Issue 4, 2021

Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Abstract

We performed label-free imaging of human-hair medulla using multi-modal nonlinear optical microscopy. Intra-medulla lipids (IMLs) were clearly visualized by ultra-multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging. Two groups of IMLs were found: second harmonic generation (SHG) active and inactive. By combining SHG analysis with CARS, the two groups were identified as free fatty acids and wax esters, respectively.

Graphical abstract: Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

Supplementary files

Article information

Article type
Communication
Submitted
19 Ndz 2020
Accepted
22 N’w 2020
First published
23 N’w 2020

Analyst, 2021,146, 1163-1168

Visualizing intra-medulla lipids in human hair using ultra-multiplex CARS, SHG, and THG microscopy

D. Kaneta, M. Goto, M. Hagihara, P. Leproux, V. Couderc, M. Egawa and H. Kano, Analyst, 2021, 146, 1163 DOI: 10.1039/D0AN01880E

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