3390/ijms222413472

Drug Disposition in the Lower Gastrointestinal Tract: Targeting and Monitoring
Glenn Lemmens, Arno Van Camp, Stephanie Kourula, Tim Vanuytsel and Patrick Augustijns
Pharmaceutics, 2021, 13, 161
DOI: 10.3390/pharmaceutics13020161

Nanoparticles for oral delivery: Design, evaluation and state-of-the-art
Abhijit A. Date, Justin Hanes and Laura M. Ensign
Journal of Controlled Release, 2016, 240, 504
DOI: 10.1016/j.jconrel.2016.06.016

Passive-Flow-Based MPS: Emerging Physiological Flow-Mimetic Platforms for Studying Effects of Flow on Single Tissues and Inter-tissue Interactions
Geonho Jin, Dohyung Kim, Seonghun Mun and Seokyoung Bang
BioChip J, 2024, 18, 186
DOI: 10.1007/s13206-024-00141-7

In Vitro Immune Organs-on-Chip for Drug Development: A Review
Aya Shanti, Jeremy Teo and Cesare Stefanini
Pharmaceutics, 2018, 10, 278
DOI: 10.3390/pharmaceutics10040278

Drug delivery to the inflamed intestinal mucosa – targeting technologies and human cell culture models for better therapies of IBD
Olga Hartwig, Maryam A. Shetab Boushehri, Karim S. Shalaby, Brigitta Loretz, Alf Lamprecht and Claus-Michael Lehr
Advanced Drug Delivery Reviews, 2021, 175, 113828
DOI: 10.1016/j.addr.2021.113828

Evaluation of Hybrid Vesicles in an Intestinal Cell Model Based on Structured Paper Chips
Paula De Dios Andres, Isabella N. Westensee, Edit Brodszkij, Miguel A. Ramos-Docampo, Noga Gal and Brigitte Städler
Biomacromolecules, 2021, 22, 3860
DOI: 10.1021/acs.biomac.1c00686

Mucus-producing 3D cell culture models
Maria Helena Macedo, Ana Baião, Soraia Pinto, Andreia S. Barros, Helena Almeida, Andreia Almeida, José das Neves and Bruno Sarmento
Advanced Drug Delivery Reviews, 2021, 178, 113993
DOI: 10.1016/j.addr.2021.113993

Robotic fluidic coupling and interrogation of multiple vascularized organ chips
Richard Novak, Miles Ingram, Susan Marquez, Debarun Das, Aaron Delahanty, Anna Herland, Ben M. Maoz, Sauveur S. F. Jeanty, Mahadevabharath R. Somayaji, Morgan Burt, Elizabeth Calamari, Angeliki Chalkiadaki, Alexander Cho, Youngjae Choe, David Benson Chou, Michael Cronce, Stephanie Dauth, Toni Divic, Jose Fernandez-Alcon, Thomas Ferrante, John Ferrier, Edward A. FitzGerald, Rachel Fleming, Sasan Jalili-Firoozinezhad, Thomas Grevesse, Josue A. Goss, Tiama Hamkins-Indik, Olivier Henry, Chris Hinojosa, Tessa Huffstater, Kyung-Jin Jang, Ville Kujala, Lian Leng, Robert Mannix, Yuka Milton, Janna Nawroth, Bret A. Nestor, Carlos F. Ng, Blakely O’Connor, Tae-Eun Park, Henry Sanchez, Josiah Sliz, Alexandra Sontheimer-Phelps, Ben Swenor, Guy Thompson, George J. Touloumes, Zachary Tranchemontagne, Norman Wen, Moran Yadid, Anthony Bahinski, Geraldine A. Hamilton, Daniel Levner, Oren Levy, Andrzej Przekwas, Rachelle Prantil-Baun, Kevin K. Parker and Donald E. Ingber
Nat Biomed Eng, 2020, 4, 407
DOI: 10.1038/s41551-019-0497-x

Development of Gut-Mucus Chip for Intestinal Absorption Study
Seung Yeon Lee, Yujeong Lee, Nakwon Choi, Hong Nam Kim, Bumsang Kim and Jong Hwan Sung
BioChip J, 2023, 17, 230
DOI: 10.1007/s13206-023-00097-0

Biomimetic villi-crypt scaffold-on-chip with tunable mechanical properties for intestinal epithelium modeling
Lorenzo Zavagna, Marta R. Casanova, Lara Pierantoni, Mariana R. Carvalho, Joana Silva-Correia, Teresa Macchi, Fabiola Troisi, Roberto Giovannoni, Rui L. Reis, Serena Danti and J. Miguel Oliveira
Materials Today Bio, 2026, 38, 103134
DOI: 10.1016/j.mtbio.2026.103134

Advancements in Microfluidic Organ-on-a-chip for Oral Medicine
Sizheng Fan, Yongcheng Ge, Bingdao Li, Pengyuan Liu and Xiaoqiu Liu
International Dental Journal, 2025, 75, 100925
DOI: 10.1016/j.identj.2025.100925

Hydrophobic Patterning‐Based 3D Microfluidic Cell Culture Assay
Sewoon Han, Junghyun Kim, Rui Li, Alice Ma, Vincent Kwan, Kevin Luong and Lydia L. Sohn
Adv Healthcare Materials, 2018, 7
DOI: 10.1002/adhm.201800122

Modeling the Glomerular Filtration Barrier and Intercellular Crosstalk
Kerstin Ebefors, Emelie Lassén, Nanditha Anandakrishnan, Evren U. Azeloglu and Ilse S. Daehn
Front. Physiol., 2021, 12
DOI: 10.3389/fphys.2021.689083

Formation of Human Colonic Crypt Array by Application of Chemical Gradients Across a Shaped Epithelial Monolayer
Yuli Wang, Raehyun Kim, Dulan B. Gunasekara, Mark I. Reed, Matthew DiSalvo, Daniel L. Nguyen, Scott J. Bultman, Christopher E. Sims, Scott T. Magness and Nancy L. Allbritton
Cellular and Molecular Gastroenterology and Hepatology, 2018, 5, 113
DOI: 10.1016/j.jcmgh.2017.10.007

Enhanced Utilization of Induced Pluripotent Stem Cell–Derived Human Intestinal Organoids Using Microengineered Chips
Michael J. Workman, John P. Gleeson, Elissa J. Troisi, Hannah Q. Estrada, S. Jordan Kerns, Christopher D. Hinojosa, Geraldine A. Hamilton, Stephan R. Targan, Clive N. Svendsen and Robert J. Barrett
Cellular and Molecular Gastroenterology and Hepatology, 2018, 5, 669
DOI: 10.1016/j.jcmgh.2017.12.008

Sensor-free and Sensor-based Heart-on-a-chip Platform: A Review of Design and Applications
Hao Wan, Chenlei Gu, Ying Gan, Xinwei Wei, Kai Zhu, Ning Hu and Ping Wang
CPD, 2019, 24, 5375
DOI: 10.2174/1381612825666190207170004

Modeling Barrier Tissues In Vitro: Methods, Achievements, and Challenges
Courtney M. Sakolish, Mandy B. Esch, James J. Hickman, Michael L. Shuler and Gretchen J. Mahler
EBioMedicine, 2016, 5, 30
DOI: 10.1016/j.ebiom.2016.02.023

Recent advances in microfluidic 3D cellular scaffolds for drug assays
Jing Wu, Qiushui Chen, Wu Liu, Ziyi He and Jin-Ming Lin
TrAC Trends in Analytical Chemistry, 2017, 87, 19
DOI: 10.1016/j.trac.2016.11.009

Coculture of primary human colon monolayer with human gut bacteria
Jianbo Zhang, Victor Hernandez-Gordillo, Martin Trapecar, Charles Wright, Mao Taketani, Kirsten Schneider, Wen Li Kelly Chen, Eric Stas, David T. Breault, Rebecca L. Carrier, Christopher A. Voigt and Linda G. Griffith
Nat Protoc, 2021, 16, 3874
DOI: 10.1038/s41596-021-00562-w

Pharm-lab-on-a-chip: advancements in pharmaceutical and pharmacological analysis through microfluidic chip technology: a comprehensive review
Raha F. Shukr, Hemn A. Qader, Idrees B. Qader, Sameera Sh. Mohammed Ameen and Khalid M. Omer
Chem. Pap., 2025, 79, 7247
DOI: 10.1007/s11696-025-04301-3

Intestinal organoids: A new paradigm for engineering intestinal epithelium in vitro
Sara Rahmani, Natalia M. Breyner, Hsuan-Ming Su, Elena F. Verdu and Tohid F. Didar
Biomaterials, 2019, 194, 195
DOI: 10.1016/j.biomaterials.2018.12.006

Organotypic and Microphysiological Models of Liver, Gut, and Kidney for Studies of Drug Metabolism, Pharmacokinetics, and Toxicity
Joanne X. Shen, Sonia Youhanna, Reza Zandi Shafagh, Julianna Kele and Volker M. Lauschke
Chem. Res. Toxicol., 2020, 33, 38
DOI: 10.1021/acs.chemrestox.9b00245

Regional Expression Levels of Drug Transporters and Metabolizing Enzymes along the Pig and Human Intestinal Tract and Comparison with Caco-2 Cells
Stefan F.C. Vaessen, Marola M.H. van Lipzig, Raymond H.H. Pieters, Cyrille A.M. Krul, Heleen M. Wortelboer and Evita van de Steeg
Drug Metabolism and Disposition, 2017, 45, 353
DOI: 10.1124/dmd.116.072231

Organ-on-a-chip: Quo vademus? Applications and regulatory status
Maria Mendes, Ana Sofia Morais, Ana Carlos, João José Sousa, Alberto Canelas Pais, Silvia M. Mihăilă and Carla Vitorino
Colloids and Surfaces B: Biointerfaces, 2025, 249, 114507
DOI: 10.1016/j.colsurfb.2025.114507

Mechanical Stimuli Affect Escherichia coli Heat-Stable Enterotoxin-Cyclic GMP Signaling in a Human Enteroid Intestine-Chip Model
Laxmi Sunuwar, Jianyi Yin, Magdalena Kasendra, Katia Karalis, James Kaper, James Fleckenstein, Mark Donowitz and Manuela Raffatellu
Infect Immun, 2020, 88
DOI: 10.1128/IAI.00866-19

Mimicking Human Pathophysiology in Organ‐on‐Chip Devices
Ozlem Yesil‐Celiktas, Shabir Hassan, Amir K. Miri, Sushila Maharjan, Rawan Al‐kharboosh, Alfredo Quiñones‐Hinojosa and Yu Shrike Zhang
Adv. Biosys., 2018, 2
DOI: 10.1002/adbi.201800109

Materials and Microenvironments for Engineering the Intestinal Epithelium
Jessica Snyder, Chia-Ming Wang, An Qi Zhang, Yuan Li, Joshua Luchan, Sanjin Hosic, Ryan Koppes, Rebecca Lyn Carrier and Abigail Koppes
Ann Biomed Eng, 2020, 48, 1916
DOI: 10.1007/s10439-020-02470-8

Ultrathin Alumina Membranes as Scaffold for Epithelial Cell Culture from the Intestine of Rainbow Trout
Carolin Drieschner, Matteo Minghetti, Songmei Wu, Philippe Renaud and Kristin Schirmer
ACS Appl. Mater. Interfaces, 2017, 9, 9496
DOI: 10.1021/acsami.7b00705

Mechanical forces directing intestinal form and function
Ronja M. Houtekamer, Mirjam C. van der Net, Madelon M. Maurice and Martijn Gloerich
Current Biology, 2022, 32, R791
DOI: 10.1016/j.cub.2022.05.041

New approaches to increase intestinal length: Methods used for intestinal regeneration and bioengineering
Ali Shirafkan, Mauro Montalbano, Joshua McGuire, Cristiana Rastellini and Luca Cicalese
WJT, 2016, 6, 1
DOI: 10.5500/wjt.v6.i1.1

Human stroma and epithelium co-culture in a microfluidic model of a human prostate gland
L. Jiang, F. Ivich, S. Tahsin, M. Tran, S. B. Frank, C. K. Miranti and Y. Zohar
Biomicrofluidics, 2019, 13
DOI: 10.1063/1.5126714

Intestinal organoids in coculture: redefining the boundaries of gut mucosa ex vivo modeling
Viktoria Hentschel, Thomas Seufferlein and Milena Armacki
American Journal of Physiology-Gastrointestinal and Liver Physiology, 2021, 321, G693
DOI: 10.1152/ajpgi.00043.2021

Dynamic flow and shear stress as key parameters for intestinal cells morphology and polarization in an organ-on-a-chip model
Chiara A. M. Fois, Aaron Schindeler, Peter Valtchev and Fariba Dehghani
Biomed Microdevices, 2021, 23
DOI: 10.1007/s10544-021-00591-y

Advancing Regulatory Science Through Innovation: In Vitro Microphysiological Systems
Suzanne Fitzpatrick and Robert Sprando
Cellular and Molecular Gastroenterology and Hepatology, 2019, 7, 239
DOI: 10.1016/j.jcmgh.2018.08.004

‘You-on-a-chip’ for precision medicine
Lucie A. Low and Danilo A. Tagle
Expert Review of Precision Medicine and Drug Development, 2018, 3, 137
DOI: 10.1080/23808993.2018.1456333

Structure-activity relationships of bioactive polysaccharides extracted from macroalgae towards biomedical application: A review
Yinyi Fu, Haixin Jiao, Jianzhong Sun, Charles Obinwanne Okoye, Hongxing Zhang, Yan Li, Xuechu Lu, Qianqian Wang and Jun Liu
Carbohydrate Polymers, 2024, 324, 121533
DOI: 10.1016/j.carbpol.2023.121533

Navigating Through Cell-Based In vitro Models Available for Prediction of Intestinal Permeability and Metabolism: Are We Ready for 3D?
Yesenia L. Franco, Lais Da Silva and Rodrigo Cristofoletti
AAPS J, 2021, 24
DOI: 10.1208/s12248-021-00665-y

Advances of microfluidic intestine-on-a-chip for analyzing anti-inflammation of food
Duo Liang, Wentao Su and Mingqian Tan
Critical Reviews in Food Science and Nutrition, 2022, 62, 4418
DOI: 10.1080/10408398.2021.1875395

Gut-on-a-Chip Models: Current and Future Perspectives for Host–Microbial Interactions Research
Moran Morelli, Dorota Kurek, Chee Ping Ng and Karla Queiroz
Biomedicines, 2023, 11, 619
DOI: 10.3390/biomedicines11020619

The translational roadmap of the gut models, focusing on gut-on-chip
Giulia Malaguarnera, Miriam Graute and Antoni Homs Corbera
Open Res Europe, 2021, 1, 62
DOI: 10.12688/openreseurope.13709.1

Stem cell-based retina models
Kevin Achberger, Jasmin C. Haderspeck, Alexander Kleger and Stefan Liebau
Advanced Drug Delivery Reviews, 2019, 140, 33
DOI: 10.1016/j.addr.2018.05.005

A Role for 3D Printing in Kidney-on-a-Chip Platforms
Ryan D. Sochol, Navin R. Gupta and Joseph V. Bonventre
Curr Transpl Rep, 2016, 3, 82
DOI: 10.1007/s40472-016-0085-x

Pharma’s Bio-AI revolution
Isaac Bentwich
Drug Discovery Today, 2023, 28, 103515
DOI: 10.1016/j.drudis.2023.103515

“Good Fences Make Good Neighbors”: How does the Human Gut Microchip Unravel Mechanism of Intestinal Inflammation?
Woojung Shin, Landon A. Hackley and Hyun Jung Kim
Gut Microbes, 2020, 11, 581
DOI: 10.1080/19490976.2019.1626684

Engineering Microfluidic Organoid-on-a-Chip Platforms
Fang Yu, Walter Hunziker and Deepak Choudhury
Micromachines, 2019, 10, 165
DOI: 10.3390/mi10030165

Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation
Sandra Pérez‐Rodríguez, José Manuel García‐Aznar and Jesús Gonzalo‐Asensio
Microbial Biotechnology, 2022, 15, 395
DOI: 10.1111/1751-7915.13775

Intestine-on-a-Chip Microfluidic Model for Efficient in Vitro Screening of Oral Chemotherapeutic Uptake
Kyall Pocock, Ludivine Delon, Vaskor Bala, Shasha Rao, Craig Priest, Clive Prestidge and Benjamin Thierry
ACS Biomater. Sci. Eng., 2017, 3, 951
DOI: 10.1021/acsbiomaterials.7b00023

Recent advances in biofabricated gut models to understand the gut-brain axis in neurological diseases
Hohyeon Han and Jinah Jang
Front. Med. Technol., 2022, 4
DOI: 10.3389/fmedt.2022.931411

Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport
Karen E. Samy, Elizabeth S. Levy, Kiet Phong, Benjamin Demaree, Adam R. Abate and Tejal A. Desai
Sci Rep, 2019, 9
DOI: 10.1038/s41598-019-46408-0

Models of the Gut for Analyzing the Impact of Food and Drugs
Chiara Anna Maria Fois, Thi Yen Loan Le, Aaron Schindeler, Sina Naficy, Dale David McClure, Mark Norman Read, Peter Valtchev, Ali Khademhosseini and Fariba Dehghani
Adv Healthcare Materials, 2019, 8
DOI: 10.1002/adhm.201900968

Dynamic single cell analysis in a proximal-tubule-on-chip reveals heterogeneous epithelial colonization strategies of uropathogenic Escherichia coli under shear stress
Haris Antypas, Tianqi Zhang, Ferdinand X Choong, Keira Melican and Agneta Richter-Dahlfors
FEMS Microbes, 2023, 4
DOI: 10.1093/femsmc/xtad007

Intestinal microecology dysbiosis in inflammatory bowel disease: Pathogenesis and therapeutic strategies
Yue Yin, Weibo Feng, Xilang Chen, Jie Chen, Guodong Wang, Limin Xia and Kaichun Wu
TIME, 2024, 2, 100092
DOI: 10.59717/j.xinn-med.2024.100092

Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease
Jiandong Yang, Yoshikazu Hirai, Kei Iida, Shinji Ito, Marika Trumm, Shiho Terada, Risako Sakai, Toshiyuki Tsuchiya, Osamu Tabata and Ken-ichiro Kamei
Commun Biol, 2023, 6
DOI: 10.1038/s42003-023-04710-8

Intestinal Stem Cell-on-Chip to Study Human Host-Microbiota Interaction
Fatina Siwczak, Elise Loffet, Mathilda Kaminska, Hristina Koceva, Maxime M. Mahe and Alexander S. Mosig
Front. Immunol., 2021, 12
DOI: 10.3389/fimmu.2021.798552

The in vitro intestinal cell model: different co-cultured cells create different applications
Xingyu Zou, Yue Liu, Mengyao Cui, Qing Wan and Xiaoqin Chu
Journal of Drug Targeting, 2024, 32, 529
DOI: 10.1080/1061186X.2024.2333877

Fabrication of 3D scaffolds reproducing intestinal epithelium topography by high-resolution 3D stereolithography
Justine Creff, Rémi Courson, Thomas Mangeat, Julie Foncy, Sandrine Souleille, C. Thibault, Arnaud Besson and Laurent Malaquin
Biomaterials, 2019, 221, 119404
DOI: 10.1016/j.biomaterials.2019.119404

PDMS-based porous membrane for medical applications: design, development, and fabrication
Mohsen Mashhadi Keshtiban, Mahdi Moghimi Zand, Amirali Ebadi and Zahra Azizi
Biomed. Mater., 2023, 18, 045012
DOI: 10.1088/1748-605X/acbddb

Development of a dual-flow tissue perfusion device for modeling the gastrointestinal tract–brain axis
Lydia Baldwin, Emily J. Jones, Alexander Iles, Simon R. Carding, Nicole Pamme, Charlotte E. Dyer and John Greenman
Biomicrofluidics, 2023, 17
DOI: 10.1063/5.0168953

Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems
Benjamin O. Murray, Carlos Flores, Corin Williams, Deborah A. Flusberg, Elizabeth E. Marr, Karolina M. Kwiatkowska, Joseph L. Charest, Brett C. Isenberg and Jennifer L. Rohn
Front. Cell. Infect. Microbiol., 2021, 11
DOI: 10.3389/fcimb.2021.691210

Thin microporous polydimethylsiloxane membrane prepared by phase separation and its applications for cell culture
Jin Hong Yap, Hong Zhang, Yosuke Okamura and Hiroshi Kimura
Materialia, 2024, 38, 102247
DOI: 10.1016/j.mtla.2024.102247

The impact of dietary fibres on the physiological processes governing small intestinal digestive processes
Peter I. Chater, Matthew D. Wilcox, Jeffrey P. Pearson and Iain A. Brownlee
Bioactive Carbohydrates and Dietary Fibre, 2015, 6, 117
DOI: 10.1016/j.bcdf.2015.09.002

Compartmentalized Culture of Perivascular Stroma and Endothelial Cells in a Microfluidic Model of the Human Endometrium
Juan S. Gnecco, Virginia Pensabene, David J. Li, Tianbing Ding, Elliot E. Hui, Kaylon L. Bruner-Tran and Kevin G. Osteen
Ann Biomed Eng, 2017, 45, 1758
DOI: 10.1007/s10439-017-1797-5

Microfluidic Organ-on-a-Chip System for Disease Modeling and Drug Development
Zening Li, Jianan Hui, Panhui Yang and Hongju Mao
Biosensors, 2022, 12, 370
DOI: 10.3390/bios12060370

Human gut-on-a-chip technology: will this revolutionize our understanding of IBD and future treatments?
Jaewon Lee, Jin-Ha Choi and Hyun Jung Kim
Expert Review of Gastroenterology & Hepatology, 2016, 1
DOI: 10.1080/17474124.2016.1200466

Three-dimensional in vitro gut model on a villi-shaped collagen scaffold
Banya Yi, Kyu Young Shim, Sang Keun Ha, Jeonghun Han, Hong-Hoa Hoang, Inwook Choi, Sungsu Park and Jong Hwan Sung
BioChip J, 2017, 11, 219
DOI: 10.1007/s13206-017-1307-8

The motility of Entamoeba histolytica: finding ways to understand intestinal amoebiasis
Arturo Aguilar-Rojas, Jean-Christophe Olivo-Marin and Nancy Guillen
Current Opinion in Microbiology, 2016, 34, 24
DOI: 10.1016/j.mib.2016.07.016

A New 3D Colon on a Chip to Decipher the Influence of Mechanical Forces on the Physiological Cellular Ecosystem
Moencopi Bernheim‐Dennery, Lauriane Gérémie, Julie Brun, Lucas Chassatte, Giacomo Gropplero, Réda Bouras, Jieun Choo, Bertrand Cinquin, Alba Marcellan, Danijela Matic Vignjevic and Stéphanie Descroix
Adv Healthcare Materials, 2026
DOI: 10.1002/adhm.202505483

An Advanced Human Intestinal Coculture Model Reveals Compartmentalized Host and Pathogen Strategies during Salmonella Infection
Leon N. Schulte, Matthias Schweinlin, Alexander J. Westermann, Harshavardhan Janga, Sara C. Santos, Silke Appenzeller, Heike Walles, Jörg Vogel, Marco Metzger and Julian Parkhill
mBio, 2020, 11
DOI: 10.1128/mBio.03348-19

A three-dimensional immunocompetent intestine-on-chip model as in vitro platform for functional and microbial interaction studies
Michelle Maurer, Mark S. Gresnigt, Antonia Last, Tony Wollny, Florian Berlinghof, Rebecca Pospich, Zoltan Cseresnyes, Anna Medyukhina, Katja Graf, Marko Gröger, Martin Raasch, Fatina Siwczak, Sandor Nietzsche, Ilse D. Jacobsen, Marc Thilo Figge, Bernhard Hube, Otmar Huber and Alexander S. Mosig
Biomaterials, 2019, 220, 119396
DOI: 10.1016/j.biomaterials.2019.119396

Effects of Flavanols on Enteroendocrine Secretion
Carme Grau-Bové, Carlos González-Quilen, Ximena Terra, M. Teresa Blay, Raul Beltrán-Debón, Rosa Jorba-Martín, Beatriz Espina, Montserrat Pinent and Anna Ardévol
Biomolecules, 2020, 10, 844
DOI: 10.3390/biom10060844

Emerging Technologies for Gut Microbiome Research
Jason W. Arnold, Jeffrey Roach and M. Andrea Azcarate-Peril
Trends in Microbiology, 2016, 24, 887
DOI: 10.1016/j.tim.2016.06.008

Gut bioengineered models to study host-microbiota-probiotics interactions
Elise Delannoy, Alexandre Grassart and Catherine Daniel
Microbiome Res Rep., 2025, 4
DOI: 10.20517/mrr.2025.45

Intestinal Villi Model with Blood Capillaries Fabricated Using Collagen-Based Bioink and Dual-Cell-Printing Process
WonJin Kim and GeunHyung Kim
ACS Appl. Mater. Interfaces, 2018, 10, 41185
DOI: 10.1021/acsami.8b17410

Organs-on-a-chip
David Bovard, Anita Iskandar, Karsta Luettich, Julia Hoeng and Manuel C Peitsch
Toxicology Research and Application, 2017, 1
DOI: 10.1177/2397847317726351

Digesting the mechanobiology of the intestinal epithelium
Carlos Pérez-González, Gerardo Ceada, Marija Matejčić and Xavier Trepat
Current Opinion in Genetics & Development, 2022, 72, 82
DOI: 10.1016/j.gde.2021.10.005

A microfluidic chip based model for the study of full thickness human intestinal tissue using dual flow
A. Dawson, C. Dyer, J. Macfie, J. Davies, L. Karsai, J. Greenman and M. Jacobsen
Biomicrofluidics, 2016, 10
DOI: 10.1063/1.4964813

Turn-On Fluorescence Sensing of Oxygen with Dendrimer-Encapsulated Platinum Nanoparticles as Tunable Oxidase Mimics for Spatially Resolved Measurement of Oxygen Gradient in a Human Gut-on-a-Chip
Hyein Lee, Woojung Shin, Hyun Jung Kim and Joohoon Kim
Anal. Chem., 2021, 93, 16123
DOI: 10.1021/acs.analchem.1c03891

Cellular membrane protein MipA from E.coli Nissle 1917 protects against Salmonella infection
Yunqing Xiang, Huipai Peng, Yanxia Li, Hui Wen, Nan Li, Xiaozhi Liu, Guodong Yuan, Yong Shi and Shuqiang Huang
The ISME Journal, 2026, 20
DOI: 10.1093/ismejo/wrag054

Micro-patterned endogenous stroma equivalent induces polarized crypt-villus architecture of human small intestinal epithelium
Vincenza De Gregorio, Giorgia Imparato, Francesco Urciuolo and Paolo A. Netti
Acta Biomaterialia, 2018, 81, 43
DOI: 10.1016/j.actbio.2018.09.061

In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels
Samuel S. Hinman, Yuli Wang, Raehyun Kim and Nancy L. Allbritton
Nat Protoc, 2021, 16, 352
DOI: 10.1038/s41596-020-00419-8

Leveraging Organ‐on‐Chip Models to Investigate Host–Microbiota Dynamics and Targeted Therapies for Inflammatory Bowel Disease
Tim Kaden, Raquel Alonso‐Román, Johannes Stallhofer, Mark S. Gresnigt, Bernhard Hube and Alexander S. Mosig
Adv Healthcare Materials, 2025, 14
DOI: 10.1002/adhm.202402756

Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip
Hyun Jung Kim, Hu Li, James J. Collins and Donald E. Ingber
Proc. Natl. Acad. Sci. U.S.A., 2016, 113
DOI: 10.1073/pnas.1522193112

