Issue 39, 2024

CRISPR-Cas-based biosensors for the detection of cancer biomarkers

Abstract

Along with discovering cancer biomarkers, non-invasive detection methods have played a critical role in early cancer diagnosis and prognostic improvement. Some traditional detection methods have been used for detecting cancer biomarkers, but they are time-consuming and involve materials and human costs. With great flexibility, sensitivity and specificity, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated system provides a wide range of application prospects in this field. Herein, we introduce the background of the CRISPR-Cas (CRISPR-associated) system and comprehensively summarize the diagnosis strategies of cancer mediated by the CRISPR-Cas system, including four kinds of biochemical-based markers: nucleic acid, enzyme, tumor-specific protein and exosome. Furthermore, we discuss the challenges in implementing the CRISPR-Cas system in clinical applications.

Graphical abstract: CRISPR-Cas-based biosensors for the detection of cancer biomarkers

Article information

Article type
Critical Review
Submitted
01 Сер 2024
Accepted
27 Сер 2024
First published
28 Сер 2024

Anal. Methods, 2024,16, 6634-6653

CRISPR-Cas-based biosensors for the detection of cancer biomarkers

Y. Feng, J. Yang, Z. He, X. Liu and C. Ma, Anal. Methods, 2024, 16, 6634 DOI: 10.1039/D4AY01446D

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