Issue 2, 2020

Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities

Abstract

Transient associations between numerous organelles—e.g., the endoplasmic reticulum and the mitochondria—forge highly-coordinated, particular environments essential for cross-compartment information flow. Our perspective summarizes chemical–biology tools that have enabled identifying proteins present within these itinerant communities against the bulk proteome, even when a particular protein's presence is fleeting/substoichiometric. However, proteins resident at these ephemeral junctions also experience transitory changes to their interactomes, small-molecule signalomes, and, importantly, functions. Thus, a thorough census of sub-organellar communities necessitates functionally probing context-dependent signaling properties of individual protein-players. Our perspective accordingly further discusses how repurposing of existing tools could allow us to glean a functional understanding of protein-specific signaling activities altered as a result of organelles pulling together. Collectively, our perspective strives to usher new chemical–biology techniques that could, in turn, open doors to modulate functions of specific subproteomes/organellar junctions underlying the nuanced regulatory subsystem broadly termed as contactology.

Graphical abstract: Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities

Article information

Article type
Review Article
Submitted
02 Dzi 2020
Accepted
16 Mud 2020
First published
27 Mud 2020
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2020,1, 42-55

Neighborhood watch: tools for defining locale-dependent subproteomes and their contextual signaling activities

M. J. C. Long, Y. Zhao and Y. Aye, RSC Chem. Biol., 2020, 1, 42 DOI: 10.1039/D0CB00041H

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