Issue 5, 2019

The anti-diarrhea activity of red algae-originated sulphated polysaccharides on ETEC-K88 infected mice

Abstract

Polysaccharides from red algae Porphyra haitanensis and Gracilaria lemaneiformis possess various bioactive functions, however, their anti-diarrhea activity remains incompletely defined. In the current study, sulphated polysaccharides were extracted by high pressure treatment plus ethanol precipitation from these two algae, and named PHSP(hp) and GLSP(hp), respectively. PHSP(hp) and GLSP(hp) showed decreased viscosity and molecular weight. Meanwhile, they have a certain immunomodulatory effect on wound healing and migration of RAW264.7 cells. Moreover, they significantly increased the secretion of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). A BALB/c model infected by enterotoxigenic Escherichia coli (ETEC)-K88 was also established to evaluate the anti-diarrhea activity of PHSP(hp) and GLSP(hp). The results showed that PHSP(hp) and GLSP(hp) were able to alleviate mice diarrhea symptoms. Meanwhile, they inhibited the release of pro-inflammatory cytokines and suppressed the secretion of immunoglobulin A via reducing the population of B cells. In addition, the nitroblue tetrazolium levels of mouse serum were decreased. Taken together, PHSP(hp) and GLSP(hp) alleviated the inflammatory response of ETEC-K88-induced diarrhea through both specific and non-specific immunity. Sulphated polysaccharides from red algae may be used as functional food components for remitting diarrhea.

Graphical abstract: The anti-diarrhea activity of red algae-originated sulphated polysaccharides on ETEC-K88 infected mice

Article information

Article type
Paper
Submitted
08 Лис 2018
Accepted
08 Січ 2019
First published
18 Січ 2019
This article is Open Access
Creative Commons BY license

RSC Adv., 2019,9, 2360-2370

The anti-diarrhea activity of red algae-originated sulphated polysaccharides on ETEC-K88 infected mice

B. Liu, Q. Liu, G. Li, L. Sun, Y. Gao, Y. Zhang, H. Liu, M. Cao and G. Liu, RSC Adv., 2019, 9, 2360 DOI: 10.1039/C8RA09247H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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