Issue 4, 2019

Phenylboronic acid derivative-modified (6,5) single-wall carbon nanotube probes for detecting glucose and hydrogen peroxide

Abstract

In this paper, we presented a new method for constructing near-infrared fluorescence probes and their applications in detecting glucose and hydrogen peroxide (H2O2). We used purified (6,5) single-wall carbon nanotubes (SWCNTs) separated by a polyethylene glycol/dextran aqueous two-phase system as the basis for near-infrared probes. Different phenylboronic acids were used for non-covalent modification of SWCNTs (6,5). Glucose was detected by the specific binding of the boronic acid group with cis-diol. Hydrogen peroxide was detected by horseradish peroxidase (HRP) combined with phenylboronic-acid-modified SWCNTs. The results revealed that the fluorescence intensity of purified SWCNTs was significantly enhanced without other chiral nanotube interactions compared to that of the raw SWCNT material. The fluorescence responses of 3-carboxy-5-nitrophenylboronic acid-modified purified CNTs could be used to effectively measure glucose in the concentration range from 0.01 mM to 0.50 mM with an interval linear index of R2 = 0.996 (LOD = 1.7 μM, S/N = 3). The detected H2O2 with the 3-aminobenzeneboronic acid-modified (6,5) SWCNT with HRP was in the concentration range from 5.0 μM to 40 μM with an interval linear index of R2 = 0.997 (LOD = 0.85 μM, S/N = 3). Moreover, this sensor exhibited a strong anti-interference effect without biological matrix fluorescence effects above 1000 nm wavelength. Thus, the proposed novel near-infrared fluorescence probes employed for reducing the interference of the biomatrix in this region are expected to be used in subsequent biosensor investigations.

Graphical abstract: Phenylboronic acid derivative-modified (6,5) single-wall carbon nanotube probes for detecting glucose and hydrogen peroxide

Supplementary files

Article information

Article type
Paper
Submitted
09 Лис 2018
Accepted
21 Гру 2018
First published
16 Січ 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 2258-2267

Phenylboronic acid derivative-modified (6,5) single-wall carbon nanotube probes for detecting glucose and hydrogen peroxide

Y. Qiao, R. Zhao, M. Zhang, H. Zhang, Y. Wang and P. Hu, RSC Adv., 2019, 9, 2258 DOI: 10.1039/C8RA09272A

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