Spatial structure, chemotaxis and quorum sensing shape bacterial biomass accumulation in complex porous media
David Scheidweiler, Ankur Deep Bordoloi, Wenqiao Jiao, Vladimir Sentchilo, Monica Bollani, Audam Chhun, Philipp Engel and Pietro de Anna
Nat Commun, 2024, 15
DOI: 10.1038/s41467-023-44267-y

Advances in understanding of the innate immune response to human norovirus infection using organoid models
Wadzanai P. Mboko, Preeti Chhabra, Marta Diez Valcarce, Veronica Costantini and Jan Vinjé
Journal of General Virology, 2022, 103
DOI: 10.1099/jgv.0.001720

Advanced gut-on-chips for assessing carotenoid absorption, metabolism, and transport
Hui Wang, Cong Xu, Mingqian Tan and Wentao Su
Critical Reviews in Food Science and Nutrition, 2025, 65, 1344
DOI: 10.1080/10408398.2023.2293250

Advances in dynamic microphysiological organ-on-a-chip: Design principle and its biomedical application
Sang Hun Lee and Bong-Hyun Jun
Journal of Industrial and Engineering Chemistry, 2019, 71, 65
DOI: 10.1016/j.jiec.2018.11.041

ChipNMR: Hyperpolarized NMR for Noninvasive Metabolic Flux Analysis in Perfused Microfluidic Chips
Thomas B. Wareham Mathiassen, Juan D. Sánchez-Heredia, Ke-Chuan Wang, Cajsa R. Haupt, Magnus Karlsson, Alexander Jönsson, Martin Dufva, Roland Thuenauer and Pernille Rose Jensen
Anal. Chem., 2026, 98, 1891
DOI: 10.1021/acs.analchem.5c04058

Dual-Material 3D-Printed Intestinal Model Devices with Integrated Villi-like Scaffolds
Nayere Taebnia, Rujing Zhang, Emil B. Kromann, Alireza Dolatshahi-Pirouz, Thomas L. Andresen and Niels B. Larsen
ACS Appl. Mater. Interfaces, 2021, 13, 58434
DOI: 10.1021/acsami.1c22185

Organs-on-Chips Platforms Are Everywhere: A Zoom on Biomedical Investigation
Mohamed Zommiti, Nathalie Connil, Ali Tahrioui, Anne Groboillot, Corinne Barbey, Yoan Konto-Ghiorghi, Olivier Lesouhaitier, Sylvie Chevalier and Marc G. J. Feuilloley
Bioengineering, 2022, 9, 646
DOI: 10.3390/bioengineering9110646

Evaluation of PepT1 transport of food-derived antihypertensive peptides, Ile-Pro-Pro and Leu-Lys-Pro using in vitro, ex vivo and in vivo transport models
John P. Gleeson, David J. Brayden and Sinéad M. Ryan
European Journal of Pharmaceutics and Biopharmaceutics, 2017, 115, 276
DOI: 10.1016/j.ejpb.2017.03.007

Capturing and Quantifying Particle Transcytosis with Microphysiological Intestine‐on‐Chip Models
Ludivine C. Delon, Matthew Faria, Zhengyang Jia, Stuart Johnston, Rachel Gibson, Clive A. Prestidge and Benjamin Thierry
Small Methods, 2023, 7
DOI: 10.1002/smtd.202200989

Primary exploration of host–microorganism interaction and enteritis treatment with an embedded membrane microfluidic chip of the human intestinal–vascular microsystem
Wei Zhao, Yuhan Yao, Tong Zhang, Huijun Lu, Xinlian Zhang, Linlin Zhao, Xi Chen, Jinhui Zhu, Guodong Sui and Wang Zhao
Front. Bioeng. Biotechnol., 2022, 10
DOI: 10.3389/fbioe.2022.1035647

Recent advances in intestinal fibrosis
Marco Vincenzo Lenti, Giovanni Santacroce, Giacomo Broglio, Carlo Maria Rossi and Antonio Di Sabatino
Molecular Aspects of Medicine, 2024, 96, 101251
DOI: 10.1016/j.mam.2024.101251

Robust bioengineered 3D functional human intestinal epithelium
Ying Chen, Yinan Lin, Kimberly M. Davis, Qianrui Wang, Jelena Rnjak-Kovacina, Chunmei Li, Ralph R. Isberg, Carol A. Kumamoto, Joan Mecsas and David L. Kaplan
Sci Rep, 2015, 5
DOI: 10.1038/srep13708

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology
Jeremy D. Caplin, Norma G. Granados, Myra R. James, Reza Montazami and Nastaran Hashemi
Adv Healthcare Materials, 2015, 4, 1426
DOI: 10.1002/adhm.201500040

Ultrathin Polymer Membranes with Patterned, Micrometric Pores for Organs-on-Chips
Virginia Pensabene, Lino Costa, Alexander Y. Terekhov, Juan S. Gnecco, John P. Wikswo and William H. Hofmeister
ACS Appl. Mater. Interfaces, 2016, 8, 22629
DOI: 10.1021/acsami.6b05754

Tumor Microenvironment Alters Chemoresistance of Hepatocellular Carcinoma Through CYP3A4 Metabolic Activity
Alican Özkan, Danielle L. Stolley, Erik N. K. Cressman, Matthew McMillin, Sharon DeMorrow, Thomas E. Yankeelov and Marissa Nichole Rylander
Front. Oncol., 2021, 11
DOI: 10.3389/fonc.2021.662135

Intestinal Models for Personalized Medicine: from Conventional Models to Microfluidic Primary Intestine-on-a-chip
Xiang-Guang Li, Ming-xia Chen, Su-qing Zhao and Xiu-qi Wang
Stem Cell Rev and Rep, 2022, 18, 2137
DOI: 10.1007/s12015-021-10205-y

Advances and Challenges in Recapitulating Human Pulmonary Systems: At the Cusp of Biology and Materials
Piyush Bajaj, Jennifer F. Harris, Jen-Huang Huang, Pulak Nath and Rashi Iyer
ACS Biomater. Sci. Eng., 2016, 2, 473
DOI: 10.1021/acsbiomaterials.5b00480

Hydrogel‐based three‐dimensional cell culture for organ‐on‐a‐chip applications
Seung Hwan Lee, Kyu Young Shim, Bumsang Kim and Jong Hwan Sung
Biotechnology Progress, 2017, 33, 580
DOI: 10.1002/btpr.2457

Advanced Microengineered Lung Models for Translational Drug Discovery
Brian F. Niemeyer, Peng Zhao, Rubin M. Tuder and Kambez H. Benam
SLAS Discovery, 2018, 23, 777
DOI: 10.1177/2472555218760217

Development of Porous and Flexible PTMC Membranes for In Vitro Organ Models Fabricated by Evaporation-Induced Phase Separation
Thijs Pasman, Danielle Baptista, Sander van Riet, Roman K. Truckenmüller, Pieter S. Hiemstra, Robbert J. Rottier, Dimitrios Stamatialis and André A. Poot
Membranes, 2020, 10, 330
DOI: 10.3390/membranes10110330

Organ-On-A-Chip (OOAC) Technology: Impact on Drug Screening and Personalized Medicine
Subhashinee Singh, Nabanita Das, Manisha Rathore, Ambresh Nishad, Nisha Kumari and Rajdeep Guha
Regen. Eng. Transl. Med., 2025
DOI: 10.1007/s40883-025-00409-w

Bioengineered Human Organ-on-Chip Reveals Intestinal Microenvironment and Mechanical Forces Impacting Shigella Infection
Alexandre Grassart, Valérie Malardé, Samy Gobaa, Anna Sartori-Rupp, Jordan Kerns, Katia Karalis, Benoit Marteyn, Philippe Sansonetti and Nathalie Sauvonnet
Cell Host & Microbe, 2019, 26, 435
DOI: 10.1016/j.chom.2019.08.007

Characterization of rat or human hepatocytes cultured in microphysiological systems (MPS) to identify hepatotoxicity
Shih-Yu Chang, Jenna L. Voellinger, Kirk P. Van Ness, Brian Chapron, Rachel M. Shaffer, Thomas Neumann, Collin C. White, Terrance J. Kavanagh, Edward J. Kelly and David L. Eaton
Toxicology in Vitro, 2017, 40, 170
DOI: 10.1016/j.tiv.2017.01.007

Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium
R.H. Dosh, A. Essa, N. Jordan-Mahy, C. Sammon and C.L. Le Maitre
Acta Biomaterialia, 2017, 62, 128
DOI: 10.1016/j.actbio.2017.08.035

Polymeric and biological membranes for organ-on-a-chip devices
Kendra Corral-Nájera, Gaurav Chauhan, Sergio O. Serna-Saldívar, Sergio O. Martínez-Chapa and Mohammad Mahdi Aeinehvand
Microsyst Nanoeng, 2023, 9
DOI: 10.1038/s41378-023-00579-z

Three dimensional human small intestine models for ADME-Tox studies
Jiajie Yu, Rebecca L. Carrier, John C. March and Linda G. Griffith
Drug Discovery Today, 2014, 19, 1587
DOI: 10.1016/j.drudis.2014.05.003

Strategies to control cellular spatial organization in microphysiological systems
Hung Dong Truong, Zhixing Ge, Elgene Chng, Y-Van Tran, Yusheng Zhang and Chwee Teck Lim
Microsyst Nanoeng, 2026, 12
DOI: 10.1038/s41378-025-01141-9

Emergence of infectious diseases and role of advanced nanomaterials in point-of-care diagnostics: a review
Kalaimani Markandan, Yong Wei Tiong, Revathy Sankaran, Sakthinathan Subramanian, Uma Devi Markandan, Vishal Chaudhary, Arshid Numan, Mohammad Khalid and Rashmi Walvekar
Biotechnology and Genetic Engineering Reviews, 2024, 40, 3438
DOI: 10.1080/02648725.2022.2127070

Advances and challenges in organ-on-chip technology: toward mimicking human physiology and disease in vitro
Dhiraj Kumar, Rahul Nadda and Ramjee Repaka
Med Biol Eng Comput, 2024, 62, 1925
DOI: 10.1007/s11517-024-03062-7

In vitro models of intestinal epithelium: Toward bioengineered systems
Justine Creff, Laurent Malaquin and Arnaud Besson
J Tissue Eng, 2021, 12
DOI: 10.1177/2041731420985202

Morphological and molecular characteristics of spheroid formation in HT-29 and Caco-2 colorectal cancer cell lines
Elmira Gheytanchi, Marzieh Naseri, Feridoun Karimi-Busheri, Fatemeh Atyabi, Ensie Sadat Mirsharif, Mahmood Bozorgmehr, Roya Ghods and Zahra Madjd
Cancer Cell Int, 2021, 21
DOI: 10.1186/s12935-021-01898-9

The emergence of 3D bioprinting in organ-on-chip systems
Kirsten Fetah, Peyton Tebon, Marcus J Goudie, James Eichenbaum, Li Ren, Natan Barros, Rohollah Nasiri, Samad Ahadian, Nureddin Ashammakhi, Mehmet R Dokmeci and Ali Khademhosseini
Prog. Biomed. Eng., 2019, 1, 012001
DOI: 10.1088/2516-1091/ab23df

Lung-on-a-chip: the future of respiratory disease models and pharmacological studies
Jesus Shrestha, Sajad Razavi Bazaz, Hamidreza Aboulkheyr Es, Dania Yaghobian Azari, Benjamin Thierry, Majid Ebrahimi Warkiani and Maliheh Ghadiri
Critical Reviews in Biotechnology, 2020, 40, 213
DOI: 10.1080/07388551.2019.1710458

Solid lipid nanoparticles for enhanced oral absorption: A review
Eman Salah, Mahmoud M. Abouelfetouh, Yuanhu Pan, Dongmei Chen and Shuyu Xie
Colloids and Surfaces B: Biointerfaces, 2020, 196, 111305
DOI: 10.1016/j.colsurfb.2020.111305

Recent Advances in the Gastrointestinal Complex in Vitro Model for ADME Studies
Kazuyoshi Michiba, Kengo Watanabe, Tomoki Imaoka and Daisuke Nakai
Pharmaceutics, 2023, 16, 37
DOI: 10.3390/pharmaceutics16010037

Establishment and evaluation of on-chip intestinal barrier biosystems based on microfluidic techniques
Hui Wang, Xiangyang Li, Pengcheng Shi, Xiaoyan You and Guoping Zhao
Materials Today Bio, 2024, 26, 101079
DOI: 10.1016/j.mtbio.2024.101079

Advances in Engineering Human Tissue Models
Chrysanthi-Maria Moysidou, Chiara Barberio and Róisín Meabh Owens
Front. Bioeng. Biotechnol., 2021, 8
DOI: 10.3389/fbioe.2020.620962

MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing
Patrick Mai, Jörg Hampl, Martin Baca, Dana Brauer, Sukhdeep Singh, Frank Weise, Justyna Borowiec, André Schmidt, Johanna Merle Küstner, Maren Klett, Michael Gebinoga, Insa S. Schroeder, Udo R. Markert, Felix Glahn, Berit Schumann, Diana Eckstein and Andreas Schober
Bioengineering, 2022, 9, 220
DOI: 10.3390/bioengineering9050220

Spatial Chemical Stimulation Control in Microenvironment by Microfluidic Probe Integrated Device for Cell-Based Assay
Masayuki Horayama, Kenta Shinha, Kazuya Kabayama, Teruo Fujii, Hiroshi Kimura and Dario Pisignano
PLoS ONE, 2016, 11, e0168158
DOI: 10.1371/journal.pone.0168158

Experimental models to study intestinal microbes–mucus interactions in health and disease
Lucie Etienne-Mesmin, Benoit Chassaing, Mickaël Desvaux, Kim De Paepe, Raphaële Gresse, Thomas Sauvaitre, Evelyne Forano, Tom Van de Wiele, Stephanie Schüller, Nathalie Juge and Stéphanie Blanquet-Diot
FEMS Microbiology Reviews, 2019, 43, 457
DOI: 10.1093/femsre/fuz013

Novel ex Vivo Experimental Setup to Assay the Vectorial Transepithelial Enteroendocrine Secretions of Different Intestinal Segments
Iris Ginés, Katherine Gil-Cardoso, Paula Robles, Luis Arola, Ximena Terra, Mayte Blay, Anna Ardévol and Montserrat Pinent
J. Agric. Food Chem., 2018, 66, 11622
DOI: 10.1021/acs.jafc.8b03046

GelMA as scaffold material for epithelial cells to emulate the small intestinal microenvironment
Inez Roegiers, Tom Gheysens, Manon Minsart, Pieter De Clercq, Kim Vanbeversluys, Nikoletta Rać, Grzegorz Stroka, Jana de Croock, Tom Van de Wiele, Peter Dubruel and Marta Calatayud Arroyo
Sci Rep, 2025, 15
DOI: 10.1038/s41598-024-81533-5

Intestinal therapeutic agent delivery microrobot with magnetic targeting and pH-triggered degradation abilities
Hyoryong Lee, Na Eun Mun, Deockhee Yoon, Yun-Jung Choi, Jam-Eon Park, Dong-Jae Kim, Su Woong Yoo, Seung-Hwan Park and Sukho Park
Biomaterials, 2026, 325, 123561
DOI: 10.1016/j.biomaterials.2025.123561

New Age Strategies To Reconstruct Mucosal Tissue Colonization and Growth in Cell Culture Systems
Alyssa C. Fasciano, Joan Mecsas, Ralph R. Isberg, Pascale Cossart, Craig R. Roy and Philippe Sansonetti
Microbiol Spectr, 2019, 7
DOI: 10.1128/microbiolspec.BAI-0013-2019

Recent Advances in Microfluidic-Based Microphysiological Systems
Sung-Min Kang
BioChip J, 2022, 16, 13
DOI: 10.1007/s13206-021-00043-y

Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies
Joanne M. Donkers, Jamie I. van der Vaart and Evita van de Steeg
Biomimetics, 2023, 8, 226
DOI: 10.3390/biomimetics8020226

Addressing the ADME Challenges of Compound Loss in a PDMS-Based Gut-on-Chip Microphysiological System
Patrick Carius, Ferdinand Anton Weinelt, Chris Cantow, Markus Holstein, Aaron M. Teitelbaum and Yunhai Cui
Pharmaceutics, 2024, 16, 296
DOI: 10.3390/pharmaceutics16030296

Grow with the Flow: When Morphogenesis Meets Microfluidics
Pinak Samal, Clemens van Blitterswijk, Roman Truckenmüller and Stefan Giselbrecht
Advanced Materials, 2019, 31
DOI: 10.1002/adma.201805764

Heterogenous morphogenesis of Caco‐2 cells reveals that flow induces three‐dimensional growth and maturation at high initial seeding cell densities
Asli Aybike Dogan and Martin Dufva
Biotech & Bioengineering, 2023, 120, 1667
DOI: 10.1002/bit.28362

Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women
Katja Graf, José Martin Murrieta-Coxca, Tobias Vogt, Sophie Besser, Daria Geilen, Tim Kaden, Anne-Katrin Bothe, Diana Maria Morales-Prieto, Behnam Amiri, Stephan Schaller, Ligaya Kaufmann, Martin Raasch, Ramy M. Ammar and Christian Maass
Front. Pharmacol., 2025, 16
DOI: 10.3389/fphar.2025.1528748

Diet, food components and the intestinal barrier
J. P. Gleeson
Nutrition Bulletin, 2017, 42, 123
DOI: 10.1111/nbu.12260

My cells, my model: immune-competent autologous organ-on-chip systems as a new paradigm in precision medicine
Francesco Bisconti, Hugo Abreu, Thuy Duong Nguyen, Fabiola Stolfi, Noemi Corbezzolo, Giuseppe Gigli, Davide Raineri, Giuseppe Cappellano, Alessandro Polini, Francesca Gervaso and Annalisa Chiocchetti
Front. Immunol., 2025, 16
DOI: 10.3389/fimmu.2025.1712796

Timescales of gut microbiome dynamics
Brandon H Schlomann and Raghuveer Parthasarathy
Current Opinion in Microbiology, 2019, 50, 56
DOI: 10.1016/j.mib.2019.09.011

Individualized Pharmacotherapy Utilizing Genetic Biomarkers and Novel In Vitro Systems As Predictive Tools for Optimal Drug Development and Treatment
Magnus Ingelman-Sundberg and Volker M. Lauschke
Drug Metabolism and Disposition, 2024, 52, 467
DOI: 10.1124/dmd.123.001302

Biofabrication and simulation techniques for gut-on-a-chip
Ke Wang, Yushen Wang, Junlei Han, Zhixiang Liang, Wenhong Zhang, Xinyu Li, Jun Chen and Li Wang
Biofabrication, 2025, 17, 022011
DOI: 10.1088/1758-5090/adb7c1

The 3Rs in research: a contemporary approach to replacement, reduction and refinement
Judy MacArthur Clark
Br J Nutr, 2018, 120, S1
DOI: 10.1017/S0007114517002227

Systematic comparison of transcriptomes of Caco-2 cells cultured under different cellular and physiological conditions
Janneke Elzinga, Menno Grouls, Guido J. E. J. Hooiveld, Meike van der Zande, Hauke Smidt and Hans Bouwmeester
Arch Toxicol, 2023, 97, 737
DOI: 10.1007/s00204-022-03430-y

The Role of Microfluidics for Organ on Chip Simulations
Aziz Aziz, Chunyang Geng, Mengjie Fu, Xiaohui Yu, Kairong Qin and Bo Liu
Bioengineering, 2017, 4, 39
DOI: 10.3390/bioengineering4020039

A bronchiole-on-a-chip and a stretching system for studying human disease model of asthma attack
Chun-Hui Sun, Kang-Yun Lee, Yao-Fei Chan, Shu-Chuan Ho, Chien-Ying Liu, Sheng-Ming Wu, Weilun Sun, Yi-Ting Ke and Cheng-Hsien Liu
Sensors and Actuators B: Chemical, 2024, 416, 136031
DOI: 10.1016/j.snb.2024.136031

Organs-on-a-chip: a new tool for drug discovery
Alessandro Polini, Ljupcho Prodanov, Nupura S Bhise, Vijayan Manoharan, Mehmet R Dokmeci and Ali Khademhosseini
Expert Opinion on Drug Discovery, 2014, 9, 335
DOI: 10.1517/17460441.2014.886562

Bio–Microfabrication of 2D and 3D Biomimetic Gut-on-a-Chip
Yeongseok Jang, Jinmu Jung and Jonghyun Oh
Micromachines, 2023, 14, 1736
DOI: 10.3390/mi14091736

Modeling radiation injury-induced cell death and countermeasure drug responses in a human Gut-on-a-Chip
Sasan Jalili-Firoozinezhad, Rachelle Prantil-Baun, Amanda Jiang, Ratnakar Potla, Tadanori Mammoto, James C. Weaver, Thomas C. Ferrante, Hyun Jung Kim, Joaquim M. S. Cabral, Oren Levy and Donald E. Ingber
Cell Death Dis, 2018, 9
DOI: 10.1038/s41419-018-0304-8

In VitroModels of the Small Intestine: Engineering Challenges and Engineering Solutions
Sarah A. Hewes, Reid L. Wilson, Mary K. Estes, Noah F. Shroyer, Sarah E. Blutt and K. Jane Grande-Allen
Tissue Engineering Part B: Reviews, 2020, 26, 313
DOI: 10.1089/ten.teb.2019.0334

Current Evidence on the Bioavailability of Food Bioactive Peptides
Lourdes Amigo and Blanca Hernández-Ledesma
Molecules, 2020, 25, 4479
DOI: 10.3390/molecules25194479

Probiotic Potential of Bacillus licheniformis and Bacillus pumilus Isolated from Tibetan Yaks, China
Zhibo Zeng, Jiabin Zhang, Yan Li, Kewei Li, Saisai Gong, Feiran Li, Pengpeng Wang, Mudassar Iqbal, Muhammad Fakhar-e-Alam Kulyar and Jiakui Li
Probiotics & Antimicro. Prot., 2022, 14, 579
DOI: 10.1007/s12602-022-09939-z

Drug absorption related nephrotoxicity assessment on an intestine-kidney chip
Zhongyu Li, Wentao Su, Yujuan Zhu, Tingting Tao, Dong Li, Xiaojun Peng and Jianhua Qin
Biomicrofluidics, 2017, 11
DOI: 10.1063/1.4984768

Microfluidic trends in drug screening and drug delivery
Jianguo Feng, Jiri Neuzil, Andreas Manz, Ciprian Iliescu and Pavel Neuzil
TrAC Trends in Analytical Chemistry, 2023, 158, 116821
DOI: 10.1016/j.trac.2022.116821

Epithelial models to study food allergen-induced barrier disruption and immune activation
Marija Gavrovic-Jankulovic and Linette E.M. Willemsen
Drug Discovery Today: Disease Models, 2015, 17-18, 29
DOI: 10.1016/j.ddmod.2016.09.002

Human enteroids as an ex-vivo model of host–pathogen interactions in the gastrointestinal tract
Jennifer Foulke-Abel, Julie In, Olga Kovbasnjuk, Nicholas C Zachos, Khalil Ettayebi, Sarah E Blutt, Joseph M Hyser, Xi-Lei Zeng, Sue E Crawford, James R Broughman, Mary K Estes and Mark Donowitz
Exp Biol Med (Maywood), 2014, 239, 1124
DOI: 10.1177/1535370214529398

Organ-on-Chip Models: New Opportunities for Biomedical Research
Alexander S. Mosig
Future Sci. OA, 2017, 3
DOI: 10.4155/fsoa-2016-0038

Rapid Fabrication of Membrane-Integrated Thermoplastic Elastomer Microfluidic Devices
Alexander H. McMillan, Emma K. Thomée, Alessandra Dellaquila, Hussam Nassman, Tatiana Segura and Sasha Cai Lesher-Pérez
Micromachines, 2020, 11, 731
DOI: 10.3390/mi11080731

Organ-on-a-Chip: Design and Simulation of Various Microfluidic Channel Geometries for the Influence of Fluid Dynamic Parameters
Francesca Pisapia, Wamadeva Balachandran and Manoochehr Rasekh
Applied Sciences, 2022, 12, 3829
DOI: 10.3390/app12083829

In vitro cellular model systems provide a promising alternative to animal experiments for studying the intestine-organ axis
Alessandra Vitale, Cristiana De Musis, Marida Bimonte, Josep Rubert and Vincenzo Fogliano
Critical Reviews in Biotechnology, 2025, 45, 1189
DOI: 10.1080/07388551.2025.2452620

Human Gut-On-A-Chip Supports Polarized Infection of Coxsackie B1 Virus In Vitro
Remi Villenave, Samantha Q. Wales, Tiama Hamkins-Indik, Efstathia Papafragkou, James C. Weaver, Thomas C. Ferrante, Anthony Bahinski, Christopher A. Elkins, Michael Kulka, Donald E. Ingber and Zheng Xing
PLoS ONE, 2017, 12, e0169412
DOI: 10.1371/journal.pone.0169412

Nutritional deficiency in an intestine-on-a-chip recapitulates injury hallmarks associated with environmental enteric dysfunction
Amir Bein, Cicely W. Fadel, Ben Swenor, Wuji Cao, Rani K. Powers, Diogo M. Camacho, Arash Naziripour, Andrew Parsons, Nina LoGrande, Sanjay Sharma, Seongmin Kim, Sasan Jalili-Firoozinezhad, Jennifer Grant, David T. Breault, Junaid Iqbal, Asad Ali, Lee A. Denson, Sean R. Moore, Rachelle Prantil-Baun, Girija Goyal and Donald E. Ingber
Nat. Biomed. Eng, 2022, 6, 1236
DOI: 10.1038/s41551-022-00899-x

‘Chip’-ing away at morphogenesis – application of organ-on-chip technologies to study tissue morphogenesis
Matthew J. White, Tania Singh, Eric Wang, Quinton Smith and Matthew L. Kutys
Journal of Cell Science, 2023, 136
DOI: 10.1242/jcs.261130

Human Microphysiological Models of Intestinal Tissue and Gut Microbiome
Steven N. Steinway, Jad Saleh, Bon-Kyoung Koo, Delphine Delacour and Deok-Ho Kim
Front. Bioeng. Biotechnol., 2020, 8
DOI: 10.3389/fbioe.2020.00725

In Vitro Models of Intestine Innate Immunity
Terrence T. Roh, Ying Chen, Sara Rudolph, Michelle Gee and David L. Kaplan
Trends in Biotechnology, 2021, 39, 274
DOI: 10.1016/j.tibtech.2020.07.009

What can microfluidics do for human microbiome research?
Hsih-Yin Tan and Yi-Chin Toh
Biomicrofluidics, 2020, 14
DOI: 10.1063/5.0012185

Organ-on-a-Chip Models for Studying Gut–Brain Axis and Appetite Regulation
Sehoon Jeong and Sunhye Lee
BioChip J, 2026, 20, 1
DOI: 10.1007/s13206-025-00243-w

Modelling cancer in microfluidic human organs-on-chips
Alexandra Sontheimer-Phelps, Bryan A. Hassell and Donald E. Ingber
Nat Rev Cancer, 2019, 19, 65
DOI: 10.1038/s41568-018-0104-6

The impact of microfluidics in high-throughput drug-screening applications
Paola De Stefano, Elena Bianchi and Gabriele Dubini
Biomicrofluidics, 2022, 16
DOI: 10.1063/5.0087294

Advancement of MPS Research for Animal Alternatives in Pharmaceutical Industry
Tomoki Imaoka and Wataru Obuchi
DDS, 2025, 40, 254
DOI: 10.2745/dds.40.254

