Issue 2, 2019

Engineering a stable future for DNA-origami as a biomaterial

Abstract

DNA as a biomaterial has evoked great interest as a potential platform for therapeutics and diagnostics and as hydrogel scaffolds due to the relative ease of programming its robust and uniform shape, site-specific functionality and controlled responsive behavior. However, for a stable self-assembled product, a relatively high cation concentration is required to prevent denaturation. Physiological and cell-culture conditions do not match these concentrations and present additional nucleases that cause a serious threat to the integrity of DNA-based materials. For the translation of this promising technology towards bioengineering challenges, stability needs to be guaranteed. Over the past years, various methods have been developed addressing the stability-related weaknesses of DNA-origami. This mini-review explains the common stability issues and compares the stabilization strategies recently developed. We present a detailed overview of each method in order to ease the selection process on which method to use for future users of DNA-origami as a biomaterial.

Graphical abstract: Engineering a stable future for DNA-origami as a biomaterial

Article information

Article type
Minireview
Submitted
06 Nhl 2018
Accepted
28 Huk 2018
First published
29 Huk 2018

Biomater. Sci., 2019,7, 532-541

Engineering a stable future for DNA-origami as a biomaterial

H. Bila, E. E. Kurisinkal and M. M. C. Bastings, Biomater. Sci., 2019, 7, 532 DOI: 10.1039/C8BM01249K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements