Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody

Abstract

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed based on a sensitive and specific monoclonal antibody (mAb) against natamycin (Nata) for Nata detection in milk, juice, yoghurt, and cheese samples. The working range of ic-ELISA was 0.64–4.46 μg L⁻¹ with an IC₅₀ value of 1.69 μg L⁻¹. The average recoveries of milk, juice, yoghurt, and cheese samples spiked with Nata were 103–121%, 103–121%, 84–114%, and 89–108%, respectively. The results indicated that ic-ELISA can be effectively applied for Nata analysis in these food products.