Issue 5, 2015

Thermoelectric lab-on-a-chip ELISA

Abstract

We report a new, thermoelectric method for performing enzyme-linked immunosorbent assay (ELISA) in a microfluidic device. The concentration of the analyte is determined by measuring the heat of an enzymatic reaction between glucose and glucose oxidase using thin-film antimony/bismuth thermopile. The feasibility of lab-on-a-chip thermoelectric ELISA is demonstrated by measuring the concentration of 8-hydroxy-2-deoxyguanosine (8OHdG) in urine samples from amyloid precursor protein transgenic mice. The detection method is based on formation of a complex between 8OHdG, anti-8OHdG capture antibody and glucose oxidase linked IgG antibody. The complex is immobilized at the lower channel wall of the microfluidic device, over the measuring junctions of the thermopile. The amount of heat detected by the thermoelectric sensor is inversely proportional to the concentration of 8OHdG. Standard calibration curve was created using synthetic 8OHdG. The regression line equation of the standard calibration curve was used to estimate the concentration of 8OHdG in mouse urine.

Graphical abstract: Thermoelectric lab-on-a-chip ELISA

Article information

Article type
Paper
Submitted
19 Лис 2014
Accepted
12 Січ 2015
First published
29 Січ 2015

Anal. Methods, 2015,7, 2055-2063

Author version available

Thermoelectric lab-on-a-chip ELISA

G. G. Nestorova, V. L. Kopparthy, N. D. Crews and E. J. Guilbeau, Anal. Methods, 2015, 7, 2055 DOI: 10.1039/C4AY02764G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements