Issue 23, 2022

A microbial quantity monitoring model based on 3D fluorescence data of the cucumber storeroom gas and its use in providing auxiliary early spoilage warning

Abstract

A real-time model for monitoring the microbial quantity based on the microbial intrinsic fluorescence information of cucumber storeroom gas was established. Firstly, 3D fluorescence data of the storeroom gas were collected on different storage days. Secondly, the number of components of a parallel factor model was determined to be 3 using the core consistency diagnostic. Thirdly, parallel factor analysis was used to decompose the fluorescence data to obtain the excitation spectra, emission spectra and concentration scores of 3 components. The positions of the fluorescence peaks were consistent with the fingerprints of tryptophan-like, tyrosine-like and phenylalanine-like substances in the characteristic spectrum of each component. And then the prediction model was constructed by fitting the concentration scores of the 3 components with the microbial quantity, and the coefficient of determination was 98.27%, and the cross-validation determination coefficient could reach 91.97%. Finally, after integrating the predicted value of the microbial quantity and the total chromatism of the cucumber pericarp during cucumber storage, the spoilage date was determined to be the 7th day by K-means clustering. The results show that the monitoring model constructed through distinguishing the fluorescence data of airborne microorganisms can effectively monitor the spoilage process.

Graphical abstract: A microbial quantity monitoring model based on 3D fluorescence data of the cucumber storeroom gas and its use in providing auxiliary early spoilage warning

Article information

Article type
Paper
Submitted
12 ⵢⵓⵍ 2022
Accepted
08 ⴽⵜⵓ 2022
First published
11 ⴽⵜⵓ 2022

Analyst, 2022,147, 5347-5354

A microbial quantity monitoring model based on 3D fluorescence data of the cucumber storeroom gas and its use in providing auxiliary early spoilage warning

Y. Yuan, X. Liu, Y. Yin, H. Yu, J. Chen and M. Li, Analyst, 2022, 147, 5347 DOI: 10.1039/D2AN01121B

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