Fe3O4@GO magnetic nanocomposites protect mesenchymal stem cells and promote osteogenic differentiation of rat bone marrow mesenchymal stem cells

Abstract

Fe₃O₄ nanoparticles (Fe₃O₄ NPs) are typical magnetic materials for bone tissue regeneration. However, the accompanying oxidative stress during the reaction process of Fe₃O₄ NPs and H₂O₂ in bone remodeling and disease may hinder their application. In order to reduce this side effect, we selected graphene oxide (GO) to modify Fe₃O₄ NPs. We showed that Fe₃O₄@GO magnetic nanocomposites (Fe₃O₄@GO MNCs) eliminated 30% of H₂O₂ in 3 h, and reduced the amount of ˙OH, the intermediate product of the Fenton reaction. The cellular study demonstrated that Fe₃O₄@GO MNCs reduced the cell damage caused by reactive oxygen species (ROS) and improved the activity of mesenchymal stem cells (MSCs). Moreover, when the magnetic field and bone morphogenetic protein-2 (BMP2) delivered by Fe₃O₄@GO MNCs worked together, osteogenic differentiation of MSCs in vitro was well promoted.