A dive into the bath: embedded 3D bioprinting of freeform in vitro models

M. Özgen Öztürk-Öncel, Baltazar Hiram Leal-Martínez, Rosa F. Monteiro, Manuela E. Gomes and Rui M. A. Domingues

Biomater. Sci., 2023, 11, 5462
DOI: 10.1039/D3BM00626C

Embedded bioprinted multicellular spheroids modeling pancreatic cancer bioarchitecture towards advanced drug therapy

Xiaoyun Wei, Yiwen Wu, Keke Chen, Ling Wang and Mingen Xu

J. Mater. Chem. B, 2024, 12, 1788
DOI: 10.1039/D3TB02913A

Bioactive hydrogels based on polysaccharides and peptides for soft tissue wound management

Lihuang Wu, Yiyan He, Hongli Mao and Zhongwei Gu

J. Mater. Chem. B, 2022, 10, 7148
DOI: 10.1039/D2TB00591C

Photoclick polysaccharide-based bioinks with an extended biofabrication window for 3D embedded bioprinting

Ke Zhou, Miao Feng, Hongli Mao and Zhongwei Gu

Biomater. Sci., 2022, 10, 4479
DOI: 10.1039/D2BM00632D

Embedded 3D Bioprinting of Collagen Inks into Microgel Baths to Control Hydrogel Microstructure and Cell Spreading

Lucia G. Brunel, Fotis Christakopoulos, David Kilian, Betty Cai, Sarah M. Hull, David Myung and Sarah C. Heilshorn

Adv Healthcare Materials, 2024
DOI: 10.1002/adhm.202303325

Hydrogels in Gene Delivery Techniques for Regenerative Medicine and Tissue Engineering

Kexing Xu, Qinmeng Zhang, Danji Zhu and Zhiwei Jiang

Macromolecular Bioscience, 2024, 24
DOI: 10.1002/mabi.202300577

Biofabrication & cryopreservation of tissue engineered constructs for on-demand applications

Harshavardhan Budharaju, Dhakshinamoorthy Sundaramurthi and Swaminathan Sethuraman

Biofabrication, 2024, 16, 042008
DOI: 10.1088/1758-5090/ad7906

Suspended bioprinting with in-situ elasticity monitoring using the assessment of shear wave phase velocity

Garin Kim, Dageon Oh, Dasong Kim, Ganghak Lee, Sang-Hyug Park, Changhan Yoon and Seung Yun Nam

Additive Manufacturing, 2025, 99, 104667
DOI: 10.1016/j.addma.2025.104667

A Guide to Polysaccharide-Based Hydrogel Bioinks for 3D Bioprinting Applications

Maria C. Teixeira, Nicole S. Lameirinhas, João P. F. Carvalho, Armando J. D. Silvestre, Carla Vilela and Carmen S. R. Freire

IJMS, 2022, 23, 6564
DOI: 10.3390/ijms23126564

A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications

Mecit Altan Alioglu, Yasar Ozer Yilmaz, Ethan Michael Gerhard, Vaibhav Pal, Deepak Gupta, Syed Hasan Askari Rizvi and Ibrahim T. Ozbolat

Adv Materials Technologies, 2024, 9
DOI: 10.1002/admt.202301858

Cation-crosslinked κ-carrageenan sub-microgel medium for high-quality embedded bioprinting

Hua Zhang, Yang Luo, Zeming Hu, Mengxi Chen, Shang Chen, Yudong Yao, Jie Yao, Xiaoqi Shao, Kerong Wu, Yabin Zhu and Jun Fu

Biofabrication, 2024, 16, 025009
DOI: 10.1088/1758-5090/ad1cf3

Dissecting the Interplay Mechanism among Process Parameters toward the Biofabrication of High‐Quality Shapes in Embedded Bioprinting

Yang Wu, Xue Yang, Deepak Gupta, Mecit Altan Alioglu, Minghao Qin, Veli Ozbolat, Yao Li and Ibrahim T. Ozbolat

Adv Funct Materials, 2024, 34
DOI: 10.1002/adfm.202313088

3D Bioprinting of Liquid High‐Cell‐Proportion Bioinks in Liquid Granular Bath

Jinhong Jiang, Chenhui Yuan, Xinyu Zhang, Lin Gu, Yudong Yao, Xueping Wang, Yong He and Lei Shao

Advanced Materials, 2024, 36
DOI: 10.1002/adma.202412127

Embedded extrusion printing in yield-stress-fluid baths

Qian Wu, Kaidong Song, Deming Zhang, Bing Ren, Marc Sole-Gras, Yong Huang and Jun Yin

Matter, 2022, 5, 3775
DOI: 10.1016/j.matt.2022.09.003

The use of fluid-phase 3D printing to pattern alginate-gelatin hydrogel properties to guide cell growth and behaviour in vitro

