Francisco Conceição, Nuno Araújo-Gomes, Johanna F. A. Husch, Malin Becker, Jeroen J. J. P. van den Beucken, Jeroen Leijten and Liliana Moreira Teixeira

Lab Chip, 2026, 26, 331
DOI: 10.1039/D5LC00682A

Microfluidic harvesting of breast cancer tumor spheroid-derived extracellular vesicles from immobilized microgels for single-vesicle analysis

Xilal Y. Rima, Jingjing Zhang, Luong T. H. Nguyen, Aaron Rajasuriyar, Min Jin Yoon, Chi-Ling Chiang, Nicole Walters, Kwang Joo Kwak, L. James Lee and Eduardo Reátegui

Lab Chip, 2022, 22, 2502
DOI: 10.1039/D1LC01053K

Microfluidic fabrication of microparticles for biomedical applications

Wen Li, Liyuan Zhang, Xuehui Ge, Biyi Xu, Weixia Zhang, Liangliang Qu, Chang-Hyung Choi, Jianhong Xu, Afang Zhang, Hyomin Lee and David A. Weitz

Chem. Soc. Rev., 2018, 47, 5646
DOI: 10.1039/C7CS00263G

Core–Shell Particles: From Fabrication Methods to Diverse Manipulation Techniques

Ajeet Singh Yadav, Du Tuan Tran, Adrian J. T. Teo, Yuchen Dai, Fariba Malekpour Galogahi, Chin Hong Ooi and Nam-Trung Nguyen

Micromachines, 2023, 14, 497
DOI: 10.3390/mi14030497

All the single cells: if you like it then you should put some virus on it

Sophia K. Adams, Grace E. Ducharme, Emma K. Loveday and Suchetana Mukhopadhyay

J Virol, 2024, 98
DOI: 10.1128/jvi.01273-23

A Machine Learning and Computer Vision Approach to Rapidly Optimize Multiscale Droplet Generation

Alexander E. Siemenn, Evyatar Shaulsky, Matthew Beveridge, Tonio Buonassisi, Sara M. Hashmi and Iddo Drori

ACS Appl. Mater. Interfaces, 2022, 14, 4668
DOI: 10.1021/acsami.1c19276

Geometric screening of core/shell hydrogel microcapsules using a tapered microchannel with interdigitated electrodes

Ye Niu, Lin Qi, Fen Zhang and Yi Zhao

Biosensors and Bioelectronics, 2018, 112, 162
DOI: 10.1016/j.bios.2018.04.040

Controllable Fabrication of Composite Core–Shell Capsules at a Macroscale as Organoid Biocarriers

Fan He, Tingting Tao, Haitao Liu, Yaqing Wang, Kangli Cui, Yaqiong Guo and Jianhua Qin

ACS Appl. Bio Mater., 2021, 4, 1584
DOI: 10.1021/acsabm.0c01441

Synthesis and active manipulation of magnetic liquid beads

Ajeet Singh Yadav, Fariba Malekpour Galogahi, Aditya Vashi, Du Tuan Tran, Gregor S. Kijanka, Haotian Cha, Kamalalayam Rajan Sreejith and Nam-Trung Nguyen

Biomed Microdevices, 2024, 26
DOI: 10.1007/s10544-024-00708-z

Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering

Katarzyna O. Rojek, Monika Ćwiklińska, Julia Kuczak and Jan Guzowski

Chem. Rev., 2022, 122, 16839
DOI: 10.1021/acs.chemrev.1c00798

Droplet Microfluidics for Current Cancer Research: From Single-Cell Analysis to 3D Cell Culture

Lin Jiang, Kefan Guo, Yao Chen and Nan Xiang

ACS Biomater. Sci. Eng., 2024, 10, 1335
DOI: 10.1021/acsbiomaterials.3c01866

A Flexible, Microfluidic, Dispensing System for Screening Drug Combinations

Mark Davies, Mannthalah Abubaker and Lorraine Bible

Micromachines, 2020, 11, 943
DOI: 10.3390/mi11100943

The cell-loaded alginate microspheres in cell culture and disease treatment

Meng-Yuan Wang, Han-Lin Tao, Jun Li, Xue-Yu Chen, Ya-Chao Gu, Ruizhi Tang and Xi-Qiu Liu

Smart Materials in Medicine, 2025, 6, 387
DOI: 10.1016/j.smaim.2025.11.002

3D Cell Culture—Can It Be As Popular as 2D Cell Culture?

Miao Sun, An Liu, Xiaofu Yang, Jiaxing Gong, Mengfei Yu, Xinhua Yao, Huiming Wang and Yong He

Advanced NanoBiomed Research, 2021, 1
DOI: 10.1002/anbr.202000066

Dynamic Poly(amine) Capsules with Reversible pH-Triggered Responsiveness

Zhang Wu, Jörg G. Werner and David A. Weitz

ACS Appl. Polym. Mater., 2025, 7, 7893
DOI: 10.1021/acsapm.5c00819

Dynamics of capsules enclosing viscoelastic fluid in simple shear flow

Zheng Yuan Luo and Bo Feng Bai

J. Fluid Mech., 2018, 840, 656
DOI: 10.1017/jfm.2018.88

Nanoliter Hydrogel Array for Cell Screening and Cell Spheroid Sorting

Maryam Salarian, Pavel A. Levkin and Anna A. Popova

Adv Materials Technologies, 2025, 10
DOI: 10.1002/admt.202401159

Microencapsulated Immunoassays for Detection of Cytokines in Human Blood

Ali Rahimian, Christian Siltanen, Hamid Feyzizarnagh, Patricio Escalante and Alexander Revzin

ACS Sens., 2019, 4, 578
DOI: 10.1021/acssensors.8b01033

Design of an Adhesive Film-Based Microfluidic Device for Alginate Hydrogel-Based Cell Encapsulation

Kevin Enck, Shiny Priya Rajan, Julio Aleman, Simone Castagno, Emily Long, Fatma Khalil, Adam R. Hall and Emmanuel C. Opara

Ann Biomed Eng, 2020, 48, 1103
DOI: 10.1007/s10439-020-02453-9

Oil-Free Acoustofluidic Droplet Generation for Multicellular Tumor Spheroid Culture

Jacqueline A. De Lora, Frank A. Fencl, Aidira D.Y. Macias Gonzalez, Alireza Bandegi, Reza Foudazi, Gabriel P. Lopez, Andrew P. Shreve and Nick J. Carroll

ACS Appl. Bio Mater., 2019, 2, 4097
DOI: 10.1021/acsabm.9b00617

Biomass Microcapsules with Stem Cell Encapsulation for Bone Repair

Lei Yang, Yuxiao Liu, Lingyu Sun, Cheng Zhao, Guopu Chen and Yuanjin Zhao

Nano-Micro Lett., 2022, 14
DOI: 10.1007/s40820-021-00747-8

Continuous and controllable synthesis of MnO2/PPy composites with core–shell structures for supercapacitors

Yang Song, Minjing Shang, Junguo Li and Yuanhai Su

Chemical Engineering Journal, 2021, 405, 127059
DOI: 10.1016/j.cej.2020.127059

From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts

Jolien Breukers, Karen Ven, Wannes Verbist, Iene Rutten and Jeroen Lammertyn

Biomicrofluidics, 2025, 19
DOI: 10.1063/5.0242599

Multiphase Microfluidic Processes to Produce Alginate-Based Microparticles and Fibers

Masumi Yamada and Minoru Seki

Journal of Chemical Engineering of Japan, 2018, 51, 318
DOI: 10.1252/jcej.17we328

Hydrodynamic Snaring Array for Trapping and Perfusion Culture of Single Cell

Ya-Yu Chiang, Yi-Chiuan Teng, Zhe-Yi Su, Han-Yu Hsueh and Kai-Hung Tu

Sensors and Actuators B: Chemical, 2020, 312, 127966
DOI: 10.1016/j.snb.2020.127966

Trends in Droplet Microfluidics: From Droplet Generation to Biomedical Applications

Zhengkun Chen, Sina Kheiri, Edmond W. K. Young and Eugenia Kumacheva

Langmuir, 2022, 38, 6233
DOI: 10.1021/acs.langmuir.2c00491

Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances

Hazal Kutluk, Martina Viefhues and Iordania Constantinou

Small Science, 2024, 4
DOI: 10.1002/smsc.202300206

A Microfluidic Detection System for Bladder Cancer Tumor Cells

Shuxing Lv, Jinwei Yu, Yan Zhao, Hongxiang Li, Fang Zheng, Ning Liu, Dahua Li and Xuguo Sun

Micromachines, 2019, 10, 871
DOI: 10.3390/mi10120871

Recent advances in microfluidic technology for manipulation and analysis of biological cells (2007–2017)

Md Kowsar Alam, Emmanuel Koomson, Heng Zou, Changqing Yi, Cheuk-Wing Li, Tao Xu and Mengsu Yang

Analytica Chimica Acta, 2018, 1044, 29
DOI: 10.1016/j.aca.2018.06.054

Microfluidics Enabling the Controlled Manufacturing of Peptide and Protein Micro and Nano Biomaterials for Biomedical Applications

Gaia Pascolo, Bekzod Bekchanov, Mohamed A. N. Soliman, Eugenia Spessot, Hafiz Ali, Omar Qutachi, Meisam Abdi, Annalisa Tirella and Mohamed A. Elsawy

Biomacromolecules, 2025, 26, 3903
DOI: 10.1021/acs.biomac.4c01712

Engineering Strategies to Improve Islet Transplantation for Type 1 Diabetes Therapy

Alisa M. White, James G. Shamul, Jiangsheng Xu, Samantha Stewart, Jonathan S. Bromberg and Xiaoming He

