Tyramide signal amplification for a highly sensitive multiplex immunoassay based on encoded hydrogel microparticles

Jun Hee Choi, Young Hee Kim, Jiwoo Kim, Yong Jun Lim, Min Jung Kim and Ki Wan Bong

Analyst, 2025, 150, 2118
DOI: 10.1039/D5AN00078E

Linker-free antibody conjugation for sensitive hydrogel microparticle-based multiplex immunoassay

Hyun Jee Lee, Ju Yeon Kim, Yoon Ho Roh, Sun Min Kim and Ki Wan Bong

Analyst, 2019, 144, 6712
DOI: 10.1039/C9AN01243E

Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors

David Rettke, Christian Danneberg, Talika Alina Neuendorf, Sebastian Kühn, Jens Friedrichs, Nicolas Hauck, Carsten Werner, Julian Thiele and Tilo Pompe

J. Mater. Chem. B, 2022, 10, 1663
DOI: 10.1039/D1TB02798K

A platform for multiplexed colorimetric microRNA detection using shape-encoded hydrogel particles

Nidhi Juthani and Patrick S. Doyle

Analyst, 2020, 145, 5134
DOI: 10.1039/D0AN00938E

Hydrogel particles-on-chip (HyPoC): a fluorescence micro-sensor array for IgG immunoassay

Alessandra De Masi, Pasqualina Liana Scognamiglio, Edmondo Battista, Paolo Antonio Netti and Filippo Causa

Lab Chip, 2023, 23, 2458
DOI: 10.1039/D2LC01080A

Precipitation-based colorimetric multiplex immunoassay in hydrogel particles

Yoon Ho Roh, Hyun Jee Lee, Ju Yeon Kim, Hyeon Ung Kim, Sun Min Kim and Ki Wan Bong

Lab Chip, 2020, 20, 2841
DOI: 10.1039/D0LC00325E

One-step assembly of barcoded planar microparticles for efficient readout of multiplexed immunoassay

Sangwook Bae, Daewon Lee, Hunjong Na, Jisung Jang and Sunghoon Kwon

Lab Chip, 2022, 22, 2090
DOI: 10.1039/D2LC00174H

Phosphorylcholine-based encoded hydrogel microparticles with enhanced fouling resistance for multiplex immunoassays

Yoon Ho Roh, Jiae Seo, Ju Yeon Kim, Hyeon Ung Kim, Seok Joon Mun, Ji-Hun Seo and Ki Wan Bong

Analyst, 2020, 145, 5482
DOI: 10.1039/D0AN00808G

Multiplexed homogeneous digital immunoassay based on single-particle motion analysis

Kenji Akama and Hiroyuki Noji

Lab Chip, 2020, 20, 2113
DOI: 10.1039/D0LC00079E

Air-through-precursor suction-augmented replica molding for fabrication of anisotropic microparticles in gas-impermeable molds

Seok Joon Mun, Wookyoung Jang, Jun Hee Choi, Yong Jun Lim and Ki Wan Bong

RSC Adv., 2024, 14, 25190
DOI: 10.1039/D4RA04719B

Degassed micromolding lithography for rapid fabrication of anisotropic hydrogel microparticles with high-resolution and high uniformity

Hyeon Ung Kim, Yong Jun Lim, Hyun Jee Lee, Nak Jun Lee and Ki Wan Bong

Lab Chip, 2020, 20, 74
DOI: 10.1039/C9LC00828D

Oxygen-free discontinuous dewetting in a degassed mold for anisotropic colloidal hydrogel microparticle synthesis

Jiwoo Kim, Jun Hee Choi, Jeongmin Kim, Jihyun Kim, Yoon Ho Roh and Ki Wan Bong

Lab Chip, 2026, 26, 78
DOI: 10.1039/D5LC00928F

Hydrogel Microspheres Empowering Organ‐on‐a‐Chip Systems: Innovations and Applications

Jiacheng Liu, Chengcheng Du, Jinping Chen, Bochen Tang, Senrui Liu, Jianye Tan, Xuefeng Luo, Jingdi Zhan, Junyan Liu, Zhuolin Chen, Yichi Zhang, Zhong Alan Li, Wei Huang and Yiting Lei

Small, 2025, 21
DOI: 10.1002/smll.202504563

Discontinuous Dewetting in a Degassed Mold for Fabrication of Homogeneous Polymeric Microparticles

Hyeon Ung Kim, Yoon Ho Roh, Seok Joon Mun and Ki Wan Bong

ACS Appl. Mater. Interfaces, 2020, 12, 53318
DOI: 10.1021/acsami.0c15944

Synthesis of eco-friendly multifunctional dextran microbeads for multiplexed assays

Jing Zhang, Tao Zheng, Seyed Hossein Helalat, Murat Nulati Yesibolati and Yi Sun

Journal of Colloid and Interface Science, 2024, 666, 603
DOI: 10.1016/j.jcis.2024.04.061

Pushing the detection limits: strategies towards highly sensitive optical-based protein detection