Andrea Souza, McCarthy Kevin, Brian J Rodriguez and Emmanuel G Reynaud

Biomed. Mater., 2024, 19, 045024
DOI: 10.1088/1748-605X/ad51bf

FRESH Bioprinting of Dynamic Hydrazone-Cross-Linked Synthetic Hydrogels

Eva Mueller, Fei Xu and Todd Hoare

Biomacromolecules, 2022, 23, 4883
DOI: 10.1021/acs.biomac.2c01046

Recent progress of Bioinspired Hydrogel-based delivery system for endometrial repair

Rong Dong, Saihua Ma, Xiaoli Zhao, Baojuan Wang, Mridul Roy, Lu Yao, Tian Xia and Yanting Liu

Front. Bioeng. Biotechnol., 2022, 10
DOI: 10.3389/fbioe.2022.1013217

3D printing of an artificial intelligence-generated patient-specific coronary artery segmentation in a support bath

Serkan Sokmen, Soner Cakmak and Ilkay Oksuz

Biomed. Mater., 2024, 19, 035038
DOI: 10.1088/1748-605X/ad3f60

Gel-Based Suspension Medium Used in 3D Bioprinting for Constructing Tissue/Organ Analogs

Yang Luo, Rong Xu, Zeming Hu, Renhao Ni, Tong Zhu, Hua Zhang and Yabin Zhu

Gels, 2024, 10, 644
DOI: 10.3390/gels10100644

Recent Advances in the Application of 3D-Printing Bioinks Based on Decellularized Extracellular Matrix in Tissue Engineering

Haoxin Wan, Jian Xiang, Guocai Mao, Shu Pan, Bing Li and Yi Lu

ACS Omega, 2024, 9, 24219
DOI: 10.1021/acsomega.4c02847

A comprehensive review on bioink based microfluidic devices

Kajal P. Chamate, Bhuvaneshwari D. Patil, Nikita V. Bhosale, Nutan V. Desai, Prasad V. Kadam, Avirup Chakraborty and Ravindra V. Badhe

Bioprinting, 2024, 44, e00371
DOI: 10.1016/j.bprint.2024.e00371

Particle–polymer interactions for 3D printing material design

Kellen Mitchell, Weijian Hua, Erick Bandala, Akhilesh K. Gaharwar and Yifei Jin

Chemical Physics Reviews, 2024, 5
DOI: 10.1063/5.0179181

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

Francesca K. Lewns, Olga Tsigkou, Liam R. Cox, Ricky D. Wildman, Liam M. Grover and Gowsihan Poologasundarampillai

Advanced Materials, 2023, 35
DOI: 10.1002/adma.202301670

Cell viability in extrusion bioprinting: the impact of process parameters, bioink rheology, and cell mechanics

Patrick J. McCauley, Catherine A. Fromen and Alexandra V. Bayles

Rheol Acta, 2025
DOI: 10.1007/s00397-025-01504-z

Multiscale Biofabrication: Integrating Additive Manufacturing with DNA‐Programmable Self‐Assembly

Anna C. Jäkel, Michael Heymann and Friedrich C. Simmel

Advanced Biology, 2023, 7
DOI: 10.1002/adbi.202200195

A modular flow process intensification towards lipid peptoids nano assembly formation

Thaissa P. F. Rosalba, Guilherme J. P. Gonçalves, Carlos Eduardo M. Salvador, Alexandre Fonseca and Carlos Kleber Z. Andrade

J Flow Chem, 2024, 14, 677
DOI: 10.1007/s41981-024-00338-9

3D bioprinting vascular networks in suspension baths

Shuai Li, Jiale Jin, Chengran Zhang, Xue Yang, Yihao Liu, Pengfei Lei and Yihe Hu

Applied Materials Today, 2023, 30, 101729
DOI: 10.1016/j.apmt.2022.101729

PVA-based bioinks for 3D bioprinting: A comprehensive review of their applications in tissue engineering

Narges Johari, Zary Adabavazeh and Francesco Baino

Bioprinting, 2025, 49, e00419
DOI: 10.1016/j.bprint.2025.e00419

Direct Ink Writing of Conductive Hydrogels

Monica Ho, Aline Braz Ramirez, Negar Akbarnia, Eric Croiset, Elisabeth Prince, Gerald G. Fuller and Milad Kamkar

Adv Funct Materials, 2025, 35
DOI: 10.1002/adfm.202415507

3D bioprinting of tumor models and potential applications

Huaixu Li, Yang Qiao, Xingliang Dai, Haotian Tian, Zhenyu Han, Sheng Cheng, Peng Gao and Hongwei Cheng

Bio-des. Manuf., 2024, 7, 857
DOI: 10.1007/s42242-024-00317-y

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion‐Volumetric Printing of Microgels

Davide Ribezzi, Marième Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda and Riccardo Levato