ACS Biomater. Sci. Eng., 2020, 6, 2543
DOI: 10.1021/acsbiomaterials.9b01406

High accuracy size-based droplet separation with pinched flow fractionation

Xun Liu, Doudou Ma, Yapeng Yuan, Tao Tang, Yoichiroh Hosokawa and Yaxiaer Yalikun

Appl. Phys. Express, 2023, 16, 116502
DOI: 10.35848/1882-0786/ad0b41

Hydrogels as functional components in artificial cell systems

Matthew E. Allen, James W. Hindley, Divesh K. Baxani, Oscar Ces and Yuval Elani

Nat Rev Chem, 2022, 6, 562
DOI: 10.1038/s41570-022-00404-7

Geometry Effects of Axisymmetric Flow-Focusing Microchannels for Single Cell Encapsulation

Mohammad Nooranidoost and Ranganathan Kumar

Materials, 2019, 12, 2811
DOI: 10.3390/ma12172811

Biofabrication strategies with single-cell resolution: a review

Dezhi Zhou, Bohan Dou, Florian Kroh, Chuqian Wang and Liliang Ouyang

Int. J. Extrem. Manuf., 2023, 5, 042005
DOI: 10.1088/2631-7990/ace863

An arbitrary Lagrangian-Eulerian method for simulating interfacial dynamics between a hydrogel and a fluid

Lei Li, Jiaqi Zhang, Zelai Xu, Y.-N. Young, James J. Feng and Pengtao Yue

Journal of Computational Physics, 2022, 451, 110851
DOI: 10.1016/j.jcp.2021.110851

Designing Tough Hydrogel Shells for Glucose Sensing

Yeounju Ko, Yoonjin Oh, Chan Ho Park and Shin‐Hyun Kim

Small, 2024, 20
DOI: 10.1002/smll.202310283

Programmable higher-order biofabrication of self-locking microencapsulation

Yang Liu, Cong Wu, Haojian Lu, Yuanyuan Yang, Wenjung Li and Yajing Shen

Biofabrication, 2019, 11, 035019
DOI: 10.1088/1758-5090/aafd14

Breakups of Chitosan microcapsules in extensional flow

Revaz Chachanidze, Kaili Xie, Jinming Lyu, Marc Jaeger and Marc Leonetti

Journal of Colloid and Interface Science, 2023, 629, 445
DOI: 10.1016/j.jcis.2022.08.169

Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering

Yufan Zhang, Panjing Yin, Junfei Huang, Lunan Yang, Zhen Liu, Danlan Fu, Zhiqi Hu, Wenhua Huang and Yong Miao

J Nanobiotechnol, 2022, 20
DOI: 10.1186/s12951-022-01671-8

An acoustofluidic platform for non-contact trapping of cell-laden hydrogel droplets compatible with optical microscopy

Anna Fornell, Carl Johannesson, Sean S. Searle, Axel Happstadius, Johan Nilsson and Maria Tenje

Biomicrofluidics, 2019, 13
DOI: 10.1063/1.5108583

Noncovalent reversible binding-enabled facile fabrication of leak-free PDMS microfluidic devices without plasma treatment for convenient cell loading and retrieval

Bin Jiang, Alisa White, Wenquan Ou, Sarah Van Belleghem, Samantha Stewart, James G. Shamul, Shaik O. Rahaman, John P. Fisher and Xiaoming He

Bioactive Materials, 2022, 16, 346
DOI: 10.1016/j.bioactmat.2022.02.031

Enhanced single-cell encapsulation in microfluidic devices: From droplet generation to single-cell analysis

Si Da Ling, Yuhao Geng, An Chen, Yanan Du and Jianhong Xu

Biomicrofluidics, 2020, 14, 061508
DOI: 10.1063/5.0018785

Oral delivery of bacteria: Basic principles and biomedical applications

Shuqi Li, Weiqian Jiang, Chunxiong Zheng, Dan Shao, Yilun Liu, Shuqiang Huang, Jing Han, Jianxun Ding, Yu Tao and Mingqiang Li

Journal of Controlled Release, 2020, 327, 801
DOI: 10.1016/j.jconrel.2020.09.011

Designing Semipermeable Hydrogel Shells with Controlled Thickness through Internal Osmosis in Triple‐Emulsion Droplets

Sangmin Lee, Biao Che, Meiling Tai, Wanzhao Li and Shin‐Hyun Kim

Adv Funct Materials, 2021, 31
DOI: 10.1002/adfm.202105477

Upscaling Osteoclast Generation by Enhancing Macrophage Aggregation Using Hollow Microgels

Johanna F.A. Husch, Nuno Araújo‐Gomes, Niels G.A. Willemen, Carla Cofiño‐Fabrés, Nils van Creij, Robert Passier, Jeroen Leijten and Jeroen J. J. P. van den Beucken

Small, 2024, 20
DOI: 10.1002/smll.202403272

Encapsulation of Cells in a Collagen Matrix Surrounded by an Alginate Hydrogel Shell for 3D Cell Culture