Modeling Clostridioides difficile toxin pathogenesis and antiserum protection in an immunocompetent intestine-on-chip platform
Valentin D. Wegner, Maria Warschinke, Ibtissem Ben Brahim, Adrian Feile, Karen E. Huber and Alexander S. Mosig
Sci Rep, 2026, 16
DOI: 10.1038/s41598-026-44170-8

Gut‐on‐a‐chip platforms: Bridging in vitro and in vivo models for advanced gastrointestinal research
Awurama Ofori‐Kwafo, Indira Sigdel, Earshed Al Mamun, Jasenka Zubcevic and Yuan Tang
Physiological Reports, 2025, 13
DOI: 10.14814/phy2.70356

Precision-cut intestinal slices: alternative model for drug transport, metabolism, and toxicology research
Ming Li, Inge A. M. de Graaf and Geny M. M. Groothuis
Expert Opinion on Drug Metabolism & Toxicology, 2016, 12, 175
DOI: 10.1517/17425255.2016.1125882

In vitro hepatic steatosis model based on gut–liver‐on‐a‐chip
Joong‐won Jeon, Seung Hwan Lee, Donghyun Kim and Jong Hwan Sung
Biotechnology Progress, 2021, 37
DOI: 10.1002/btpr.3121

Gastrointestinal organoids in the study of viral infections
J. Thomas Gebert, Francesca Scribano, Kristen A. Engevik, Jacob L. Perry and Joseph M. Hyser
American Journal of Physiology-Gastrointestinal and Liver Physiology, 2023, 324, G51
DOI: 10.1152/ajpgi.00152.2022

Thread‐Like Radical‐Polymerization via Autonomously Propelled (TRAP) Bots
Sarvesh Kumar Srivastava, Fatemeh Ajalloueian and Anja Boisen
Advanced Materials, 2019, 31
DOI: 10.1002/adma.201901573

Gut-on-a-chip for disease models
Changxiu Xian, Jiaxin Zhang, Suqing Zhao and Xiang-Guang Li
J Tissue Eng, 2023, 14
DOI: 10.1177/20417314221149882

Microfluidic Organ-on-a-Chip Models of Human Intestine
Amir Bein, Woojung Shin, Sasan Jalili-Firoozinezhad, Min Hee Park, Alexandra Sontheimer-Phelps, Alessio Tovaglieri, Angeliki Chalkiadaki, Hyun Jung Kim and Donald E. Ingber
Cellular and Molecular Gastroenterology and Hepatology, 2018, 5, 659
DOI: 10.1016/j.jcmgh.2017.12.010

Multi-Compartment 3D-Cultured Organ-on-a-Chip: Towards a Biomimetic Lymph Node for Drug Development
Aya Shanti, Bisan Samara, Amal Abdullah, Nicholas Hallfors, Dino Accoto, Jiranuwat Sapudom, Aseel Alatoom, Jeremy Teo, Serena Danti and Cesare Stefanini
Pharmaceutics, 2020, 12, 464
DOI: 10.3390/pharmaceutics12050464

A highly stable, pressure-driven, flow control system based on Coriolis mass flow sensors for organs-on-chips
Pim de Haan, Jean-Paul S.H. Mulder, Joost C. Lötters and Elisabeth Verpoorte
Flow Measurement and Instrumentation, 2024, 97, 102576
DOI: 10.1016/j.flowmeasinst.2024.102576

Biomechanical Strain Exacerbates Inflammation on a Progeria‐on‐a‐Chip Model
João Ribas, Yu Shrike Zhang, Patrícia R. Pitrez, Jeroen Leijten, Mario Miscuglio, Jeroen Rouwkema, Mehmet Remzi Dokmeci, Xavier Nissan, Lino Ferreira and Ali Khademhosseini
Small, 2017, 13
DOI: 10.1002/smll.201603737

Gradients in the in vivo intestinal stem cell compartment and their in vitro recapitulation in mimetic platforms
Sigita Malijauskaite, Sinead Connolly, David Newport and Kieran McGourty
Cytokine & Growth Factor Reviews, 2021, 60, 76
DOI: 10.1016/j.cytogfr.2021.03.002

Intestine‐on‐a‐chip for intestinal disease study and pharmacological research
Dongxue Zhang and Liang Qiao
VIEW, 2023, 4
DOI: 10.1002/VIW.20220037

Microfluidic Gastrointestinal Cell Culture Technologies—Improvements in the Past Decade
Adrian J. T. Teo, Siu-Kin Ng, Kaydeson Khoo, Sunny Hei Wong and King Ho Holden Li
Biosensors, 2024, 14, 449
DOI: 10.3390/bios14090449

Intestinal organ chips for disease modelling and personalized medicine
Alican Özkan, Nina Teresa LoGrande, Jessica F. Feitor, Girija Goyal and Donald E. Ingber
Nat Rev Gastroenterol Hepatol, 2024, 21, 751
DOI: 10.1038/s41575-024-00968-3

Engineering biomimetic tissue barrier models on chips: From design and fabrication to applications in disease modeling and drug screening
Rohollah Nasiri, Masoud Madadelahi, Mohammad Reza Nikmaneshi, Begum Gokce, Mohamad Ali Bijarchi, Shilp Shah, Zuzana Tirpáková, Dirkje Van Gastel, Nayere Taebnia, Natan Roberto de Barros, Yangzhi Zhu, Zehra Gul Morcimen, Baris Gulicli, Rouhollah Habibey, Aylin Sendemir, Saumey Jain, Alessandro Enrico, Volker M. Lauschke, Mehmet Remzi Dokmeci, Guillem Pratx, Ali Khademhosseini and Anna Herland
Biomaterials, 2026, 327, 123739
DOI: 10.1016/j.biomaterials.2025.123739

A Review of the Application of Body-on-a-Chip for Drug Test and Its Latest Trend of Incorporating Barrier Tissue
Haoyi Jin and Yanqiu Yu
SLAS Technology, 2016, 21, 615
DOI: 10.1177/2211068215619126

Application of Microphysiological Systems to Enhance Safety Assessment in Drug Discovery
Lorna Ewart, Eva-Maria Dehne, Kristin Fabre, Susan Gibbs, James Hickman, Ellinor Hornberg, Magnus Ingelman-Sundberg, Kyung-Jin Jang, David R. Jones, Volker M. Lauschke, Uwe Marx, Jerome T. Mettetal, Amy Pointon, Dominic Williams, Wolfram-Hubertus Zimmermann and Peter Newham
Annu. Rev. Pharmacol. Toxicol., 2018, 58, 65
DOI: 10.1146/annurev-pharmtox-010617-052722

Organ‐on‐a‐Chip Technology for Reproducing Multiorgan Physiology
Seung Hwan Lee and Jong Hwan Sung
Adv Healthcare Materials, 2018, 7
DOI: 10.1002/adhm.201700419

Bedside to bench: the outlook for psychedelic research
Victor P. Acero, Emily S. Cribas, Kevin D. Browne, Olivia Rivellini, Justin C. Burrell, John C. O’Donnell, Suradip Das and D. Kacy Cullen
Front. Pharmacol., 2023, 14
DOI: 10.3389/fphar.2023.1240295

The Use of Defined Microbial Communities To Model Host-Microbe Interactions in the Human Gut
Janneke Elzinga, John van der Oost, Willem M. de Vos and Hauke Smidt
Microbiol Mol Biol Rev, 2019, 83
DOI: 10.1128/MMBR.00054-18

An Insight on Microfluidic Organ-on-a-Chip Models for PM2.5-Induced Pulmonary Complications
Disha Shah, Bhavarth Dave, Mehul R. Chorawala, Bhupendra G. Prajapati, Sudarshan Singh, Gehan M. Elossaily, Mohd Nazam Ansari and Nemat Ali
ACS Omega, 2024, 9, 13534
DOI: 10.1021/acsomega.3c10271

Head-to-Head Comparison of Caco-2 Transwell and Gut-on-a-Chip Models for Assessing Oral Peptide Formulations
John P. Gleeson, Stephanie Y. Zhang, Natalia Subelzu, Jing Ling, Becky Nissley, Whitney Ong, Rebecca Nofsinger and Filippos Kesisoglou
Mol. Pharmaceutics, 2024, 21, 3880
DOI: 10.1021/acs.molpharmaceut.4c00210

Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection
Kunal Sharma, Neeraj Dhar, Vivek V Thacker, Thomas M Simonet, Francois Signorino-Gelo, Graham W Knott and John D McKinney
eLife, 2021, 10
DOI: 10.7554/eLife.66481

Oral mucosa-on-a-chip to assess layer-specific responses to bacteria and dental materials
Christopher Rahimi, Benjamin Rahimi, Dominic Padova, Seyed A. Rooholghodos, Diane R. Bienek, Xiaolong Luo, Gili Kaufman and Christopher B. Raub
Biomicrofluidics, 2018, 12
DOI: 10.1063/1.5048938

Next-generation non-animal models for inflammatory bowel disease: In vitro and in silico approaches for mechanistic understanding
Priyanka Raju Chougule and Sukesh Narayan Sinha
NAM Journal, 2025, 1, 100051
DOI: 10.1016/j.namjnl.2025.100051

Advances in reconstructing intestinal functionalities in vitro: From two/three dimensional-cell culture platforms to human intestine-on-a-chip
Li Wang, Jian Wu, Jun Chen, Wenkun Dou, Qili Zhao, Junlei Han, Jinliang Liu, Weiguang Su, Anqing Li, Pengbo Liu, Zhao An, Chonghai Xu and Yu Sun
Talanta, 2021, 226, 122097
DOI: 10.1016/j.talanta.2021.122097

A Bioprinted Tubular Intestine Model Using a Colon‐Specific Extracellular Matrix Bioink
Hohyeon Han, Yejin Park, Yoo‐mi Choi, Uijung Yong, Byeongmin Kang, Woojung Shin, Soyoun Min, Hyun Jung Kim and Jinah Jang
Adv Healthcare Materials, 2022, 11
DOI: 10.1002/adhm.202101768

Role of gut microbiota and nutrients in amyloid formation and pathogenesis of Alzheimer disease
Francesca Pistollato, Sandra Sumalla Cano, Iñaki Elio, Manuel Masias Vergara, Francesca Giampieri and Maurizio Battino
Nutr Rev, 2016, 74, 624
DOI: 10.1093/nutrit/nuw023

Biomimetic human small muscular pulmonary arteries
Qianru Jin, Anil Bhatta, Jayson V. Pagaduan, Xing Chen, Hoku West-Foyle, Jiayu Liu, Annie Hou, Dan Berkowitz, Scot C. Kuo, Frederic B. Askin, Thao D. Nguyen, David H. Gracias and Lewis H. Romer
Sci. Adv., 2020, 6
DOI: 10.1126/sciadv.aaz2598

Developing sensor materials for screening intestinal diseases
Cheng Zhao, Xiaoxuan Zhang, Guopu Chen and Luoran Shang
Mater. Futures, 2022, 1, 022401
DOI: 10.1088/2752-5724/ac48a3

Construction, Features and Regulatory Aspects of Organ-chip for Drug Delivery Applications: Advances and Prospective
Babita Gupta, Rishabha Malviya, Saurabh Srivastava, Irfan Ahmad, Safia Obaidur Rab and Prerna Uniyal
CPD, 2024, 30, 1952
DOI: 10.2174/0113816128305296240523112043

Life in 3D is never flat: 3D models to optimise drug delivery
Kathleen A. Fitzgerald, Meenakshi Malhotra, Caroline M. Curtin, Fergal J. O' Brien and Caitriona M. O' Driscoll
Journal of Controlled Release, 2015, 215, 39
DOI: 10.1016/j.jconrel.2015.07.020

Organotypic cultures as aging associated disease models
Martina M. Sanchez, Isabella A. Bagdasarian, William Darch and Joshua T. Morgan
Aging, 2022, 14, 9338
DOI: 10.18632/aging.204361

Organoids and organ-on-chip technology for investigating host-microorganism interactions
Remigiusz Walocha, MinHee Kim, Jérôme Wong-Ng, Samy Gobaa and Nathalie Sauvonnet
Microbes and Infection, 2024, 26, 105319
DOI: 10.1016/j.micinf.2024.105319

Enhanced predictive capacity using dual-parameter chip model that simulates physiological skin irritation
Byoungjun Jeon, GeonHui Lee, Maierdanjiang Wufuer, Yan Huang, Yunhee Choi, Sungwan Kim and Tae Hyun Choi
Toxicology in Vitro, 2020, 68, 104955
DOI: 10.1016/j.tiv.2020.104955

Engineering Cell Instructive Microenvironments for In Vitro Replication of Functional Barrier Organs
Francesco Urciuolo, Giorgia Imparato and Paolo Antonio Netti
Adv Healthcare Materials, 2024, 13
DOI: 10.1002/adhm.202400357

Patient-derived small intestinal myofibroblasts direct perfused, physiologically responsive capillary development in a microfluidic Gut-on-a-Chip Model
Kristen M. Seiler, Adam Bajinting, David M. Alvarado, Mahama A. Traore, Michael M. Binkley, William H. Goo, Wyatt E. Lanik, Jocelyn Ou, Usama Ismail, Micah Iticovici, Cristi R. King, Kelli L. VanDussen, Elzbieta A. Swietlicki, Vered Gazit, Jun Guo, Cliff J. Luke, Thaddeus Stappenbeck, Matthew A. Ciorba, Steven C. George, J. Mark Meacham, Deborah C. Rubin, Misty Good and Brad W. Warner
Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-60672-5

Gutsy science: In vitro systems of the human intestine to model oral drug disposition
Christopher M. Arian, Tomoki Imaoka, Jade Yang, Edward J. Kelly and Kenneth E. Thummel
Pharmacology & Therapeutics, 2022, 230, 107962
DOI: 10.1016/j.pharmthera.2021.107962

Building Blood Vessel Chips with Enhanced Physiological Relevance
Xuan Mu, Marie Denise Gerhard‐Herman and Yu Shrike Zhang
Adv Materials Technologies, 2023, 8
DOI: 10.1002/admt.202201778

Comparative Analysis of Intestinal Tract Models
C.F. Williams, G.E. Walton, L. Jiang, S. Plummer, I. Garaiova and G.R. Gibson
Annu. Rev. Food Sci. Technol., 2015, 6, 329
DOI: 10.1146/annurev-food-022814-015429

Engineering the Future of Regenerative Medicines in Gut Health with Stem Cell-Derived Intestinal Organoids
Dinesh Kumar, Sonia Gupta, Vrinda Gupta, Rajni Tanwar and Anchal Chandel
Stem Cell Rev and Rep, 2025, 21, 1449
DOI: 10.1007/s12015-025-10893-w

Microphysiological Systems (MPSs) based on Microfluidics as a Platform for Drug Discovery
Hiroshi Kimura
DDS, 2019, 34, 243
DOI: 10.2745/dds.34.243

Improvement of paracellular transport in the Caco-2 drug screening model using protein-engineered substrates
Rebecca L. DiMarco, Daniel R. Hunt, Ruby E. Dewi and Sarah C. Heilshorn
Biomaterials, 2017, 129, 152
DOI: 10.1016/j.biomaterials.2017.03.023

Skin-on-a-chip models: General overview and future perspectives
I. Risueño, L. Valencia, J. L. Jorcano and D. Velasco
APL Bioengineering, 2021, 5
DOI: 10.1063/5.0046376

Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Guoyou Huang, Fei Li, Xin Zhao, Yufei Ma, Yuhui Li, Min Lin, Guorui Jin, Tian Jian Lu, Guy M. Genin and Feng Xu
Chem. Rev., 2017, 117, 12764
DOI: 10.1021/acs.chemrev.7b00094

Engineering bacteria for diagnostic and therapeutic applications
David T. Riglar and Pamela A. Silver
Nat Rev Microbiol, 2018, 16, 214
DOI: 10.1038/nrmicro.2017.172

Engineering Micro–Nanomaterials for Biomedical Translation
Yaping Chen, Maria Alba, Terence Tieu, Ziqiu Tong, Rajpreet Singh Minhas, David Rudd, Nicolas H. Voelcker, Anna Cifuentes-Rius and Roey Elnathan
Advanced NanoBiomed Research, 2021, 1
DOI: 10.1002/anbr.202100002

The translational roadmap of the gut models, focusing on gut-on-chip
Giulia Malaguarnera, Miriam Graute and Antoni Homs Corbera
Open Res Europe, 2023, 1, 62
DOI: 10.12688/openreseurope.13709.2

Organ-on-Chip Technology for Aerobic Intestinal Host – Anaerobic Microbiota Research
Elsbeth G.B.M. Bossink, Loes I. Segerink and Mathieu Odijk
Organs-on-a-Chip, 2022, 4, 100013
DOI: 10.1016/j.ooc.2021.100013

Effect of microbubble-assisted gemcitabine delivery with repeated ultrasound exposure in a pancreatic cancer organ-on-a-chip model
Delanyo Kpeglo, Malcolm Haddrick, Margaret A. Knowles, Stephen D. Evans and Sally A. Peyman
Sci Rep, 2025, 15
DOI: 10.1038/s41598-025-30612-2

Microphysiological Systems: Next Generation Systems for Assessing Toxicity and Therapeutic Effects of Nanomaterials
Nureddin Ashammakhi, Mohammad Ali Darabi, Betül Çelebi‐Saltik, Rumeysa Tutar, Martin C. Hartel, Junmin Lee, Saber M. Hussein, Marcus J. Goudie, Mercedes Brianna Cornelius, Mehmet R. Dokmeci and Ali Khademhosseini
Small Methods, 2020, 4
DOI: 10.1002/smtd.201900589

Organoids and Bioengineered Intestinal Models: Potential Solutions to the Cryptosporidium Culturing Dilemma
Samantha Gunasekera, Alireza Zahedi, Mark O’Dea, Brendon King, Paul Monis, Benjamin Thierry, Jillian M. Carr and Una Ryan
Microorganisms, 2020, 8, 715
DOI: 10.3390/microorganisms8050715

Organ-on-a-chip engineering: Toward bridging the gap between lab and industry
Qasem Ramadan and Mohammed Zourob
Biomicrofluidics, 2020, 14
DOI: 10.1063/5.0011583

Organ-on-a-chip platforms for accelerating the evaluation of nanomedicine
Xi Chen, Yu Shrike Zhang, Xinping Zhang and Changsheng Liu
Bioactive Materials, 2021, 6, 1012
DOI: 10.1016/j.bioactmat.2020.09.022

Engineered In Vitro Disease Models
Kambez H. Benam, Stephanie Dauth, Bryan Hassell, Anna Herland, Abhishek Jain, Kyung-Jin Jang, Katia Karalis, Hyun Jung Kim, Luke MacQueen, Roza Mahmoodian, Samira Musah, Yu-suke Torisawa, Andries D. van der Meer, Remi Villenave, Moran Yadid, Kevin K. Parker and Donald E. Ingber
Annu. Rev. Pathol. Mech. Dis., 2015, 10, 195
DOI: 10.1146/annurev-pathol-012414-040418

3D stromal tissue equivalent affects intestinal epithelium morphogenesis in vitro
Vincenza De Gregorio, Giorgia Imparato, Francesco Urciuolo and Paolo A. Netti
Biotech & Bioengineering, 2018, 115, 1062
DOI: 10.1002/bit.26522

The Gut–Organ-Axis Concept: Advances the Application of Gut-on-Chip Technology
Yuxi Guo, Xuefeng Chen, Pin Gong, Guoliang Li, Wenbo Yao and Wenjuan Yang
IJMS, 2023, 24, 4089
DOI: 10.3390/ijms24044089

Pharma challenges to adoption of microphysiological system in drug research and development, especially safety assessment
Hitoshi Naraoka
Folia Pharmacol. Jpn., 2023, 158, 187
DOI: 10.1254/fpj.22116

Effects of food‐borne nanomaterials on gastrointestinal tissues and microbiota
Hans Bouwmeester, Meike van der Zande and Mark A. Jepson
WIREs Nanomed Nanobiotechnol, 2018, 10
DOI: 10.1002/wnan.1481

Mucus blocks probiotics but increases penetration of motile pathogens and induces TNF‐α and IL‐8 secretion
Abhinav Sharma, Vishnu Raman, Jungwoo Lee and Neil S. Forbes
Biotech & Bioengineering, 2020, 117, 2540
DOI: 10.1002/bit.27383

Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis
Adi Egozi, Oluwabunmi Olaloye, Lael Werner, Tatiana Silva, Blake McCourt, Richard W. Pierce, Xiaojing An, Fujing Wang, Kong Chen, Jordan S. Pober, Dror Shouval, Shalev Itzkovitz, Liza Konnikova and Ken Cadwell
PLoS Biol, 2023, 21, e3002124
DOI: 10.1371/journal.pbio.3002124

Mimicking Epithelial Tissues in Three-Dimensional Cell Culture Models
Núria Torras, María García-Díaz, Vanesa Fernández-Majada and Elena Martínez
Front. Bioeng. Biotechnol., 2018, 6
DOI: 10.3389/fbioe.2018.00197

Advancing intestinal disease research using gut-on-a-chip
Fuki Yokoi, Sayaka Deguchi and Kazuo Takayama
Regenerative Therapy, 2025, 29, 541
DOI: 10.1016/j.reth.2025.04.023

Modelling Human Gut‐Microbiome Interactions in a 3D Bioelectronic Platform
Chrysanthi‐Maria Moysidou, Douglas C. van Niekerk, Verena Stoeger, Charalampos Pitsalidis, Lorraine A. Draper, Aimee M. Withers, Katherine Hughes, Reece McCoy, Rachana Acharya, Colin Hill and Róisín M. Owens
Small Science, 2024, 4
DOI: 10.1002/smsc.202300349

In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment
Peter J. Attayek, Asad A. Ahmad, Yuli Wang, Ian Williamson, Christopher E. Sims, Scott T. Magness, Nancy L. Allbritton and Chunming Liu
PLoS ONE, 2016, 11, e0153795
DOI: 10.1371/journal.pone.0153795

Microfluidics meets metabolomics to reveal the impact of Campylobacter jejuni infection on biochemical pathways
Ninell P. Mortensen, Kelly A. Mercier, Susan McRitchie, Tammy B. Cavallo, Wimal Pathmasiri, Delisha Stewart and Susan J. Sumner
Biomed Microdevices, 2016, 18
DOI: 10.1007/s10544-016-0076-9

Organs-on-a-Chip Module: A Review from the Development and Applications Perspective
Juan Eduardo Sosa-Hernández, Angel M. Villalba-Rodríguez, Kenya D. Romero-Castillo, Mauricio A. Aguilar-Aguila-Isaías, Isaac E. García-Reyes, Arturo Hernández-Antonio, Ishtiaq Ahmed, Ashutosh Sharma, Roberto Parra-Saldívar and Hafiz M. N. Iqbal
Micromachines, 2018, 9, 536
DOI: 10.3390/mi9100536

Statin induces epithelial cell death and tissue barrier damage in renal tubule on chip
Shiquan Wang, Yaqi Liu, Daqing Wang, Xiaoqing Zhang, Hyon U. Pak, Jianhua Qin and Xu Zhang
Toxicology, 2026, 519, 154309
DOI: 10.1016/j.tox.2025.154309

Microfluidic organ-on-chip systems for periodontal research: advances and future directions
Gopu Sriram and Hardik Makkar
Front. Bioeng. Biotechnol., 2025, 12
DOI: 10.3389/fbioe.2024.1490453

Porcine intestinal organoids cultured in an organ-on-a-chip microphysiological system
James Bacon, Halie Kitchel, John Stutz, Jack Jia Hua Chen, Aaron Smith, Robert D. Van Horn, Christopher Moreland, Trent Abraham, Thomas Baker, Eitaro Aihara and Kathleen Hillgren
Biochemistry and Biophysics Reports, 2025, 42, 102036
DOI: 10.1016/j.bbrep.2025.102036

Bioengineering approaches to simulate human colon microbiome ecosystem
Dalila Roupar, Paulo Berni, Joana T. Martins, Ana C. Caetano, José A. Teixeira and Clarisse Nobre
Trends in Food Science & Technology, 2021, 112, 808
DOI: 10.1016/j.tifs.2021.04.035

From Microscale Devices to 3D Printing
Anton V. Borovjagin, Brenda M. Ogle, Joel L. Berry and Jianyi Zhang
Circulation Research, 2017, 120, 150
DOI: 10.1161/CIRCRESAHA.116.308538

3D polyelectrolyte scaffolds to mimic exocrine glands: a step towards a prostate-on-chip platform
Nathalie Picollet-D’hahan, Axel Tollance, Cristina Belda Marin, Lavinia Liguori, Christophe Marquette, Odile Filhol-Cochet, Isabelle Vilgrain, Guillaume Laffitte, Florence Rivera, Jean-Pierre Alcaraz, Jacques Thélu, Olivier Nicoud, Thibaud Moufle-Milot, Maxime Legues, Ali Bouamrani, Adrien Mombrun, Benoit Gilquin, Sophie Gerbaud, Patricia Obeid, Fréderique Kermarrec, Xavier Gidrol and Donald K. Martin
The EuroBiotech Journal, 2018, 2, 180
DOI: 10.2478/ebtj-2018-0048

Blood and Lymphatic Vasculatures On-Chip Platforms and Their Applications for Organ-Specific In Vitro Modeling
Aria R. Henderson, Hyoann Choi and Esak Lee
Micromachines, 2020, 11, 147
DOI: 10.3390/mi11020147

Design of 3D printed insert for hanging culture of Caco-2 cells
Chong Shen, Qin Meng and Guoliang Zhang
Biofabrication, 2014, 7, 015003
DOI: 10.1088/1758-5090/7/1/015003

Current Perspectives on Gastrointestinal Models to Assess Probiotic-Pathogen Interactions
Mehreen Anjum, Arja Laitila, Arthur C. Ouwehand and Sofia D. Forssten
Front. Microbiol., 2022, 13
DOI: 10.3389/fmicb.2022.831455

Modeling Host-Pathogen Interactions in the Context of the Microenvironment: Three-Dimensional Cell Culture Comes of Age
Jennifer Barrila, Aurélie Crabbé, Jiseon Yang, Karla Franco, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Sandhya Gangaraju, C. Mark Ott, Carolyn B. Coyne, Mina J. Bissell, Cheryl A. Nickerson and Anthony T. Maurelli
Infect Immun, 2018, 86
DOI: 10.1128/IAI.00282-18

Environmental toxicology wars: Organ-on-a-chip for assessing the toxicity of environmental pollutants
Sheng Yang, Zaozao Chen, Yanping Cheng, Tong Liu, Lihong Yin, Yuepu Pu and Geyu Liang
Environmental Pollution, 2021, 268, 115861
DOI: 10.1016/j.envpol.2020.115861

Orally ingestible medical devices for gut engineering
Nikolaj Kofoed Mandsberg, Juliane Fjelrad Christfort, Khorshid Kamguyan, Anja Boisen and Sarvesh Kumar Srivastava
Advanced Drug Delivery Reviews, 2020, 165-166, 142
DOI: 10.1016/j.addr.2020.05.004

Pathology, proteomics and the pathway to personalised medicine
Ping Jin, Jiang Lan, Kui Wang, Mark S. Baker, Canhua Huang and Edouard C. Nice
Expert Review of Proteomics, 2018, 15, 231
DOI: 10.1080/14789450.2018.1425618

Bioengineering and regeneration of gastrointestinal tissue: where are we now and what comes next?
Elie Zakhem, Shreya Raghavan, Riley A. Suhar and Khalil N. Bitar
Expert Opinion on Biological Therapy, 2019, 19, 527
DOI: 10.1080/14712598.2019.1595579

Effect of mechanical forces on cellular response to radiation
Jerome Lacombe and Frederic Zenhausern
Radiotherapy and Oncology, 2022, 176, 187
DOI: 10.1016/j.radonc.2022.10.006

