Issue 24, 2022

Rapid detection of phycotoxin domoic acid in seawater and seafood based on the developed lateral flow immunoassay

Abstract

A lateral flow immunoassay (LFIA) of phycotoxin domoic acid (DA) contaminating seawater and marine organisms was developed in this investigation. Nine clones of monoclonal antibodies against DA were produced and characterized. The test system was implemented in the indirect competitive format, where gold nanoparticles as a marker were conjugated with secondary antibodies. The developed test system allows for the detection of DA with a cutoff of 60 ng mL−1 and an instrumental detection limit of 1.4 ng mL−1 within 15 min. The LFIA was applied to detect DA in seawater, mussels, shrimps, and octopuses. A simple method of seafood sample preparation was proposed. The entire analytical cycle, from obtaining a sample to the estimation of final results, takes only 30 min. The assay recoveries ranged from 88.5% to 124%. The developed analytical method is a promising solution for rapid on-site monitoring of marine toxicants in water and food throughout the farm-to-fork chain.

Graphical abstract: Rapid detection of phycotoxin domoic acid in seawater and seafood based on the developed lateral flow immunoassay

Supplementary files

Article information

Article type
Paper
Submitted
06 Mud 2022
Accepted
08 Kho 2022
First published
14 Kho 2022

Anal. Methods, 2022,14, 2446-2452

Rapid detection of phycotoxin domoic acid in seawater and seafood based on the developed lateral flow immunoassay

O. D. Hendrickson, E. A. Zvereva, O. N. Solopova, N. E. Varlamov, O. B. Shemchukova, A. V. Zherdev, P. G. Sveshnikov and B. B. Dzantiev, Anal. Methods, 2022, 14, 2446 DOI: 10.1039/D2AY00751G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements