Issue 20, 2022

Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

Abstract

The rapid and sensitive detection of pathogenic bacteria is highly demanded for early warning of infectious disease epidemics and protection of human health. Herein, a reusable and universal impedimetric sensing platform based on a bacteria-imprinted polythiophene film (BIF) is proposed for the rapid and sensitive detection of pathogenic bacteria using Staphylococcus aureus (S. aureus) as a model analyte. Monomer screening among four 3-substituted thiophenes was first performed based on the imprinting factor, and 3-thiopheneethanol (TE) was eventually selected. The BIF as a recognition layer was quickly deposited in an environmentally friendly process on a glassy carbon electrode via electro-copolymerization of the S. aureus template and TE monomer followed by in situ template removal. Upon rebinding of S. aureus on the BIF, the impedance increased. Under optimal conditions, the BIF-based sensor can quantitatively detect S. aureus in a wide linear range of 10 to 107 CFU mL−1 with a low detection limit of 4 CFU mL−1. Additionally, the sensor exhibits excellent selectivity, capable of identifying S. aureus from multi-bacterial strain mixtures. It also demonstrates applicability in the analysis of real lettuce and shrimp samples with good recoveries. Most significantly, the BIF sensing interface can be reused up to five times with good signal retention. Compared with most reported methods, this sensor is more rapid with a much shorter total assay time of 30 min, including the BIF preparation, bacterial rebinding, and impedance detection. This assay may hold great potential to help in the rapid, sensitive, and label-free detection of pathogenic bacteria in fields of food safety and public health.

Graphical abstract: Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

Supplementary files

Article information

Article type
Paper
Submitted
12 ⵢⵓⵍ 2022
Accepted
12 ⵖⵓⵛ 2022
First published
12 ⵖⵓⵛ 2022

Analyst, 2022,147, 4433-4441

Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film

L. Wang, X. Lin, T. Liu, Z. Zhang, J. Kong, H. Yu, J. Yan, D. Luan, Y. Zhao and X. Bian, Analyst, 2022, 147, 4433 DOI: 10.1039/D2AN01122K

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