Wafa Bouhlel, Jessica Kui, Jérôme Bibette and Nicolas Bremond

ACS Biomater. Sci. Eng., 2022, 8, 2700
DOI: 10.1021/acsbiomaterials.1c01486

Recent advances in microfluidic technologies for organ-on-a-chip

Chang Tian, Qin Tu, Wenming Liu and Jinyi Wang

TrAC Trends in Analytical Chemistry, 2019, 117, 146
DOI: 10.1016/j.trac.2019.06.005

Crosslinking Strategies for the Microfluidic Production of Microgels

Minjun Chen, Guido Bolognesi and Goran T. Vladisavljević

Molecules, 2021, 26, 3752
DOI: 10.3390/molecules26123752

Rapid Production and Recovery of Cell Spheroids by Automated Droplet Microfluidics

Krzysztof Langer and Haakan N. Joensson

SLAS Technology, 2020, 25, 111
DOI: 10.1177/2472630319877376

Innovations in 3D ovarian and follicle engineering for fertility preservation and restoration

Negin Chavoshinezhad and Behrooz Niknafs

Mol Biol Rep, 2024, 51
DOI: 10.1007/s11033-024-09783-0

Droplet-based microfluidics in biomedical applications

Leyla Amirifar, Mohsen Besanjideh, Rohollah Nasiri, Amir Shamloo, Fatemeh Nasrollahi, Natan Roberto de Barros, Elham Davoodi, Ahmet Erdem, Mahboobeh Mahmoodi, Vahid Hosseini, Hossein Montazerian, Jamileh Jahangiry, Mohammad Ali Darabi, Reihaneh Haghniaz, Mehmet R Dokmeci, Nasim Annabi, Samad Ahadian and Ali Khademhosseini

Biofabrication, 2022, 14, 022001
DOI: 10.1088/1758-5090/ac39a9

C3PE: counter-current continuous phase extraction for improved precision of in-droplet chemical reactions

Steven R. Doonan, Melissa Lin, Dongkwan Lee, Jamy Lee and Ryan C. Bailey

Microfluid Nanofluid, 2020, 24
DOI: 10.1007/s10404-020-02354-2

Advances in microfabrication technologies in tissue engineering and regenerative medicine

Sara Nadine, Ada Chung, Sibel Emir Diltemiz, Brooke Yasuda, Charles Lee, Vahid Hosseini, Solmaz Karamikamkar, Natan Roberto de Barros, Kalpana Mandal, Shailesh Advani, Benjamin Behnam Zamanian, Marvin Mecwan, Yangzhi Zhu, Mohammad Mofidfar, Mohammad Reza Zare, João Mano, Mehmet Remzi Dokmeci, Farshid Alambeigi and Samad Ahadian

Artificial Organs, 2022, 46
DOI: 10.1111/aor.14232

Engineered biomimetic micro/nano-materials for tissue regeneration

Feng Han, Qingchen Meng, En Xie, Kexin Li, Jie Hu, Qianglong Chen, Jiaying Li and Fengxuan Han

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1205792

DoE Analysis of Approaches for Hydrogel Microbeads’ Preparation by Millifluidic Methods

Anna Nastruzzi, Gabriele Pitingolo, Giovanni Luca and Claudio Nastruzzi

Micromachines, 2020, 11, 1007
DOI: 10.3390/mi11111007

Engineering biomaterials by inkjet printing of hydrogels with functional particulates

Cih Cheng, Eric J Williamson, George T.-C. Chiu and Bumsoo Han

Med-X, 2024, 2
DOI: 10.1007/s44258-024-00024-4

Electrically controllable cargo delivery with dextran-rich droplets

Mengqi Li and Dongqing Li

Journal of Colloid and Interface Science, 2021, 582, 102
DOI: 10.1016/j.jcis.2020.08.033

Probing Single-Cell Macrophage Polarization and Heterogeneity Using Thermo-Reversible Hydrogels in Droplet-Based Microfluidics

B. M. Tiemeijer, M. W. D. Sweep, J. J. F. Sleeboom, K. J. Steps, J. F. van Sprang, P. De Almeida, R. Hammink, P. H. J. Kouwer, A. I. P. M. Smits and J. Tel

Front. Bioeng. Biotechnol., 2021, 9
DOI: 10.3389/fbioe.2021.715408

Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

Marco Costantini, Jan Guzowski, Paweł J. Żuk, Pamela Mozetic, Simone De Panfilis, Jakub Jaroszewicz, Marcin Heljak, Mara Massimi, Maxime Pierron, Marcella Trombetta, Mariella Dentini, Wojciech Święszkowski, Alberto Rainer, Piotr Garstecki and Andrea Barbetta

Adv Funct Materials, 2018, 28
DOI: 10.1002/adfm.201800874

Organ-on-a-Chip Models of the Female Reproductive System: Current Progress and Future Perspectives

Min Pan, Huike Chen, Kai Deng and Ke Xiao

Micromachines, 2025, 16, 1125
DOI: 10.3390/mi16101125

Easy-to-Operate Co-Flow Step Emulsification Device for High-Throughput Three-Dimensional Cell Culture

