Issue 42, 2022

Development of monoclonal antibodies for the detection of diuron in water and sugarcane and their application in immunochromatographic strips

Abstract

Diuron (DR) as a chemical herbicide persists in soil and water for a long time and causes extensive harm to humans. We have produced an excellent monoclonal antibody (mAb) with the ability to sensitively and specifically recognize DR in water and sugarcane samples. The semi-inhibitory concentration (IC50) of this mAb was 0.28 ng mL−1, and the limit of detection (LOD) was 0.07 ng mL−1. Based on the mAb, an immunochromatographic assay (ICA) strip was developed. The visual detection limits of the strip assay were estimated, and the critical values of DR in water and sugarcane samples were determined to be 5 and 10 ng mL−1, respectively, when assessed by the naked eye. The ICA strip was validated by HPLC-MS for water and sugarcane samples which had been spiked with DR. This ICA strip could be a useful tool for in-site and rapid detection of DR in water and sugarcane samples.

Graphical abstract: Development of monoclonal antibodies for the detection of diuron in water and sugarcane and their application in immunochromatographic strips

Supplementary files

Article information

Article type
Paper
Submitted
22 Kho 2022
Accepted
19 Mha 2022
First published
06 Ndz 2022

Anal. Methods, 2022,14, 4202-4208

Development of monoclonal antibodies for the detection of diuron in water and sugarcane and their application in immunochromatographic strips

J. Zhou, J. Liu, S. Song, L. Liu, C. Xu, H. Kuang and W. Ma, Anal. Methods, 2022, 14, 4202 DOI: 10.1039/D2AY01006B

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