Issue 13, 2014

Geometric effect of the hydrogel grid structure on in vitro formation of homogeneous MIN6 cell clusters

Abstract

A microstructure-based hydrogel was employed to study the relationship between spatial specificity and cellular behavior, including cell fate, proliferation, morphology, and insulin secretion in pancreatic β-cells. To effectively form homogeneous cell clusters in vitro, we made cell-containing hydrogel membrane constructs with an adapted grid structure based on a hexagonal micropattern. Homogeneous cell clusters (average diameter: 83.6 ± 14.2 μm) of pancreatic insulinoma (MIN6) cells were spontaneously generated in the floating hydrogel membrane constructs, including a hexagonal grid structure (size of cavity: 100 μm, interval between cavities: 30 μm). Interestingly, 3D clustering of MIN6 cells mimicking the structure of pancreatic islets was coalesced into a merged aggregate attaching to each hexagonal cavity of the hydrogel grid structure. The fate and insulin secretion of homogeneous cell clusters in the hydrogel grid structure were also assessed. The results of these designable hydrogel–cell membrane constructs suggest that facultative in vitro β-cell proliferation and maintenance can be applied to biofunctional assessments.

Graphical abstract: Geometric effect of the hydrogel grid structure on in vitro formation of homogeneous MIN6 cell clusters

Article information

Article type
Paper
Submitted
20 ⴷⵓⵊ 2013
Accepted
27 ⵉⵏⵏ 2014
First published
28 ⵉⵏⵏ 2014

Lab Chip, 2014,14, 2183-2190

Author version available

Geometric effect of the hydrogel grid structure on in vitro formation of homogeneous MIN6 cell clusters

C. Y. Bae, M. Min, H. Kim and J. Park, Lab Chip, 2014, 14, 2183 DOI: 10.1039/C3LC51421H

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