Issue 46, 2020

Coenzyme-dependent nanozymes playing dual roles in oxidase and reductase mimics with enhanced electron transport

Abstract

Although nanozymes overcome a series of shortcomings of natural enzymes, their wide applications are hampered due to their limited varieties. In this work, we propose a coenzyme-dependent nanozyme, a synergistic composite comprising zeolitic imidazolate frameworks encapsulated with polyethylenimine (PEI) and functionalized with a flavin mononucleotide (PEI/ZIF-FMN). The flavin mononucleotide (FMN) plays the role of a prosthetic group, and the positively charged NH2 groups in PEI readily provide the binding affinity to nicotinamide adenine dinucleotide (NADH), which facilitates the electron transfer from NADH to FMN and terminal electron acceptors (such as O2) with a greatly enhanced (80 times) catalytic performance. The integrated nanoparticle–coenzyme composite works as an NADH oxidase mimic and couples with dehydrogenases for the tandem enzymatic reaction. PEI/ZIF-FMN also mediated the electron transfer from NADH to cytochrome c (Cyt c), thereby exhibiting Cyt c reductase-like activity.

Graphical abstract: Coenzyme-dependent nanozymes playing dual roles in oxidase and reductase mimics with enhanced electron transport

Supplementary files

Article information

Article type
Paper
Submitted
13 Ndz 2020
Accepted
27 Nhl 2020
First published
28 Nhl 2020

Nanoscale, 2020,12, 23578-23585

Coenzyme-dependent nanozymes playing dual roles in oxidase and reductase mimics with enhanced electron transport

J. Chen, Q. Ma, M. Li, W. Wu, L. Huang, L. Liu, Y. Fang and S. Dong, Nanoscale, 2020, 12, 23578 DOI: 10.1039/D0NR06605B

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