Issue 57, 2023

Quantitative determination of the full switching cycle of photochromic fluorescent proteins

Abstract

In this study, we develop a general analytical model of the photochromism of fluorescent proteins and apply it to spectroscopic measurements performed on six different labels. Our approach provides quantitative explanations for phenomena such as the existence of positive and negative switching, limitations in the photochromism contrast, and the fact that initial switching cycles may differ from subsequent ones. It also allows us to perform the very first measurement of all four isomerization quantum yields involved in the switching process.

Graphical abstract: Quantitative determination of the full switching cycle of photochromic fluorescent proteins

Supplementary files

Article information

Article type
Communication
Submitted
02 ⵉⴱⵔ 2023
Accepted
17 ⵎⴰⵢ 2023
First published
28 ⵢⵓⵏ 2023

Chem. Commun., 2023,59, 8810-8813

Quantitative determination of the full switching cycle of photochromic fluorescent proteins

A. C. Bourges, B. Moeyaert, T. Y. H. Bui, F. Bierbuesse, W. Vandenberg and P. Dedecker, Chem. Commun., 2023, 59, 8810 DOI: 10.1039/D3CC01617J

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