Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa

Rambabu Dandela; Danielle Mantin; Benjamin F. Cravatt; Josep Rayo; Michael M. Meijler

doi:10.1039/C7SC04287F

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Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa†

Rambabu Dandela,‡^a Danielle Mantin,

^a Benjamin F. Cravatt,^b Josep Rayo

*^a and Michael M. Meijler

*^a

Author affiliations

* Corresponding authors

^a Dept. of Chemistry, The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva, Israel
E-mail: peprayo@gmail.com, meijler@bgu.ac.il

^b The Skaggs Institute for Chemical Biology, Department of Molecular Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA

Abstract

The opportunistic human pathogen Pseudomonas aeruginosa secretes 2-heptyl-3-hydroxy-4-quinolone (PQS), a quorum sensing (QS) signal that regulates the expression of numerous virulence genes. Here we report the development and application of chemical probes to globally map quinolone binding proteins. The revealed quinolone interactome contains both known as well as newly identified virulence factors and presents new targets for the treatment of bacterial infections.

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DOI: https://doi.org/10.1039/C7SC04287F
Article type: Edge Article
Submitted: 02 Nhl 2017
Accepted: 19 Sun 2018
First published: 19 Sun 2018
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry

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Chem. Sci., 2018,9, 2290-2294

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Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa

R. Dandela, D. Mantin, B. F. Cravatt, J. Rayo and Michael M. Meijler, Chem. Sci., 2018, 9, 2290 DOI: 10.1039/C7SC04287F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Science

Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa†

Abstract

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Proteome-wide mapping of PQS-interacting proteins in Pseudomonas aeruginosa

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