Chunyang Wei, Chengzhuang Yu, Shanshan Li, Tiejun Li, Jiyu Meng and Junwei Li

Biosensors, 2022, 12, 350
DOI: 10.3390/bios12050350

Boundary conditions at a gel-fluid interface

James J. Feng and Y.-N. Young

Phys. Rev. Fluids, 2020, 5
DOI: 10.1103/PhysRevFluids.5.124304

Computational Modelling and Big Data Analysis of Flow and Drug Transport in Microfluidic Systems: A Spheroid-on-a-Chip Study

Sina Kheiri, Eugenia Kumacheva and Edmond W.K. Young

Front. Bioeng. Biotechnol., 2021, 9
DOI: 10.3389/fbioe.2021.781566

Recent Advances in Microfluidic Platforms for Programming Cell‐Based Living Materials

Pengchao Zhang, Ning Shao and Lidong Qin

Advanced Materials, 2021, 33
DOI: 10.1002/adma.202005944

Programmable pulsed aerodynamic printing for multi-interface composite manufacturing

Zhiqiang Zhu, Tianao Chen, Yuanqing Zhu, Fangsheng Huang, Kai Mu, Ting Si and Ronald X. Xu

Matter, 2023, 6, 2034
DOI: 10.1016/j.matt.2023.04.017

One-step biofabrication of liquid core—GelMa shell microbeads for in situ hollow cell ball self-assembly

Jianwei Chen, Zeyang Liu, Zixian Wang, Xiuxiu Zhang, Yi Zhang, Zhen Zhan, Xiaohua Gong and Tao Xu

Regenerative Biomaterials, 2024, 11
DOI: 10.1093/rb/rbae021

Microfluidic and cross-linking methods for encapsulation of living cells and bacteria - A review

T. Alkayyali, T. Cameron, B. Haltli, R.G. Kerr and A. Ahmadi

Analytica Chimica Acta, 2019, 1053, 1
DOI: 10.1016/j.aca.2018.12.056

Controllable and Scaffold‐Free Formation of 3D Multicellular Architectures Using a Bipolar Electrode Array

Yupan Wu, Haohao Zhang, Yuanbo Yue, Shaoxi Wang and Yingqi Meng

Adv Materials Technologies, 2024, 9
DOI: 10.1002/admt.202301626

Recent Advances in Droplet-based Microfluidic Technologies for Biochemistry and Molecular Biology

Joel Sánchez Barea, Juhwa Lee and Dong-Ku Kang

Micromachines, 2019, 10, 412
DOI: 10.3390/mi10060412

How Microgels Can Improve the Impact of Organ-on-Chip and Microfluidic Devices for 3D Culture: Compartmentalization, Single Cell Encapsulation and Control on Cell Fate

Simona Argentiere, Pietro Aleardo Siciliano and Laura Blasi

Polymers, 2021, 13, 3216
DOI: 10.3390/polym13193216

Implementation of blood-brain barrier on microfluidic chip: Recent advance and future prospects

Wangyu Bi, Shanglin Cai, Tong Lei and Lei Wang

Ageing Research Reviews, 2023, 87, 101921
DOI: 10.1016/j.arr.2023.101921

Superimposed Electric Field Enhanced Electrospray for High‐Throughput and Consistent Cell Encapsulation

Zejun Fan, Yihan Chen, Zhen Yang, Yudi Niu, Kaini Liang, Yan Zhang, Jianan Zeng, Yiting Feng, Yuying Zhang, Ye Liu, Cheng Lv, Peng Zhao, Lv Zhou, Wenyu Kong, Wenjing Li, Haoke Chen, Dongbo Han and Yanan Du

Adv Healthcare Materials, 2024, 13
DOI: 10.1002/adhm.202400780

Droplets can enhance microcapsule deformation in channel flow

Dalei Jing, Ruixin Lu, Alexander Farutin, Ziyu Guo, Fan Wang, Wen Wang, Chaouqi Misbah and Yi Sui

Commun Phys, 2024, 7
DOI: 10.1038/s42005-024-01805-4

Centrifugal microfluidics for ultra-rapid fabrication of versatile hydrogel microcarriers

Yue Cheng, Xiaozhang Zhang, Yuan Cao, Conghui Tian, Yufang Li, Meng Wang, Yuanjin Zhao and Gang Zhao

Applied Materials Today, 2018, 13, 116
DOI: 10.1016/j.apmt.2018.08.012

Acoustic focusing of beads and cells in hydrogel droplets

Anna Fornell, Hannah Pohlit, Qian Shi and Maria Tenje

Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-86985-7

Spherical Magnetic Fe-Alginate Microgels Fabricated by Droplet-Microfluidics Combining with an External Crosslinking Approach and the Study of Their pH Dependent Fe3+ Release Behaviors

Jie Chen, Run-Yu Yu, Kai-Qi Wang, Zhe-Yu Zhang, Arezoo Ardekani and Yuan-Du Hu

Chin J Polym Sci, 2025, 43, 289
DOI: 10.1007/s10118-025-3257-2

Recent advances in engineering approaches for delivery of mRNA-based therapeutics

Ken Ninez Nurpramesti Prinindya, Ghulam Md Ashraf, Shihab Uddin, Elsadig Mahdi and Anwarul Hasan

Biomaterials Advances, 2026, 182, 214667
DOI: 10.1016/j.bioadv.2025.214667

Droplets microfluidics platform—A tool for single cell research

Bixuan Li, Xi Ma, Jianghong Cheng, Tian Tian, Jiao Guo, Yang Wang and Long Pang

Front. Bioeng. Biotechnol., 2023, 11
DOI: 10.3389/fbioe.2023.1121870

Engineering organoid microfluidic system for biomedical and health engineering: A review

Yifan Xing, Junyu Liu, Xiaojie Guo, Haipeng Liu, Wen Zeng, Yi Wang, Chong Zhang, Yuan Lu, Dong He, Shaohua Ma, Yonghong He and Xin-Hui Xing

Chinese Journal of Chemical Engineering, 2021, 30, 244
DOI: 10.1016/j.cjche.2020.11.013

Effects of geometry factors on microvortices evolution in confined square microcavities

Feng Shen, Min Xu, Bin Zhou, Zheng Wang and Zhaomiao Liu

Microfluid Nanofluid, 2018, 22
DOI: 10.1007/s10404-018-2056-2

Advances in high throughput cell culture technologies for therapeutic screening and biological discovery applications

Hyeon Ryoo, Hannah Kimmel, Evi Rondo and Gregory H. Underhill

Bioengineering & Transla Med, 2024, 9
DOI: 10.1002/btm2.10627

Jammed Microgel Inks for 3D Printing Applications

Christopher B. Highley, Kwang Hoon Song, Andrew C. Daly and Jason A. Burdick

Advanced Science, 2019, 6
DOI: 10.1002/advs.201801076

Rapid construct superhydrophobic microcracks on the open-surface platform for droplet manipulations

Wan-Hsuan Lin, Chien-Wei Chen, Sheng-Hang Wang and Bor-Ran Li

Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-94484-y

Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture

Sung-Min Kang, Ji-Hoon Lee, Yun Suk Huh and Shuichi Takayama

ACS Biomater. Sci. Eng., 2021, 7, 2864
DOI: 10.1021/acsbiomaterials.0c00457

Microfluidics-based fabrication of cell-laden microgels

Mohamed G. A. Mohamed, Pranav Ambhorkar, Roya Samanipour, Annie Yang, Ali Ghafoor and Keekyoung Kim

Biomicrofluidics, 2020, 14
DOI: 10.1063/1.5134060

Recent progress in high-throughput droplet screening and sorting for bioanalysis

Guoyun Sun, Lisha Qu, Fidelis Azi, Yanfeng Liu, Jianghua Li, Xueqin Lv, Guocheng Du, Jian Chen, Chia-Hung Chen and Long Liu

Biosensors and Bioelectronics, 2023, 225, 115107
DOI: 10.1016/j.bios.2023.115107

Necklace‐Like Microfibers with Variable Knots and Perfusable Channels Fabricated by an Oil‐Free Microfluidic Spinning Process

Ruoxiao Xie, Peidi Xu, Yupeng Liu, Lili Li, Guoan Luo, Mingyu Ding and Qionglin Liang

Advanced Materials, 2018, 30
DOI: 10.1002/adma.201705082

Spheroid Formation and Recovery Using Superhydrophobic Coating for Regenerative Purposes

María Morán, Francesca Cirisano and Michele Ferrari

Pharmaceutics, 2023, 15, 2226
DOI: 10.3390/pharmaceutics15092226

High‐Throughput Encapsulation of Stem Cells: Characterizing Dynamic Culture Variability With a Millifluidic Approach

Oscar Fabian García‐Aponte, Marta Serra, Simon Kahlenberg, Guruprakash Subbiahdoss, Erik Reimhult, Dominik Egger and Cornelia Kasper

Adv Healthcare Materials, 2025, 14
DOI: 10.1002/adhm.202405137

Controllable fabrication of millimeter-scale double droplets in co-flowing devices

Xiaoyu Yang, Meifang Liu, Jie Li, Qiang Chen, Yiyang Liu, Lin Yan, Xianyi Jiang and Hao Liu

Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2023, 662, 130978
DOI: 10.1016/j.colsurfa.2023.130978

Microgels based on 0D-3D carbon materials: Synthetic techniques, properties, applications, and challenges

Vinh Van Tran, Eunsol Wi, Seo Young Shin, Daeho Lee, Yoong Ahm Kim, Byung Chol Ma and Mincheol Chang

Chemosphere, 2022, 307, 135981
DOI: 10.1016/j.chemosphere.2022.135981

Mammalian cell encapsulation in monodisperse chitosan beads using microchannel emulsification