Nikan Momenbeitollahi, Teran Cloet and Huiyan Li

Anal Bioanal Chem, 2021, 413, 5995
DOI: 10.1007/s00216-021-03566-3

A Narrative Review of Bioactive Hydrogel Microspheres: Ingredients, Modifications, Fabrications, Biological Functions, and Applications

Haohui Li, Lili Yu, Ze Li, Sicheng Li, Ye Liu, Guiwen Qu, Kang Chen, Luqiao Huang, Zongan Li, Jianan Ren, Xiuwen Wu and Jinjian Huang

Small, 2025, 21
DOI: 10.1002/smll.202500426

Multiplexed Immunosensors and Immunoarrays

Abby Jones, Lasangi Dhanapala, Rumasha N. T. Kankanamage, Challa V. Kumar and James F. Rusling

Anal. Chem., 2020, 92, 345
DOI: 10.1021/acs.analchem.9b05080

Hydrogel-Based Technologies for the Diagnosis of Skin Pathology

Christian Wiraja, Xiaoyu Ning, Mingyue Cui and Chenjie Xu

Technologies, 2020, 8, 47
DOI: 10.3390/technologies8030047

High-Resolution Patterning of Hydrogel Sensing Motifs within Fibrous Substrates for Sensitive and Multiplexed Detection of Biomarkers

Dana Al Sulaiman, Sarah J. Shapiro, Jose Gomez-Marquez and Patrick S. Doyle

ACS Sens., 2021, 6, 203
DOI: 10.1021/acssensors.0c02121

Elimination of Unreacted Acrylate Double Bonds in the Polymer Networks of Microparticles Synthesized via Flow Lithography

Hyun June Moon, Minhee Ku, Yoon Ho Roh, Hyun Jee Lee, Jaemoon Yang and Ki Wan Bong

Langmuir, 2020, 36, 2271
DOI: 10.1021/acs.langmuir.9b02737

Multiplex SNP Genotyping Using SWITCH: Sequence‐Specific Nanoparticle with Interpretative Toehold‐Mediated Sequence Decoding in Hydrogel

Woongsun Choi, Eunhye Park, Seojin Bae, Kyung‐Hak Choi, Sangeun Han, Kuk‐Hui Son, Do Young Lee, Il‐Joo Cho, Hyejeong Seong, Kyo Seon Hwang, Jwa‐Min Nam, Jungkyu Choi, Hyojin Lee and Nakwon Choi

Small, 2022, 18
DOI: 10.1002/smll.202105538

Development of a CMOS Dielectrophoresis and Microbead-Based Capacitive Immunosensor Array

Hsuan-Ping Chen, Chia-Min Yang and Michael S.-C. Lu

IEEE Sensors J., 2024, 24, 23454
DOI: 10.1109/JSEN.2024.3409432

Multiplexed immunosensors for point-of-care diagnostic applications

Bruno Gil Rosa, Oluwatomi E. Akingbade, Xiaotong Guo, Laura Gonzalez-Macia, Michael A. Crone, Loren P. Cameron, Paul Freemont, Kwang-Leong Choy, Firat Güder, Eric Yeatman, David J. Sharp and Bing Li

Biosensors and Bioelectronics, 2022, 203, 114050
DOI: 10.1016/j.bios.2022.114050

Optimization of particle rinsing process in linker-free post-synthesis functionalization for sensitive encoded hydrogel microparticle-based immunoassay

Su Hyeon Bae, Wookyoung Jang, Jun Hee Choi, Seok Joon Mun and Ki Wan Bong

Journal of Industrial and Engineering Chemistry, 2023, 117, 414
DOI: 10.1016/j.jiec.2022.10.029

Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels

Sang-Wook Bae, Jiyun Kim and Sunghoon Kwon

IJMS, 2022, 23, 2955
DOI: 10.3390/ijms23062955

A New Rapid Microfluidic Detection Platform Utilizing Hydrogel‐Membrane under Cross‐Flow

Adrian T. Nash, Daniel A. N. Foster, Shyan I. Thompson, Seungyoon Han, Madison K. Fernandez and Dae Kun Hwang

Adv Materials Technologies, 2022, 7
DOI: 10.1002/admt.202101396

Highly Flexible Deep-Learning-Based Automatic Analysis for Graphically Encoded Hydrogel Microparticles

Jun Hee Choi, Wookyoung Jang, Yong Jun Lim, Seok Joon Mun and Ki Wan Bong

ACS Sens., 2023, 8, 3158
DOI: 10.1021/acssensors.3c00857

Optimizing reduced capture antibody conjugation to encoded hydrogel microparticles for enhanced multiplex immunoassays

Do Yeon Kim, Jiwoo Kim, Wookyoung Jang and Ki Wan Bong

Front. Sens., 2022, 3
DOI: 10.3389/fsens.2022.1007355

Label-free multiplexed protein quantification in clinical samples using encodable hydrogel barcode and low-aspect-ratio micropore

Chang-Woo Song, Wookyoung Jang, Jinhwa Hong, Soon Young Lim, Dong ge ra mi Moon, Hye Yeon Roh, Kyong Hwa Park, Ki Wan Bong and Chang-Soo Han

Sensors and Actuators B: Chemical, 2025, 432, 137496
DOI: 10.1016/j.snb.2025.137496

Piezoelectric Immunosensor for the Determination of C-Reactive Protein

Miroslav Pohanka

International Journal of Electrochemical Science, 2019, 14, 8470
DOI: 10.20964/2019.09.02

Real-Time Signal Analysis with Wider Dynamic Range and Enhanced Sensitivity in Multiplex Colorimetric Immunoassays Using Encoded Hydrogel Microparticles

Yong Jun Lim, Jun Hee Choi, Seok Joon Mun, Jiwoo Kim and Ki Wan Bong

Anal. Chem., 2024, 96, 7204
DOI: 10.1021/acs.analchem.4c00773

Encoded hydrogel microparticles with universal mismatch-incorporated DNA probes for highly specific multiplex detection of SNPs

Hyun June Moon, Seok Joon Mun, Jun Ho Lee, Yoon Ho Roh, Yong Jun Lim and Ki Wan Bong

Talanta, 2022, 245, 123480
DOI: 10.1016/j.talanta.2022.123480

Dielectrophoresis assisted high-throughput detection system for multiplexed immunoassays

Shih-Mo Yang, Qiang Lin, Hongbo Zhang, Ruixue Yin, Wenjun Zhang, Minchao Zhang and Yubao Cui

Biosensors and Bioelectronics, 2021, 180, 113148
DOI: 10.1016/j.bios.2021.113148

Recent advances in suspension microarray-based multiplexed biosensing technologies integrated with fluorescence signal amplification

Wookyoung Jang, E Loomee Song and Ki Wan Bong

TrAC Trends in Analytical Chemistry, 2025, 192, 118397
DOI: 10.1016/j.trac.2025.118397

Direct Conjugation of Streptavidin to Encoded Hydrogel Microparticles for Multiplex Biomolecule Detection with Rapid Probe-Set Modification

Yoon Ho Roh, Ju Yeon Kim, Seok Joon Mun, Hye Sun Lee, Changhyun Hwang, Kyong Hwa Park and Ki Wan Bong

Polymers, 2020, 12, 546
DOI: 10.3390/polym12030546

Microfluidic fabrication of fatty alcohol-based microparticles for NIR light-triggered drug release

Hyeon Ung Kim, Yoon Ho Roh, Min Suk Shim and Ki Wan Bong

Journal of Industrial and Engineering Chemistry, 2019, 80, 778
DOI: 10.1016/j.jiec.2019.06.047

Micropore device for identification of 4-bit hydrogel barcode

Jihun Ryu, Hyeon Ung Kim, Chang-Woo Song, Sehyun Shin, Ki Wan Bong and Chang-Soo Han

Sensors and Actuators B: Chemical, 2020, 307, 127622
DOI: 10.1016/j.snb.2019.127622

Encoded Hydrogel Microparticles with Universal Mismatch DNA Probes for Highly Specific Multiplex Detection of Snps

Hyun June Moon, Seok Joon Mun, Jun Ho Lee, Yoon Ho Roh, Yong Jun Lim and Ki Wan Bong

SSRN Journal, 2022
DOI: 10.2139/ssrn.4017133

Exploiting cellulose-based hydrogels for sustainable, intelligent wearables in pandemic preparedness and control

Kindness A. Uyanga, Wanxin Li and Walid A. Daoud

European Polymer Journal, 2024, 212, 113041
DOI: 10.1016/j.eurpolymj.2024.113041

Efficient isolation of encoded microparticles in a degassed micromold for highly sensitive and multiplex immunoassay with signal amplification

Wookyoung Jang, E Loomee Song, Seok Joon Mun and Ki Wan Bong

Biosensors and Bioelectronics, 2024, 261, 116465
DOI: 10.1016/j.bios.2024.116465

Direct functionalization of cell‐adhesion promoters to hydrogel microparticles synthesized by stop‐flow lithography

Wookyoung Jang, Do Yeon Kim, Seok Joon Mun, Jun Hee Choi, Yoon Ho Roh and Ki Wan Bong

Journal of Polymer Science, 2022, 60, 1767
DOI: 10.1002/pol.20210934

Microfluidic production of polyacrylic acid functionalized PEG microgels for efficient biomolecular conjugation

Yoon Choi, Su-Ryeon Park, Sei-Jung Lee and Chang-Hyung Choi

Front. Sens., 2022, 3
DOI: 10.3389/fsens.2022.1016791

Hydrogel-Based In Situ DNA Extension Assay for Multiplexed and Rapid Detection of MicroRNA

Wookyoung Jang, Yu Jin Kim, Hyun Kyo Roh, E Loomee Song and Ki Wan Bong

Anal. Chem., 2025, 97, 7317
DOI: 10.1021/acs.analchem.4c06995