Issue 39, 2021

Computational investigations of selected enzymes from two iron and α-ketoglutarate-dependent families

Abstract

DNA alkylation is used as the key epigenetic mark in eukaryotes, however, most alkylation in DNA can result in deleterious effects. Therefore, this process needs to be tightly regulated. The enzymes of the AlkB and Ten-Eleven Translocation (TET) families are members of the Fe and alpha-ketoglutarate-dependent superfamily of enzymes that are tasked with dealkylating DNA and RNA in cells. Members of these families span all species and are an integral part of transcriptional regulation. While both families catalyze oxidative dealkylation of various bases, each has specific preference for alkylated base type as well as distinct catalytic mechanisms. This perspective aims to provide an overview of computational work carried out to investigate several members of these enzyme families including AlkB, ALKB Homolog 2, ALKB Homolog 3 and Ten-Eleven Translocate 2. Insights into structural details, mutagenesis studies, reaction path analysis, electronic structure features in the active site, and substrate preferences are presented and discussed.

Graphical abstract: Computational investigations of selected enzymes from two iron and α-ketoglutarate-dependent families

Supplementary files

Article information

Article type
Perspective
Submitted
18 ⵖⵓⵛ 2021
Accepted
24 ⵛⵓⵜ 2021
First published
24 ⵛⵓⵜ 2021

Phys. Chem. Chem. Phys., 2021,23, 22227-22240

Author version available

Computational investigations of selected enzymes from two iron and α-ketoglutarate-dependent families

M. B. Berger, A. R. Walker, E. A. Vázquez-Montelongo and G. A. Cisneros, Phys. Chem. Chem. Phys., 2021, 23, 22227 DOI: 10.1039/D1CP03800A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements