Issue 95, 2017

Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing

Abstract

Methods to map small-molecule binding sites on cellular RNAs are important for understanding interactions with both endogenous and exogenous compounds. Pt(II) reagents are well-known DNA and RNA crosslinking agents, but sequence-specific and genome-wide identification of Pt targets following in-cell treatment is challenging. Here we describe application of high-throughput ‘Pt-Seq’ to identify Pt-rRNA adducts following treatment of S. cerevisiae with cisplatin.

Graphical abstract: Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing

Supplementary files

Article information

Article type
Communication
Submitted
12 Ndz 2017
Accepted
24 Nhl 2017
First published
27 Nhl 2017

Chem. Commun., 2017,53, 12746-12749

Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing

K. Plakos and V. J. DeRose, Chem. Commun., 2017, 53, 12746 DOI: 10.1039/C7CC06708A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements