Issue 60, 2016
From the journal:

Chemical Communications

Immobilization of an artificial imine reductase within silica nanoparticles improves its performance†

Martina Hestericová,a   M. Rita Correro,b   Markus Lenz,c   Philippe F.-X. Corvini,c   Patrick Shahgaldian*b  and  Thomas R. Ward*a  

* Corresponding authors

a Department of Chemistry, University of Basel, Spitalstrasse 51, CH-4056 Basel, Switzerland
E-mail: thomas.ward@unibas.ch

b Institute of Chemistry and Bioanalytics, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, CH-4132 Muttenz, Switzerland

c Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132 Muttenz, Switzerland

Abstract

Silica nanoparticles equipped with an artificial imine reductase display remarkable activity towards cyclic imine- and NAD+ reduction. The method, based on immobilization and protection of streptavidin on silica nanoparticles, shields the biotinylated metal cofactor against deactivation yielding over 46 000 turnovers in pure samples and 4000 turnovers in crude cellular extracts.

Graphical abstract: Immobilization of an artificial imine reductase within silica nanoparticles improves its performance

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Article information

DOI
https://doi.org/10.1039/C6CC04604E
Article type
Communication
Submitted
01 Kho 2016
Accepted
28 Kho 2016
First published
06 Maw 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 9462-9465

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Immobilization of an artificial imine reductase within silica nanoparticles improves its performance

M. Hestericová, M. R. Correro, M. Lenz, P. F.-X. Corvini, P. Shahgaldian and T. R. Ward, Chem. Commun., 2016, 52, 9462 DOI: 10.1039/C6CC04604E

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