Dongjin S. Shin, Francesco K. Touani, Damon G.K. Aboud, Anne-Marie Kietzig, Sophie Lerouge and Corinne A. Hoesli

Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2023, 661, 130807
DOI: 10.1016/j.colsurfa.2022.130807

Cell‐Surface Engineering for Advanced Cell Therapy

Jungkyu K. Lee, Insung S. Choi, Tong In Oh and EunAh Lee

Chemistry A European J, 2018, 24, 15725
DOI: 10.1002/chem.201801710

Droplet Generation and Manipulation in Microfluidics: A Comprehensive Overview of Passive and Active Strategies

Andrea Fergola, Alberto Ballesio, Francesca Frascella, Lucia Napione, Matteo Cocuzza and Simone Luigi Marasso

Biosensors, 2025, 15, 345
DOI: 10.3390/bios15060345

Continuous microfluidic encapsulation of single mesenchymal stem cells using alginate microgels as injectable fillers for bone regeneration

Chuanfeng An, Weijian Liu, Yang Zhang, Bo Pang, Hui Liu, Yujie Zhang, Haoyue Zhang, Liyuan Zhang, Hongbing Liao, Changle Ren and Huanan Wang

Acta Biomaterialia, 2020, 111, 181
DOI: 10.1016/j.actbio.2020.05.024

Growth factor loaded in situ photocrosslinkable poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin methacryloyl hybrid patch for diabetic wound healing

Robin Augustine, Anwarul Hasan, Yogesh B. Dalvi, Syed Raza Ur Rehman, Ruby Varghese, Raghunath Narayanan Unni, Huseyin C. Yalcin, Rashad Alfkey, Sabu Thomas and Ala-Eddin Al Moustafa

Materials Science and Engineering: C, 2021, 118, 111519
DOI: 10.1016/j.msec.2020.111519

Highly efficient fabrication of functional hepatocyte spheroids by a magnetic system for the rescue of acute liver failure

Jiabin Zhang, Yanteng Xu, Chenya Zhuo, Run Shi, Haixia Wang, Zuquan Hu, Hon Fai Chan, Hae-Won Kim, Yu Tao and Mingqiang Li

Biomaterials, 2023, 294, 122014
DOI: 10.1016/j.biomaterials.2023.122014

Two-Step Numerical Approach To Predict Ferrofluid Droplet Generation and Manipulation inside Multilaminar Flow Chambers

Jenifer Gómez-Pastora, Venoos Amiri Roodan, Ioannis H. Karampelas, Ali Q. Alorabi, Mark D. Tarn, Alexander Iles, Eugenio Bringas, Vesselin N. Paunov, Nicole Pamme, Edward P. Furlani and Inmaculada Ortiz

J. Phys. Chem. C, 2019, 123, 10065
DOI: 10.1021/acs.jpcc.9b01393

A Droplet Microfluidic System to Fabricate Hybrid Capsules Enabling Stem Cell Organoid Engineering

Haitao Liu, Yaqing Wang, Hui Wang, Mengqian Zhao, Tingting Tao, Xu Zhang and Jianhua Qin

Advanced Science, 2020, 7
DOI: 10.1002/advs.201903739

Transcriptomics in Toxicogenomics, Part I: Experimental Design, Technologies, Publicly Available Data, and Regulatory Aspects

Pia Anneli Sofia Kinaret, Angela Serra, Antonio Federico, Pekka Kohonen, Penny Nymark, Irene Liampa, My Kieu Ha, Jang-Sik Choi, Karolina Jagiello, Natasha Sanabria, Georgia Melagraki, Luca Cattelani, Michele Fratello, Haralambos Sarimveis, Antreas Afantitis, Tae-Hyun Yoon, Mary Gulumian, Roland Grafström, Tomasz Puzyn and Dario Greco

Nanomaterials, 2020, 10, 750
DOI: 10.3390/nano10040750

Recent Developments in 3D Printing of Droplet-Based Microfluidics

Adedamola D. Aladese and Heon-Ho Jeong

BioChip J, 2021, 15, 313
DOI: 10.1007/s13206-021-00032-1

Anti-Inflammatory and Repairing Effects of Mesoporous Silica-Loaded Metronidazole Composite Hydrogel on Human Dental Pulp Cells

Wei Wang, Xixi Wang, Luyang Li, Ying Liu and Bhagyaveni M.A

Journal of Healthcare Engineering, 2022, 2022, 1
DOI: 10.1155/2022/6774075

A correlation of conductivity medium and bioparticle viability on dielectrophoresis‐based biomedical applications

Revathy Deivasigamani, Nur Nasyifa Mohd Maidin, Nur Shahira Abdul Nasir, Abdullah Abdulhameed, Aminuddin Bin Ahmad Kayani, Mohd Ambri Mohamed and Muhamad Ramdzan Buyong

