A NIR fluorescent probe for the detection of renal damage based on overrepresentation of alanine aminopeptidase enzyme

Marcia Domínguez; Kathleen Meyer; Félix Sancenón; Juan F. Blandez; Manuel Serrano; Ramón Martínez-Máñez

doi:10.1039/D2CC05408F

A NIR fluorescent probe for the detection of renal damage based on overrepresentation of alanine aminopeptidase enzyme†

Marcia Domínguez,^ab Kathleen Meyer,^c Félix Sancenón,^abde Juan F. Blandez,*^abd Manuel Serrano*^cf and Ramón Martínez-Máñez

*^abde

Author affiliations

* Corresponding authors

^a Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Spain

^b CIBER de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain

^c Institute for Research in Biomedicine (IRB Barcelona), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain
E-mail: manuel.serrano@irbbarcelona.org

^d Unidad Mixta de Investigación en Nanomedicina y Sensores, Universitat Politècnica de València, Instituto de Investigación Sanitaria La Fe, Spain
E-mail: juablaba@upvnet.upv.es, rmaez@qim.upv.es

^e Unidad Mixta UPV-CIPF de Investigación en Mecanismos de Enfermedades y Nanomedicina, Universitat Politècnica de València, Centro de Investigación Príncipe Felipe, Spain

^f Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, Spain

Abstract

Kidney damage generates changes at the phenotypic and genotypic levels that allow its monitoring using different biomarkers in blood, urine or serum. Among these biomarkers, kidney failure causes the urine overrepresentation of the alanine aminopeptidase (APN) enzyme. Here, we describe the design of a molecular probe (NB-ALA) based on the Nile Blue fluorophore (NB), which can detect the APN enzyme in urine by simple fluorometric measurements.

This article is part of the themed collection: Chemosensors and Molecular Logic

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DOI: https://doi.org/10.1039/D2CC05408F
Article type: Communication
Submitted: 03 Nhl 2022
Accepted: 31 Sun 2023
First published: 31 Sun 2023
This article is Open Access

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Chem. Commun., 2023,59, 2481-2484

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A NIR fluorescent probe for the detection of renal damage based on overrepresentation of alanine aminopeptidase enzyme

M. Domínguez, K. Meyer, F. Sancenón, J. F. Blandez, M. Serrano and R. Martínez-Máñez, Chem. Commun., 2023, 59, 2481 DOI: 10.1039/D2CC05408F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

A NIR fluorescent probe for the detection of renal damage based on overrepresentation of alanine aminopeptidase enzyme†

Abstract

Supplementary files

Article information

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A NIR fluorescent probe for the detection of renal damage based on overrepresentation of alanine aminopeptidase enzyme

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