A lanthanide tag for a complementary set of pseudocontact shifts

Lydia Topping; Adarshi P. Welegedara; Martyna Judd; Elwy H. Abdelkader; Nicholas Cox; Gottfried Otting; Stephen J. Butler

doi:10.1039/D4CC03007A

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A lanthanide tag for a complementary set of pseudocontact shifts†

Lydia Topping,^a Adarshi P. Welegedara,^b Martyna Judd,^c Elwy H. Abdelkader,

^b Nicholas Cox,

^c Gottfried Otting

^b and Stephen J. Butler

*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, Loughborough University, Epinal Way, Loughborough, UK
E-mail: s.j.butler@lboro.ac.uk

^b ARC Centre of Excellence for Innovations in Peptide & Protein Science, Research School of Chemistry, Australian National University, Canberra, Australia

^c Research School of Chemistry, Australian National University, Canberra, Australia

Abstract

Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.

This article is part of the themed collection: 2024 Pioneering Investigators

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Article information

DOI: https://doi.org/10.1039/D4CC03007A
Article type: Communication
Submitted: 20 ⵢⵓⵏ 2024
Accepted: 16 ⵢⵓⵍ 2024
First published: 16 ⵢⵓⵍ 2024
This article is Open Access

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Chem. Commun., 2024,60, 8458-8461

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A lanthanide tag for a complementary set of pseudocontact shifts

L. Topping, A. P. Welegedara, M. Judd, E. H. Abdelkader, N. Cox, G. Otting and S. J. Butler, Chem. Commun., 2024, 60, 8458 DOI: 10.1039/D4CC03007A

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Chemical Communications

A lanthanide tag for a complementary set of pseudocontact shifts†

Abstract

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A lanthanide tag for a complementary set of pseudocontact shifts

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