Issue 8, 2015

Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody

Abstract

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed based on a sensitive and specific monoclonal antibody (mAb) against natamycin (Nata) for Nata detection in milk, juice, yoghurt, and cheese samples. The working range of ic-ELISA was 0.64–4.46 μg L−1 with an IC50 value of 1.69 μg L−1. The average recoveries of milk, juice, yoghurt, and cheese samples spiked with Nata were 103–121%, 103–121%, 84–114%, and 89–108%, respectively. The results indicated that ic-ELISA can be effectively applied for Nata analysis in these food products.

Graphical abstract: Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody

Article information

Article type
Paper
Submitted
12 ⴱⵕⴰ 2015
Accepted
19 ⵎⴰⵕ 2015
First published
19 ⵎⴰⵕ 2015

Anal. Methods, 2015,7, 3559-3565

Author version available

Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody

Y. Chen, D. Kong, L. Liu, S. Song, H. Kuang and C. Xu, Anal. Methods, 2015, 7, 3559 DOI: 10.1039/C5AY00404G

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