Advanced Materials, 2023, 35
DOI: 10.1002/adma.202301673

Scaffold internal network bioprinting for vascularized tissue regeneration

Litao Suo, Yaqi Guo, Shan Mou, Yichao Jin, Dandan Zou, Jiaming Sun and Zhenxing Wang

Composites Part B: Engineering, 2025, 299, 112401
DOI: 10.1016/j.compositesb.2025.112401

Advancements of 3D bioprinting in regenerative medicine: Exploring cell sources for organ fabrication

Yue Ma, Bo Deng, Runbang He and Pengyu Huang

Heliyon, 2024, 10, e24593
DOI: 10.1016/j.heliyon.2024.e24593

The influence of viscosity of hydrogels on the spreading and migration of cells in 3D bioprinted skin cancer models

Lissinda H. Du Plessis, Chrisna Gouws and Daniel Nieto

Front. Cell Dev. Biol., 2024, 12
DOI: 10.3389/fcell.2024.1391259

Omnidirectional anisotropic embedded 3D bioprinting

Lei Shao, Jinhong Jiang, Chenhui Yuan, Xinyu Zhang, Lin Gu and Xueping Wang

Materials Today Bio, 2024, 27, 101160
DOI: 10.1016/j.mtbio.2024.101160

Comparative analysis of the residues of granular support bath materials on printed structures in embedded extrusion printing

Jinfeng Zeng, Nozomi Kasahara, Zhengtian Xie, Fiona Louis, Donghee Kang, Yasumasa Dekishima, Setsuka Kuwagaki, Norihito Sakai and Michiya Matsusaki

Biofabrication, 2023, 15, 035013
DOI: 10.1088/1758-5090/acce09

Biomaterials of human source for 3D printing strategies

João Rocha Maia, Rita Sobreiro-Almeida, Franck Cleymand and João F Mano

J. Phys. Mater., 2023, 6, 012002
DOI: 10.1088/2515-7639/acada1

Engineering biomaterials by inkjet printing of hydrogels with functional particulates

Cih Cheng, Eric J Williamson, George T.-C. Chiu and Bumsoo Han

Med-X, 2024, 2
DOI: 10.1007/s44258-024-00024-4

Multi‐material Volumetric Bioprinting and Plug‐and‐play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics

Davide Ribezzi, Jan‐Philip Zegwaart, Thomas Van Gansbeke, Aitor Tejo‐Otero, Sammy Florczak, Joska Aerts, Paul Delrot, Andreas Hierholzer, Martin Fussenegger, Jos Malda, Jos Olijve and Riccardo Levato

Advanced Materials, 2025, 37
DOI: 10.1002/adma.202409355

3D bioprinting approaches for spinal cord injury repair

Jingwei Jiu, Haifeng Liu, Dijun Li, Jiarong Li, Lu Liu, Wenjie Yang, Lei Yan, Songyan Li, Jing Zhang, Xiaoke Li, Jiao Jiao Li and Bin Wang

Biofabrication, 2024, 16, 032003
DOI: 10.1088/1758-5090/ad3a13

A mechanistic model for overhang limits in additive manufacturing

Yash Mittal, Vedant Agarwal, Dixita Yadav, Kossi Loic Avegnon, Michael Sealy, Pushkar Kamble, Gopal Gote, Yogesh Patil, Avinash Mehta, Paras Mandal and K. P. Karunakaran

Prog Addit Manuf, 2025
DOI: 10.1007/s40964-025-01154-w

Exploring the Potential of Artificial Intelligence for Hydrogel Development—A Short Review

Irina Negut and Bogdan Bita

Gels, 2023, 9, 845
DOI: 10.3390/gels9110845

In‐Foam Bioprinting: An Embedded Bioprinting Technique with Self‐Removable Support Bath

Elias Madadian, Hossein Ravanbakhsh, Francesco Touani Kameni, Maedeh Rahimnejad, Sophie Lerouge and Ali Ahmadi

Small Science, 2024, 4
DOI: 10.1002/smsc.202300280

Enhancing cell survival in 3D printing of organoids using innovative bioinks loaded with pre-cellularized porous microscaffolds

Adrien Rousselle, Arielle Ferrandon, Eric Mathieu, Julien Godet, Vincent Ball, Leo Comperat, Hugo Oliveira, Philippe Lavalle, Dominique Vautier and Youri Arntz

Bioprinting, 2022, 28, e00247
DOI: 10.1016/j.bprint.2022.e00247

“Out‐of‐the‐box” Granular Gel Bath Based on Cationic Polyvinyl Alcohol Microgels for Embedded Extrusion Printing

Jinfeng Zeng, Zhengtian Xie, Yasumasa Dekishima, Setsuka Kuwagaki, Norihito Sakai and Michiya Matsusaki

Macromol. Rapid Commun., 2023, 44
DOI: 10.1002/marc.202300025