Electrophoresis, 2023, 44, 573
DOI: 10.1002/elps.202200203

Hydrogels for Single-Cell Microgel Production: Recent Advances and Applications

B. M. Tiemeijer and J. Tel

Front. Bioeng. Biotechnol., 2022, 10
DOI: 10.3389/fbioe.2022.891461

Hydrogel-based preparation of cell aggregates for biomedical applications

Jiabin Zhang, Seonho Yun, Yuguang Du, Andrew Zannettino and Hu Zhang

Applied Materials Today, 2020, 20, 100747
DOI: 10.1016/j.apmt.2020.100747

Preparation of mint oil microcapsules by microfluidics with high efficiency and controllability in release properties

Yuhan Du, Liangji Mo, Xiaoda Wang, Hongxing Wang, Xue-hui Ge and Ting Qiu

Microfluid Nanofluid, 2020, 24
DOI: 10.1007/s10404-020-02346-2

High-Throughput Microfluidic Production of Droplets and Hydrogel Microspheres through Monolithically Integrated Microchannel Plates

Boxuan Wu, Xuefeng Xu, Guangyang Li, Xi Yang, Feiya Du, Weiqiang Tan, Jianmin Wang, Shurong Dong, Jikui Luo, Xiaozhi Wang and Zhen Cao

Anal. Chem., 2023, 95, 13586
DOI: 10.1021/acs.analchem.3c02250

Droplet microfluidic devices for organized stem cell differentiation into germ cells: capabilities and challenges

Reyhaneh Sadat Hayaei Tehrani, Mohammad Amin Hajari, Zeynab Ghorbaninejad and Fereshteh Esfandiari

Biophys Rev, 2021, 13, 1245
DOI: 10.1007/s12551-021-00907-5

Droplet Microfluidics for High-Resolution Virology

Wenyang Jing and Hee-Sun Han

Anal. Chem., 2022, 94, 8085
DOI: 10.1021/acs.analchem.2c00615

Numerical and experimental investigation of a flow focusing droplet-based microfluidic device

Mostafa Soroor, Mohammad Zabetian Targhi and Seyed Ali Tabatabaei

European Journal of Mechanics - B/Fluids, 2021, 89, 289
DOI: 10.1016/j.euromechflu.2021.06.013

Flexible Generation of Multi‐Aqueous Core Hydrogel Capsules Using Microfluidic Aqueous Two‐Phase System

Hui Wang, Haitao Liu, Fan He, Wenwen Chen, Xu Zhang, Mengqian Zhao, Li Wang and Jianhua Qin

Adv Materials Technologies, 2020, 5
DOI: 10.1002/admt.202000045

Microfluidics-based generation of cell encapsulated microbeads in the presence of electric fields and spatio-temporal viability studies

Md Danish Eqbal, Farha Naaz, Kajal Sharma and Venkat Gundabala

Colloids and Surfaces B: Biointerfaces, 2021, 208, 112065
DOI: 10.1016/j.colsurfb.2021.112065

Simulation-based insights into cell encapsulation dynamics in droplet microfluidics

Kamal Jannati, Mohammad-Hassan Rahimian, Mehrdad Raisee and Azadeh Jafari

Physics of Fluids, 2024, 36
DOI: 10.1063/5.0203089

Rapid Microfluidic Formation of Uniform Patient-Derived Breast Tumor Spheroids

Zhuhao Wu, Zhiyi Gong, Zheng Ao, Junhua Xu, Hongwei Cai, Maram Muhsen, Samuel Heaps, Maria Bondesson, Shishang Guo and Feng Guo

ACS Appl. Bio Mater., 2020, 3, 6273
DOI: 10.1021/acsabm.0c00768

A portable droplet generation system for ultra-wide dynamic range digital PCR based on a vibrating sharp-tip capillary

Ziyi He, Jing Wang, Bethany J. Fike, Xiaojun Li, Chong Li, Balapuwaduge Lihini Mendis and Peng Li

Biosensors and Bioelectronics, 2021, 191, 113458
DOI: 10.1016/j.bios.2021.113458

Microfluidic encapsulation of supramolecular optical chemosensors for high-throughput analysis and screening

Huilin Wen, Haixia Shen, Yangteng Ou, Huan Shao, Minhui Li, Jing Zhang, Zhiqian Zhang and Ziyi Yu

Sensors and Actuators B: Chemical, 2022, 355, 131302
DOI: 10.1016/j.snb.2021.131302

Cell Culture in Microfluidic Droplets

Sébastien Sart, Gustave Ronteix, Shreyansh Jain, Gabriel Amselem and Charles N. Baroud

Chem. Rev., 2022, 122, 7061
DOI: 10.1021/acs.chemrev.1c00666

Biomaterials in droplet-based microfluidics: From structural design to biomedical applications

Andrea Fergola, Cesare Gabriele Gaglio, Simone Luigi Marasso, Matteo Cocuzza, Candido Fabrizio Pirri, Lucia Napione and Francesca Frascella

Materials Today Advances, 2025, 28, 100667
DOI: 10.1016/j.mtadv.2025.100667