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Engineering molecular switches of CRISPR/Cas12a for biosensing applications

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Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

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CRISPR/Cas-Based Diagnostics in Agricultural Applications

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Photocontrolled Programmable Enzymatic Cascade for Robust CRISPR Diagnostics

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J. Am. Chem. Soc., 2025, 147, 31004
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Enhancement and inactivation effect of CRISPR/Cas12a via extending hairpin activators for detection of transcription factors

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Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits

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Multiplex CRISPR‐Cas Assay for Rapid, Isothermal and Visual Detection of White Spot Syndrome Virus (WSSV) and Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimp

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Journal of Fish Diseases, 2025, 48
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Harnessing noncanonical trans-cleavage characteristics of Cas12 and Cas13a to enhance CRISPR-based diagnostics

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A label-free and rapid fluorometric strategy for microRNA detection using CRISPR-Cas12a coupled with copper nanoparticles

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Single-Tube Dual-Gene Detection of Methicillin-Resistant Staphylococcus aureus via Selective Trans-Cleavage Preferences of Cas9 and Cas12a

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Anal. Chem., 2026, 98, 3772
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Re-engineering detection Paradigms: Atypical reporters in CRISPR/Cas12a-based biosensing

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Integration of CRISPR/Cas12a and Toehold‐Mediated Strand Displacement for Alternative Conventional miRNA Detection

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Amplification-Free CRISPR Diagnostics for Point-of-Care Testing

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Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a

Jiongyu Zhang, Ziyue Li, Chong Guo, Xin Guan, Lori Avery, David Banach and Changchun Liu

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CRISPR-on-Chip for Point-of-Care Diagnostics

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Identification of a key nucleotide influencing Cas12a crRNA activity for universal photo-controlled CRISPR diagnostics

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Boosting CRISPR/Cas12a intrinsic RNA detection capability through pseudo hybrid DNA–RNA substrate design

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Cas12a-based primer production enables isothermal amplification for nucleic acid detection

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FRETting about CRISPR-Cas Assays: Dual-Channel Reporting Lowers Detection Limits and Times-to-Result

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ACS Sens., 2024, 9, 3616
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Technologies for Targeted RNA Degradation and Induced RNA Decay

Sigitas Mikutis and Gonçalo J. L. Bernardes

Chem. Rev., 2024, 124, 13301
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Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a system

Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen and Chien-Hung Yu

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Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing

Fei Deng, Yi Li, Biyao Yang, Rui Sang, Wei Deng, Maya Kansara, Frank Lin, Subotheni Thavaneswaran, David M. Thomas and Ewa M. Goldys

Nat Commun, 2024, 15
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From mechanism to sensitivity: Engineering CRISPR/Cas systems for clinical-grade diagnostics

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Rational Design of a Hydrophilic Perovskite Ultraviolet Photocatalyst for Mediating a Sensitive and Controllable Nucleic Acid Assay

Shuo Wang, Qi Xu, Zhiwen Gan, Yongzhen Liu, Jinxuan Hou, Long Yu and Yuxiu Xiao

ACS Nano, 2025, 19, 35468
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Towards understanding Trans-cleavage of natural and synthetic nucleic acids by Cas12a for sensitive CRISPR biosensing

Fei Deng, Rui Sang, Yi Li, Danting Yang, Flyn Mckinnirey, Wei Deng and Ewa M. Goldys

Microchemical Journal, 2024, 207, 111850
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Intrinsic RNA Targeting Triggers Indiscriminate DNase Activity of CRISPR‐Cas12a

Jiongyu Zhang, Ziyue Li, Chong Guo, Xin Guan, Lori Avery, David Banach and Changchun Liu

Angew Chem Int Ed, 2024, 63
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