Issue 23, 2022

Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification

Abstract

We described a new system termed droplet DNAzyme-coupled rolling circle amplification (dDRCA) that can selectively detect bacteria from clinical urine samples with single-cell sensitivity within 1.5 h compared with the several hours needed for traditionally used culture-based methods.

Graphical abstract: Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification

Supplementary files

Article information

Article type
Communication
Submitted
20 ⵉⴱⵔ 2022
Accepted
11 ⵎⴰⵢ 2022
First published
12 ⵎⴰⵢ 2022

Anal. Methods, 2022,14, 2244-2248

Single bacteria detection by droplet DNAzyme-coupled rolling circle amplification

Y. Chi, M. Shi, Y. Wu, Y. Wu, Y. Chang and M. Liu, Anal. Methods, 2022, 14, 2244 DOI: 10.1039/D2AY00656A

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