Architecture in 3D cell culture: An essential feature for in vitro toxicology
Sophie A. Lelièvre, Tim Kwok and Shirisha Chittiboyina
Toxicology in Vitro, 2017, 45, 287
DOI: 10.1016/j.tiv.2017.03.012

The small intestine: dining table of host–microbiota meetings
Karen Delbaere, Inez Roegiers, Auriane Bron, Claude Durif, Tom Van de Wiele, Stéphanie Blanquet-Diot and Ludovica Marinelli
FEMS Microbiology Reviews, 2023, 47
DOI: 10.1093/femsre/fuad022

An anaerobic in vitro flow model for studying interactions at the gastrointestinal host–microbe interface
L. L. Bang, J. S. Pettersen, N. Høiland, A. M. Rojek, D. R. Tornby, J. Møller-Jensen, U. S. Justesen, R. M. Pedersen and T. E. Andersen
npj Biofilms Microbiomes, 2025, 11
DOI: 10.1038/s41522-025-00800-z

Advanced Organ-on-a-Chip Devices to Investigate Liver Multi-Organ Communication: Focus on Gut, Microbiota and Brain
Lucia Boeri, Luca Izzo, Lorenzo Sardelli, Marta Tunesi, Diego Albani and Carmen Giordano
Bioengineering, 2019, 6, 91
DOI: 10.3390/bioengineering6040091

Engineering organ-on-a-chip systems to model viral infections
Fahimeh Shahabipour, Sandro Satta, Mahboobeh Mahmoodi, Argus Sun, Natan Roberto de Barros, Song Li, Tzung Hsiai and Nureddin Ashammakhi
Biofabrication, 2023, 15, 022001
DOI: 10.1088/1758-5090/ac6538

In vitro immunity: an overview of immunocompetent organ-on-chip models
Andrew I. Morrison, Mirthe J. Sjoerds, Leander A. Vonk, Susan Gibbs and Jasper J. Koning
Front. Immunol., 2024, 15
DOI: 10.3389/fimmu.2024.1373186

Is bacterial vaginosis a disease?
Gregor Reid
Appl Microbiol Biotechnol, 2018, 102, 553
DOI: 10.1007/s00253-017-8659-9

Human Organoids and Organs‐on‐Chips for Addressing COVID‐19 Challenges
Yaqing Wang, Peng Wang and Jianhua Qin
Advanced Science, 2022, 9
DOI: 10.1002/advs.202105187

In Vitro Intestinal and Liver Models for Toxicity Testing
Sophia M. Orbach, Rebekah R. Less, Anjaney Kothari and Padmavathy Rajagopalan
ACS Biomater. Sci. Eng., 2017, 3, 1898
DOI: 10.1021/acsbiomaterials.6b00699

Human Organs‐on‐Chips: A Review of the State‐of‐the‐Art, Current Prospects, and Future Challenges
Payam Zarrintaj, Mohammad Reza Saeb, Florian J. Stadler, Mohsen Khodadadi Yazdi, Mojtaba Nasiri Nezhad, Shabnam Mohebbi, Farzad Seidi, Mohammad Reza Ganjali and Masoud Mozafari
Advanced Biology, 2022, 6
DOI: 10.1002/adbi.202000526

Human intestinal models to study interactions between intestine and microbes
Arturo Aguilar-Rojas, Jean-Christophe Olivo-Marin and Nancy Guillen
Open Biology, 2020, 10
DOI: 10.1098/rsob.200199

Physiological Shear Stress Enhances Differentiation, Mucus-Formation and Structural 3D Organization of Intestinal Epithelial Cells In Vitro
Marcus Lindner, Anna Laporte, Stephan Block, Laura Elomaa and Marie Weinhart
Cells, 2021, 10, 2062
DOI: 10.3390/cells10082062

Unraveling Caco-2 cells through functional and transcriptomic assessments
Ye Eun Jeong, Katherine Shea and Kevin A. Ford
Regulatory Toxicology and Pharmacology, 2025, 156, 105771
DOI: 10.1016/j.yrtph.2025.105771

Novel Microfluidic Colon with an Extracellular Matrix Membrane
Chengyao Wang, Nida Tanataweethum, Sonali Karnik and Abhinav Bhushan
ACS Biomater. Sci. Eng., 2018, 4, 1377
DOI: 10.1021/acsbiomaterials.7b00883

Mechano-Transduction: From Molecules to Tissues
Beth L. Pruitt, Alexander R. Dunn, William I. Weis and W. James Nelson
PLoS Biol, 2014, 12, e1001996
DOI: 10.1371/journal.pbio.1001996

The Gut-Brain Axis in Neurodegenerative Diseases and Relevance of the Canine Model: A Review
Yoko M. Ambrosini, Dana Borcherding, Anumantha Kanthasamy, Hyun Jung Kim, Auriel A. Willette, Albert Jergens, Karin Allenspach and Jonathan P. Mochel
Front. Aging Neurosci., 2019, 11
DOI: 10.3389/fnagi.2019.00130

Oral uptake of nanoparticles: human relevance and the role of in vitro systems
Eleonore Fröhlich and Eva Roblegg
Arch Toxicol, 2016, 90, 2297
DOI: 10.1007/s00204-016-1765-0

Characterization of an engineered live bacterial therapeutic for the treatment of phenylketonuria in a human gut-on-a-chip
M. Tyler Nelson, Mark R. Charbonneau, Heidi G. Coia, Mary J. Castillo, Corey Holt, Eric S. Greenwood, Peter J. Robinson, Elaine A. Merrill, David Lubkowicz and Camilla A. Mauzy
Nat Commun, 2021, 12
DOI: 10.1038/s41467-021-23072-5

Microengineered Organ-on-a-chip Platforms towards Personalized Medicine
Ranjith Kumar Kankala, Shi-Bin Wang and Ai-Zheng Chen
CPD, 2019, 24, 5354
DOI: 10.2174/1381612825666190222143542

An organotypic slice model for ex vivo study of neural, immune, and microbial interactions of mouse intestine
Luke A. Schwerdtfeger, Elizabeth P. Ryan and Stuart A. Tobet
American Journal of Physiology-Gastrointestinal and Liver Physiology, 2016, 310, G240
DOI: 10.1152/ajpgi.00299.2015

Tissue Engineering Laboratory Models of the Small Intestine
Rasha Hatem Dosh, Nicola Jordan-Mahy, Christopher Sammon and Christine Lyn Le Maitre
Tissue Engineering Part B: Reviews, 2018, 24, 98
DOI: 10.1089/ten.teb.2017.0276

In silico modelling of organ-on-a-chip devices: an overview
Yue Wang, Lucia Marucci and Martin E. Homer
Front. Bioeng. Biotechnol., 2025, 12
DOI: 10.3389/fbioe.2024.1520795

Organs-on-a-Chip: A Fast Track for Engineered Human Tissues in Drug Development
Kacey Ronaldson-Bouchard and Gordana Vunjak-Novakovic
Cell Stem Cell, 2018, 22, 310
DOI: 10.1016/j.stem.2018.02.011

Microphysiological gut-on-chip enables extended in vitro development of Cryptosporidium hominis
Samantha Gunasekera, Benjamin Thierry, Brendon King, Paul Monis, Jillian M. Carr, Abha Chopra, Mark Watson, Mark O’Dea, Edward Cheah, Ramesh Ram, Peta L. Clode, Nawal Hijjawi and Una Ryan
Front. Cell. Infect. Microbiol., 2025, 15
DOI: 10.3389/fcimb.2025.1564806

Advanced strategies in organoid/organ-on-a-chip for food safety and nutrition
Yiyan Zhou, Feiran Wang, Rui Zhao, Tianyi Huang, Wentao Su, Ya Wen, Zhixian He and Dong Li
Food Research International, 2025, 219, 117011
DOI: 10.1016/j.foodres.2025.117011

Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system
Marie Shinohara, Hiroshi Arakawa, Yuuichi Oda, Nobuaki Shiraki, Shinji Sugiura, Takumi Nishiuchi, Taku Satoh, Keita Iino, Sylvia Leo, Yusuke Kato, Karin Araya, Takumi Kawanishi, Tomoki Nakatsuji, Manami Mitsuta, Kosuke Inamura, Tomomi Goto, Kenta Shinha, Wataru Nihei, Kikuo Komori, Masaki Nishikawa, Shoen Kume, Yukio Kato, Toshiyuki Kanamori, Yasuyuki Sakai and Hiroshi Kimura
Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-84861-y

Biomaterials meet organ-on-chips – a perspective on tumor modeling
Cátia F. Monteiro, Inês A. Deus, Catarina A. Custódio and João F. Mano
International Materials Reviews, 2025, 70, 31
DOI: 10.1177/09506608241303436

Microphysiological Engineering of Immune Responses in Intestinal Inflammation
Yoko M. Ambrosini, Woojung Shin, Soyoun Min and Hyun Jung Kim
Immune Netw, 2020, 20
DOI: 10.4110/in.2020.20.e13

Microfluidic gut-on-a-chip with three-dimensional villi structure
Kyu-Young Shim, Dongwook Lee, Jeonghun Han, Nam-Trung Nguyen, Sungsu Park and Jong Hwan Sung
Biomed Microdevices, 2017, 19
DOI: 10.1007/s10544-017-0179-y

Cytokine induced inflammatory bowel disease model using organ-on-a-chip technology
Christine Tataru, Maya Livni, Carrie Marean-Reardon, Maria Clara Franco, Maude David and Masanori A. Murayama
PLoS ONE, 2023, 18, e0289314
DOI: 10.1371/journal.pone.0289314

Mesenchymal Stem Cell and Hematopoietic Stem and Progenitor Cell Co-Culture in a Bone-Marrow-on-a-Chip Device toward the Generation and Maintenance of the Hematopoietic Niche
Dionysia Kefallinou, Maria Grigoriou, Dimitrios T. Boumpas and Angeliki Tserepi
Bioengineering, 2024, 11, 748
DOI: 10.3390/bioengineering11080748

Investigation of omeprazole and phenacetin first‐pass metabolism in humans using a microscale bioreactor and pharmacokinetic models
Thibault Bricks, Jérémy Hamon, Marie José Fleury, Rachid Jellali, Franck Merlier, Yves Edouard Herpe, Alexandre Seyer, Jean‐Marc Regimbeau, Frédéric Bois and Eric Leclerc
Biopharm & Drug Disp, 2015, 36, 275
DOI: 10.1002/bdd.1940

Microfluidic organs-on-chips
Sangeeta N Bhatia and Donald E Ingber
Nat Biotechnol, 2014, 32, 760
DOI: 10.1038/nbt.2989

Gut microbiota in toxicological risk assessment of drugs and chemicals: The need of hour
Ganesan Velmurugan
Gut Microbes, 2018, 1
DOI: 10.1080/19490976.2018.1445955

Fluoropolymer Functionalization of Organ-on-Chip Platform Increases Detection Sensitivity for Cannabinoids
Ziqiu Tong, Lars Esser, Peter Galettis, David Rudd, Christopher D. Easton, Azadeh Nilghaz, Bo Peng, Douer Zhu, Helmut Thissen, Jennifer H. Martin and Nicolas H. Voelcker
Biosensors, 2023, 13, 779
DOI: 10.3390/bios13080779

Microfluidic Gut-on-a-Chip: Fundamentals and Challenges
Dimple Palanilkunnathil Thomas, Jun Zhang, Nam-Trung Nguyen and Hang Thu Ta
Biosensors, 2023, 13, 136
DOI: 10.3390/bios13010136

How to Determine the Role of the Microbiome in Drug Disposition
Jordan E. Bisanz, Peter Spanogiannopoulos, Lindsey M. Pieper, Annamarie E. Bustion and Peter J. Turnbaugh
Drug Metabolism and Disposition, 2018, 46, 1588
DOI: 10.1124/dmd.118.083402

Evolution of a confluent gut epithelium under on-chip cyclic stretching
Lauriane Gérémie, Efe Ilker, Moencopi Bernheim-Dennery, Charles Cavaniol, Jean-Louis Viovy, Danijela Matic Vignjevic, Jean-François Joanny and Stéphanie Descroix
Phys. Rev. Research, 2022, 4
DOI: 10.1103/PhysRevResearch.4.023032

Intestinal Stem Cells to Advance Drug Development, Precision, and Regenerative Medicine: A Paradigm Shift in Translational Research
Jonathan P. Mochel, Albert E. Jergens, Dawn Kingsbury, Hyun Jung Kim, Martín G. Martín and Karin Allenspach
AAPS J, 2018, 20
DOI: 10.1208/s12248-017-0178-1

Human enteroids as a tool to study conventional and ultra-high dose rate radiation
Katarina C Klett, Briana C Martin-Villa, Victoria S Villarreal, Stavros Melemenidis, Vignesh Viswanathan, Rakesh Manjappa, M Ramish Ashraf, Luis Soto, Brianna Lau, Suparna Dutt, Erinn B Rankin, Billy W Loo and Sarah C Heilshorn
Integrative Biology, 2023, 15
DOI: 10.1093/intbio/zyad013

A Biomimetic Human Gut‐on‐a‐Chip for Modeling Drug Metabolism in Intestine
Yaqiong Guo, Zhongyu Li, Wentao Su, Li Wang, Yujuan Zhu and Jianhua Qin
Artificial Organs, 2018, 42, 1196
DOI: 10.1111/aor.13163

Maintenance of Head and Neck Tumor On-Chip: Gateway to Personalized Treatment?
Ruth Bower, Victoria L Green, Elena Kuvshinova, Dmitriy Kuvshinov, Laszlo Karsai, Stephen T Crank, Nicholas D Stafford and John Greenman
Future Sci. OA, 2017, 3
DOI: 10.4155/fsoa-2016-0089

The Microbiome and the Gut‐Liver‐Brain Axis for Central Nervous System Clinical Pharmacology: Challenges in Specifying and Integrating In Vitro and In Silico Models
Kyle G. Hawkins, Caleb Casolaro, Jacquelyn A. Brown, David A. Edwards and John P. Wikswo
Clin Pharma and Therapeutics, 2020, 108, 929
DOI: 10.1002/cpt.1870

Modeling Human Nutrition Using Human Embryonic Stem Cells
Danny Ben-Zvi and Douglas A. Melton
Cell, 2015, 161, 12
DOI: 10.1016/j.cell.2015.02.039

Cell Systems to Investigate the Impact of Polyphenols on Cardiovascular Health
Charlotte Grootaert, Senem Kamiloglu, Esra Capanoglu and John Van Camp
Nutrients, 2015, 7, 9229
DOI: 10.3390/nu7115462

A comprehensive overview of advanced dynamic in vitro intestinal and hepatic cell culture models
Filipa Leal, Scarlett Zeiringer, Ramona Jeitler, Pedro F. Costa and Eva Roblegg
Tissue Barriers, 2024, 12
DOI: 10.1080/21688370.2022.2163820

Biomorphogenesis-Inspired Three-Dimensional Shape Transformation of Bilayer Polymer Sheets
Kai Gou, Lei Wang, Minghui Wei, Jingtao Jiang and Gengsheng Weng
Chem. Mater., 2024, 36, 3754
DOI: 10.1021/acs.chemmater.4c00049

An In Vitro Model for the Ocular Surface and Tear Film System
Qiaozhi Lu, Hongbo Yin, Michael P. Grant and Jennifer H. Elisseeff
Sci Rep, 2017, 7
DOI: 10.1038/s41598-017-06369-8

Gastrointestinal microphysiological systems
Sarah E Blutt, James R Broughman, Winnie Zou, Xi-Lei Zeng, Umesh C Karandikar, Julie In, Nicholas C Zachos, Olga Kovbasnjuk, Mark Donowitz and Mary K Estes
Exp Biol Med (Maywood), 2017, 242, 1633
DOI: 10.1177/1535370217710638

A flexible strategy to fabricate trumpet-shaped porous PDMS membranes for organ-on-chip application
Yingying Xie, Yaqiong Guo, Fuwei Xie, Yan Dong, Xiaoqing Zhang, Xiang Li and Xu Zhang
Biomicrofluidics, 2024, 18
DOI: 10.1063/5.0227148

Physiologically Based Pharmacokinetic and Pharmacodynamic Analysis Enabled by Microfluidically Linked Organs-on-Chips
Rachelle Prantil-Baun, Richard Novak, Debarun Das, Mahadevabharath R. Somayaji, Andrzej Przekwas and Donald E. Ingber
Annu. Rev. Pharmacol. Toxicol., 2018, 58, 37
DOI: 10.1146/annurev-pharmtox-010716-104748

Biomechanical design principles of intestine-on-chip models: past, present and future directions
Rafsan Ahmed Rashik, Olivia Guanella, Wenbin Mao and Nathan D Gallant
Biomed. Mater., 2026, 21, 022006
DOI: 10.1088/1748-605X/ae5437

A systematic investigation of the effect of the fluid shear stress on Caco-2 cells towards the optimization of epithelial organ-on-chip models
Ludivine C. Delon, Zhaobin Guo, Anna Oszmiana, Chia-Chi Chien, Rachel Gibson, Clive Prestidge and Benjamin Thierry
Biomaterials, 2019, 225, 119521
DOI: 10.1016/j.biomaterials.2019.119521

Human Intestinal Morphogenesis Controlled by Transepithelial Morphogen Gradient and Flow-Dependent Physical Cues in a Microengineered Gut-on-a-Chip
Woojung Shin, Christopher D. Hinojosa, Donald E. Ingber and Hyun Jung Kim
iScience, 2019, 15, 391
DOI: 10.1016/j.isci.2019.04.037

The Impact of the Host Intestinal Microbiome on Carcinogenesis and the Response to Chemotherapy
Jaime N Wertman, Katherine A Dunn and Ketan Kulkarni
Future Oncol., 2021, 17, 4371
DOI: 10.2217/fon-2021-0087

Organotypic and Microphysiological Human Tissue Models for Drug Discovery and Development—Current State-of-the-Art and Future Perspectives
Sonia Youhanna, Aurino M. Kemas, Lena Preiss, Yitian Zhou, Joanne X. Shen, Selgin D. Cakal, Francesco S. Paqualini, Sravan K. Goparaju, Reza Zandi Shafagh, Johan Ulrik Lind, Carl M. Sellgren and Volker M. Lauschke
Pharmacological Reviews, 2022, 74, 141
DOI: 10.1124/pharmrev.120.000238

Ex vivo and miniaturized in vitro models to study microbiota-gut-brain axis
Vinod Kumar Yata
3 Biotech, 2024, 14
DOI: 10.1007/s13205-024-04126-z

A microengineered collagen scaffold for generating a polarized crypt-villus architecture of human small intestinal epithelium
Yuli Wang, Dulan B. Gunasekara, Mark I. Reed, Matthew DiSalvo, Scott J. Bultman, Christopher E. Sims, Scott T. Magness and Nancy L. Allbritton
Biomaterials, 2017, 128, 44
DOI: 10.1016/j.biomaterials.2017.03.005

The Human Microbiome and Obesity: Moving beyond Associations
Padma Maruvada, Vanessa Leone, Lee M. Kaplan and Eugene B. Chang
Cell Host & Microbe, 2017, 22, 589
DOI: 10.1016/j.chom.2017.10.005

Development of Microplatforms to Mimic the In Vivo Architecture of CNS and PNS Physiology and Their Diseases
John Saliba, Arij Daou, Samar Damiati, Jessica Saliba, Marwan El-Sabban and Rami Mhanna
Genes, 2018, 9, 285
DOI: 10.3390/genes9060285

Cells in the 3D biomatrix on-chip: better mimicking the real micro-physiological system
Michele D’Orazio, Joanna Filippi, Gianni Antonelli, Giorgia Curci, Paola Casti, Arianna Mencattini, Gianluca Cidonio and Eugenio Martinelli
Next Materials, 2024, 5, 100229
DOI: 10.1016/j.nxmate.2024.100229

Intestine‐on‐chip device increases ECM remodeling inducing faster epithelial cell differentiation
Vincenza De Gregorio, Brunella Corrado, Simone Sbrescia, Sara Sibilio, Francesco Urciuolo, Paolo A. Netti and Giorgia Imparato
Biotech & Bioengineering, 2020, 117, 556
DOI: 10.1002/bit.27186

Microfluidic Device Using Mouse Small Intestinal Tissue for the Observation of Fluidic Behavior in the Lumen
Satoru Kuriu, Naoyuki Yamamoto and Tadashi Ishida
Micromachines, 2021, 12, 692
DOI: 10.3390/mi12060692

Organoids: Modeling Development and the Stem Cell Niche in a Dish
Kai Kretzschmar and Hans Clevers
Developmental Cell, 2016, 38, 590
DOI: 10.1016/j.devcel.2016.08.014

Progress and future of in vitro models to study translocation of nanoparticles
Hedwig M. Braakhuis, Samantha K. Kloet, Sanja Kezic, Frieke Kuper, Margriet V. D. Z. Park, Susann Bellmann, Meike van der Zande, Séverine Le Gac, Petra Krystek, Ruud J. B. Peters, Ivonne M. C. M. Rietjens and Hans Bouwmeester
Arch Toxicol, 2015, 89, 1469
DOI: 10.1007/s00204-015-1518-5

Priming nanoparticle-guided diagnostics and therapeutics towards human organs-on-chips microphysiological system
Jin-Ha Choi, Jaewon Lee, Woojung Shin, Jeong-Woo Choi and Hyun Jung Kim
Nano Convergence, 2016, 3
DOI: 10.1186/s40580-016-0084-8

Lost in Translation: The Need for Better Tools
Susanna Penco, Elena Venco and Alfredo Lio
Altern Lab Anim, 2014, 42, P41
DOI: 10.1177/026119291404200411

A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures
Martin Dufva
Sci Rep, 2023, 13
DOI: 10.1038/s41598-023-35043-5

Intestinal immune responses driven by gut microbial metabolite-GPCR signaling and advances in human in vitro models
Eri OGURO-IGASHIRA and Kiyoshi TAKEDA
Translational and Regulatory Sciences, 2025, 7, 94
DOI: 10.33611/trs.2025-014

Modelling host–microbiome interactions in organ-on-a-chip platforms
Yong Cheol Shin, Nam Than, Soyoun Min, Woojung Shin and Hyun Jung Kim
Nat Rev Bioeng, 2023, 2, 175
DOI: 10.1038/s44222-023-00130-9

Medium throughput breathing human primary cell alveolus-on-chip model
Janick D. Stucki, Nina Hobi, Artur Galimov, Andreas O. Stucki, Nicole Schneider-Daum, Claus-Michael Lehr, Hanno Huwer, Manfred Frick, Manuela Funke-Chambour, Thomas Geiser and Olivier T. Guenat
Sci Rep, 2018, 8
DOI: 10.1038/s41598-018-32523-x

Flow-induced mechano-modulation of intestinal permeability on chip
Raffaele Mennella, Sara Sibilio, Francesco Urciuolo, Giorgia Imparato and Paolo A. Netti
Materials Today Bio, 2025, 33, 101951
DOI: 10.1016/j.mtbio.2025.101951

Hydrogel-Integrated Millifluidic Systems: Advancing the Fabrication of Mucus-Producing Human Intestinal Models
Ahed Almalla, Nadra Alzain, Laura Elomaa, Fiona Richter, Johanna Scholz, Marcus Lindner, Britta Siegmund and Marie Weinhart
Cells, 2024, 13, 1080
DOI: 10.3390/cells13131080

Unravelling animal–microbiota evolution on a chip
Ostaizka Aizpurua, Kees Blijleven, Urvish Trivedi, M. Thomas P. Gilbert and Antton Alberdi
Trends in Microbiology, 2023, 31, 995
DOI: 10.1016/j.tim.2023.04.010

Technologies for Measuring Pharmacokinetic Profiles
A.A. Heller, S.Y. Lockwood, T.M. Janes and D.M. Spence
Annual Rev. Anal. Chem., 2018, 11, 79
DOI: 10.1146/annurev-anchem-061417-125611

Farewell to Animal Testing: Innovations on Human Intestinal Microphysiological Systems
Tae Kang and Hyun Kim
Micromachines, 2016, 7, 107
DOI: 10.3390/mi7070107

Development and validation of bioengineered intestinal tubules for translational research aimed at safety and efficacy testing of drugs and nutrients
Paulus G.M. Jochems, Jeroen van Bergenhenegouwen, Anne Metje van Genderen, Sophie T. Eis, Livia J.F. Wilod Versprille, Harry J. Wichers, Prescilla V. Jeurink, Johan Garssen and Rosalinde Masereeuw
Toxicology in Vitro, 2019, 60, 1
DOI: 10.1016/j.tiv.2019.04.019

Ready-to-Use Nanofiber Membrane-Integrated Organ-on-a-Chip via Cryopreservation of Cell-Loaded Microfluidic Chip
Dohui Kim, Soojin Yi, Byeong-ung Park, Seong Jin Lee, Jeongmin Lee, Dong Sung Kim and Hong Kyun Kim
ACS Macro Lett., 2025, 14, 622
DOI: 10.1021/acsmacrolett.5c00129

Comparative study of the transcriptomes of Caco-2 cells cultured under dynamic vs. static conditions following exposure to titanium dioxide and zinc oxide nanomaterials
Kornphimol Kulthong, Guido J. E. J. Hooiveld, Loes P. M. Duivenvoorde, Ignacio Miro Estruch, Hans Bouwmeester and Meike van der Zande
Nanotoxicology, 2021, 15, 1233
DOI: 10.1080/17435390.2021.2012609

Deciphering respiratory viral infections by harnessing organ-on-chip technology to explore the gut–lung axis
Hristina Koceva, Mona Amiratashani, Parastoo Akbarimoghaddam, Bianca Hoffmann, Gaukhar Zhurgenbayeva, Mark S. Gresnigt, Vanessa Rossetto Marcelino, Christian Eggeling, Marc Thilo Figge, Maria-João Amorim and Alexander S. Mosig
Open Biol., 2025, 15
DOI: 10.1098/rsob.240231

Organs-on-chips: into the next decade
Lucie A. Low, Christine Mummery, Brian R. Berridge, Christopher P. Austin and Danilo A. Tagle
Nat Rev Drug Discov, 2021, 20, 345
DOI: 10.1038/s41573-020-0079-3

Nanoliposomes Permeability in a Microfluidic Drug Delivery Platform across a 3D Hydrogel
Corentin Peyret, Aleka Manousaki, Sabine Bouguet-Bonnet, Emmanuel Stratakis, Laura Sanchez-Gonzalez, Cyril J.F. Kahn and Elmira Arab-Tehrany
Pharmaceutics, 2024, 16, 765
DOI: 10.3390/pharmaceutics16060765

Revolutionizing the female reproductive system research using microfluidic chip platform
Jinfeng Yan, Tong Wu, Jinjin Zhang, Yueyue Gao, Jia-Min Wu and Shixuan Wang
J Nanobiotechnol, 2023, 21
DOI: 10.1186/s12951-023-02258-7

A Comprehensive Review of Nanoparticles for Oral Delivery in Food: Biological Fate, Evaluation Models, and Gut Microbiota Influences
Jingyi Xue, Christopher Blesso and Yangchao Luo
Annu. Rev. Food Sci. Technol., 2023, 14, 1
DOI: 10.1146/annurev-food-060721-025159

Advancements and Challenges in Modeling Mechanobiology in Intestinal Host-Microbiota Interaction
Hong Sheng Cheng, Yee Han Tey, Si Yuan Hu, Alethea Yen Ning Yeo, Zong Heng Ngo, Joseph Han Sol Kim and Nguan Soon Tan
ACS Appl. Mater. Interfaces, 2025, 17, 31698
DOI: 10.1021/acsami.4c20961