Issue 9, 2014

Enzymatic biosensors based on ingá-cipó peroxidase immobilised on sepiolite for TBHQ quantification

Abstract

Sepiolite clay mineral was used as a support for the immobilisation of the peroxidase enzyme from ingá-cipó (Inga edulis Mart.) and was used with graphite powder, multi-walled carbon nanotubes (CNTs), mineral oil, and nafion 0.5% (v/v) in the development of a new biosensor for the determination of the antioxidant tert-butylhydroquinone (TBHQ) by square-wave voltammetry (SWV). For the optimisation and application of the biosensor, several parameters were investigated to determine the optimum experimental conditions using SWV. The best performance was obtained using a 0.1 mol L−1 phosphate buffer solution (pH 7.0), 4.0 × 10−4 mol L−1 hydrogen peroxide, a frequency of 50 Hz, a pulse amplitude of 60 mV, and a scan increment of 6 mV. The biosensor showed good repeatability and reproducibility and remained stable for a period of 20 weeks. The analytical curve revealed a linear response range of 1.65 to 9.82 mg L−1 (r = 0.994) with detection and quantification limits of 0.41 and 1.25 mg L−1. A recovery study of TBHQ in salad dressing samples yielded values from 99.6–104.8%. The proposed biosensor was successfully used for the determination of TBHQ in commercial salad dressing samples, giving a relative error of 5.4% in relation to the comparative method (chromatographic).

Graphical abstract: Enzymatic biosensors based on ingá-cipó peroxidase immobilised on sepiolite for TBHQ quantification

Article information

Article type
Paper
Submitted
06 Січ 2014
Accepted
09 Лют 2014
First published
10 Лют 2014

Analyst, 2014,139, 2214-2220

Enzymatic biosensors based on ingá-cipó peroxidase immobilised on sepiolite for TBHQ quantification

T. Regina de Oliveira, G. F. Grawe, S. K. Moccelini, A. J. Terezo and M. Castilho, Analyst, 2014, 139, 2214 DOI: 10.1039/C4AN00028E

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