Issue 6, 2016

Segmented flow sampling with push–pull theta pipettes

Abstract

We report development of a mobile and easy-to-fabricate theta pipette microfluidic device for segmented flow sampling. The theta pipettes were also used as electrospray emitters for analysis of sub-nanoliter segments, which resulted in delivery of analyte to the vacuum inlet of the mass spectrometer without multiple transfer steps. Theta pipette probes enable sample collection with high spatial resolution due to micron or smaller sized probe inlets and can be used to manipulate aqueous segments in the range of 200 pL to tens of nanoliters. Optimized conditions can enable sampling with high spatial and temporal resolution, suitable for chemical monitoring in biological samples and studies of sample heterogeneity. Intercellular heterogeneity among Allium cepa cells was studied by collecting cytoplasm from multiple cells using a single probe. Extracted cytoplasm was analyzed in a fast and high throughput manner by direct electrospray mass spectrometry of segmented sample from the probe tip.

Graphical abstract: Segmented flow sampling with push–pull theta pipettes

Supplementary files

Article information

Article type
Paper
Submitted
05 ⵉⵏⵏ 2016
Accepted
17 ⴱⵕⴰ 2016
First published
17 ⴱⵕⴰ 2016

Analyst, 2016,141, 1958-1965

Segmented flow sampling with push–pull theta pipettes

A. Saha-Shah, C. M. Green, D. H. Abraham and L. A. Baker, Analyst, 2016, 141, 1958 DOI: 10.1039/C6AN00028B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements